Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,363,234 T→C T134A (ACC→GCC)  yfaY ← IPR008135 CinA family protein YfaY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,363,2340TC99.7% 1488.1 / ‑4.3 360T134A (ACC→GCC) yfaYIPR008135 CinA family protein YfaY
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (176/183);  total (176/184)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 372 total aligned reads displayed.

CAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGT  >  NC_000913/2363136‑2363331
                                                                                                  |                                                                                                 
 aGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGcc                                                                                                >  1:4527193/1‑101 (MQ=255)
    aTTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTAc                                                                                             <  1:4526879/101‑1 (MQ=255)
    aTTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTAc                                                                                             <  1:4526659/101‑1 (MQ=255)
    aTTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTAc                                                                                             >  1:4527199/1‑101 (MQ=255)
      ttCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCg                                                                                           >  1:4527202/1‑101 (MQ=255)
       tCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCgg                                                                                          <  2:4526490/101‑1 (MQ=255)
       tCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCgg                                                                                          <  2:4526534/101‑1 (MQ=255)
       tCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCgg                                                                                          <  2:4526972/101‑1 (MQ=255)
       tCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCATACCgg                                                                                          <  1:4526784/101‑1 (MQ=255)
        cGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCggg                                                                                         >  2:4527205/1‑101 (MQ=255)
         gTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGt                                                                                        <  1:4526435/101‑1 (MQ=255)
         gTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGt                                                                                        >  1:4527207/1‑101 (MQ=255)
            cTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTAt                                                                                     <  1:4526763/101‑1 (MQ=255)
            cTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTAt                                                                                     <  2:4526670/101‑1 (MQ=255)
            cTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTAt                                                                                     >  1:4527212/1‑101 (MQ=255)
             tCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTAtt                                                                                    >  2:4527216/1‑101 (MQ=255)
               gACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGa                                                                                  <  1:4527083/101‑1 (MQ=255)
                aCCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGAt                                                                                 >  1:4527221/1‑101 (MQ=255)
                aCCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGAt                                                                                 >  2:4527222/1‑101 (MQ=255)
                  cATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCTCCTACCGGGTTATTGATaa                                                                               >  1:4527225/1‑101 (MQ=255)
                   aTCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATaaa                                                                              <  1:4526728/101‑1 (MQ=255)
                     cACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAAtt                                                                            >  1:4527230/1‑101 (MQ=255)
                     cACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAAtt                                                                            <  2:4526864/101‑1 (MQ=255)
                         ttAAATTCTGACGGTACGGCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGcc                                                                                                <  2:4526793/77‑1 (MQ=255)
                         ttAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGc                                                                        >  1:4527236/1‑101 (MQ=255)
                           aaaTTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCAc                                                                      >  2:4527237/1‑101 (MQ=255)
                            aaTTCTGACGGTACGCCGGGAGTAAAGGACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACt                                                                     <  2:4526702/101‑1 (MQ=255)
                              ttCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTgg                                                                   <  2:4526686/101‑1 (MQ=255)
                                cTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTggca                                                                 >  2:4527244/1‑101 (MQ=255)
                                cTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTggca                                                                 >  1:4527242/1‑101 (MQ=255)
                                cTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTggca                                                                 <  2:4526732/101‑1 (MQ=255)
                                 tGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTggcag                                                                <  2:4526761/101‑1 (MQ=255)
                                 tGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCCGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTggcag                                                                <  1:4526855/101‑1 (MQ=255)
                                  gACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTggcagg                                                               >  1:4527249/1‑101 (MQ=255)
                                   aCGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTggcaggc                                                              >  2:4527252/1‑101 (MQ=255)
                                   aCGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTggcaggc                                                              <  2:4526723/101‑1 (MQ=255)
                                    cGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTggcaggca                                                             <  1:4526839/101‑1 (MQ=255)
                                      gTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGc                                                           >  2:4527258/1‑101 (MQ=255)
                                      gTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGc                                                           >  1:4527259/1‑101 (MQ=255)
                                        aCGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTc                                                         >  1:4527264/1‑101 (MQ=255)
                                         cGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTcc                                                        <  2:4526656/101‑1 (MQ=255)
                                         cGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTcc                                                        <  2:4526744/101‑1 (MQ=255)
                                            cGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTgg                                                                   <  1:4527070/87‑1 (MQ=255)
                                            cGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCt                                                     >  2:4527269/1‑101 (MQ=255)
                                             gggAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTa                                                                                      <  1:4526526/67‑1 (MQ=255)
                                             gggAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCtt                                                    >  1:4527270/1‑101 (MQ=255)
                                                aGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGtt                                                 >  1:4527276/1‑101 (MQ=255)
                                                aGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGtt                                                 >  2:4527280/1‑101 (MQ=255)
                                                 gTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGttt                                                >  1:4527283/1‑101 (MQ=255)
                                                  tAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTa                                               >  1:4527286/1‑101 (MQ=255)
                                                  tAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTa                                               <  1:4526825/101‑1 (MQ=255)
                                                   aaaGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTAc                                              <  2:4526608/101‑1 (MQ=255)
                                                   aaaGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTAc                                              <  1:4526617/101‑1 (MQ=255)
                                                   aaaGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTAc                                              >  2:4527292/1‑101 (MQ=255)
                                                   aaaGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTAc                                              <  1:4526908/101‑1 (MQ=255)
                                                   aaaGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTAc                                              >  1:4527293/1‑101 (MQ=255)
                                                     aGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACgg                                            >  2:4527300/1‑101 (MQ=255)
                                                      gAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGt                                           <  2:4526803/101‑1 (MQ=255)
                                                      gAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGt                                           >  1:4527302/1‑101 (MQ=255)
                                                       aaCATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGtt                                          <  1:4526861/101‑1 (MQ=255)
                                                        aCATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTg                                         <  2:4526930/101‑1 (MQ=255)
                                                         cATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGc                                        >  1:4527307/1‑101 (MQ=255)
                                                          aTCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCt                                       >  2:4527310/1‑101 (MQ=255)
                                                          aTCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCt                                       <  1:4526628/101‑1 (MQ=255)
                                                             aGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGtt                                          >  2:4527316/1‑95 (MQ=255)
                                                             aGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATCGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCgg                                    >  1:4527314/1‑101 (MQ=255)
                                                               gCAACGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTg                                  >  1:4527320/1‑101 (MQ=255)
                                                                 aaCGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGcc                                <  1:4526206/101‑1 (MQ=255)
                                                                  aCGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCa                               <  1:4526973/101‑1 (MQ=255)
                                                                   cGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGttt                                                >  1:4527326/1‑83 (MQ=255)
                                                                   cGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCAt                              <  2:4526952/101‑1 (MQ=255)
                                                                   cGATTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCAt                              <  1:4526775/101‑1 (MQ=255)
                                                                     aTTAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTa                            <  1:4526795/101‑1 (MQ=255)
                                                                      ttAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTAc                           >  2:4527330/1‑101 (MQ=255)
                                                                       tAAGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGTAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACt                          >  1:4527332/1‑101 (MQ=255)
                                                                        aaGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTc                         >  2:4527336/1‑101 (MQ=255)
                                                                        aaGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTc                         <  1:4526809/101‑1 (MQ=255)
                                                                         aGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCg                        <  2:4526769/101‑1 (MQ=255)
                                                                         aGCTGCACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCg                        >  1:4527338/1‑101 (MQ=255)
                                                                              cACGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCAc                   <  1:4527008/101‑1 (MQ=255)
                                                                               aCGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACg                  >  2:4527342/1‑101 (MQ=255)
                                                                                cGGCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGt                 <  1:4526743/101‑1 (MQ=255)
                                                                                 ggCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAAt                                                                             >  2:4527348/1‑40 (MQ=255)
                                                                                  gCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTc               >  1:4527349/1‑101 (MQ=255)
                                                                                  gCAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTc               <  1:4526937/101‑1 (MQ=255)
                                                                                   cAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCg              <  2:4526831/101‑1 (MQ=255)
                                                                                   cAAAACCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCg              <  2:4526713/101‑1 (MQ=255)
                                                                                    aaaaCCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGt                                           >  1:4527357/1‑71 (MQ=255)
                                                                                    aaaaCCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGt             >  2:4527354/1‑101 (MQ=255)
                                                                                    aaaaCCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGt             <  1:4526689/101‑1 (MQ=255)
                                                                                       aCCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGa          <  2:4526553/101‑1 (MQ=255)
                                                                                       aCCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGa          >  1:4527363/1‑101 (MQ=255)
                                                                                       aCCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGa          <  2:4526646/101‑1 (MQ=255)
                                                                                       aCCACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTAAgg                                            >  2:4527364/1‑67 (MQ=255)
                                                                                        ccACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGaa         <  1:4526875/101‑1 (MQ=255)
                                                                                        ccACAGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGaa         <  2:4526619/101‑1 (MQ=255)
                                                                                         cacaGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGaaa        <  1:4526704/101‑1 (MQ=255)
                                                                                           caGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATa      <  2:4526576/101‑1 (MQ=255)
                                                                                           caGGCGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATa      <  2:4527040/101‑1 (MQ=255)
                                                                                               cGGCGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGt  >  1:4527371/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGT  >  NC_000913/2363136‑2363331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑