BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,612,921	A→G	N341S (AAT→AGT)	hyfR →	DNA‑binding transcriptional activator HyfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,612,921	0	A	G	100.0%	1431.3 / NA	340	N341S (AAT→AGT)	hyfR	DNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (177/163); total (177/163)

Only 100 of 340 total aligned reads displayed.

TATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATT  >  NC_000913/2612823‑2613018

tATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTc                                                                                                 >  2:5007847/1‑101 (MQ=255)

 aTTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCt                                                                                                >  2:5007852/1‑101 (MQ=255)

  ttGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTg                                                                                               <  1:5007021/101‑1 (MQ=255)

   tGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTgg                                                                                              >  1:5007854/1‑101 (MQ=255)

     ccGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGc                                                                                            <  2:5007638/101‑1 (MQ=255)

      cGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTgg                                                                                              >  1:5007860/1‑98 (MQ=255)

      cGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCa                                                                                           <  2:5007670/101‑1 (MQ=255)

      cGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCa                                                                                           >  2:5007858/1‑101 (MQ=255)

       gTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCAt                                                                                          <  1:5007540/101‑1 (MQ=255)

        ttGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATc                                                                                         >  2:5007864/1‑101 (MQ=255)

           gCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGt                                                                                      >  2:5007869/1‑101 (MQ=255)

           gCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGt                                                                                      >  1:5007867/1‑101 (MQ=255)

            cAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTg                                                                                     <  2:5007366/101‑1 (MQ=255)

             aaTGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGa                                                                                    <  1:5007654/101‑1 (MQ=255)

              aTGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGAc                                                                                   >  1:5007878/1‑101 (MQ=255)

              aTGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGAc                                                                                   >  2:5007877/1‑101 (MQ=255)

                gCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACAt                                                                                 <  2:5007319/101‑1 (MQ=255)

                  cGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATta                                                                               >  2:5007886/1‑101 (MQ=255)

                  cGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATta                                                                               >  1:5007882/1‑101 (MQ=255)

                   gATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATtat                                                                              >  2:5007891/1‑101 (MQ=255)

                     tGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATtatct                                                                            <  1:5007280/101‑1 (MQ=255)

                      gCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATtatcta                                                                           >  2:5007895/1‑101 (MQ=255)

                       ccTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGt                                                                                      >  2:5007898/1‑89 (MQ=255)

                        cTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATtatctatc                                                                         <  2:5007518/101‑1 (MQ=255)

                        cTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATtatctatc                                                                         >  1:5007901/1‑101 (MQ=255)

                         tGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCa                                                                        <  1:5007112/101‑1 (MQ=255)

                           gCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCaaa                                                                      <  1:5007474/101‑1 (MQ=255)

                           gCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCaaa                                                                      >  1:5007906/1‑101 (MQ=255)

                            cGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAg                                                                     <  1:5007339/101‑1 (MQ=255)

                             gTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGc                                                                    <  2:5007248/101‑1 (MQ=255)

                              tAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGcc                                                                   >  2:5007911/1‑101 (MQ=255)

                               aGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCa                                                                  >  1:5007913/1‑101 (MQ=255)

                               aGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCa                                                                  <  1:5007700/101‑1 (MQ=255)

                                gCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAg                                                                 <  2:5007499/101‑1 (MQ=255)

                                 cATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAgg                                                                <  1:5007388/101‑1 (MQ=255)

                                  aTGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCTCCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCaaa                                                                      <  1:5007318/94‑1 (MQ=255)

                                  aTGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGc                                                               <  1:5007563/101‑1 (MQ=255)

                                   tGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCa                                                              <  2:5007299/101‑1 (MQ=255)

                                   tGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCa                                                              >  1:5007917/1‑101 (MQ=255)

                                    gACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCaa                                                             <  2:5007544/101‑1 (MQ=255)

                                      ccGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATg                                                           <  1:5007432/101‑1 (MQ=255)

                                      ccGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATg                                                           >  2:5007921/1‑101 (MQ=255)

                                       cGATTTGCAGGAAAGTTTGCAGCAAGAAAACCCCCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATgg                                                          <  2:5006999/101‑1 (MQ=255)

                                        gATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGa                                                         >  1:5007925/1‑101 (MQ=255)

                                        gATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGa                                                         <  2:5007374/101‑1 (MQ=255)

                                         aTTTGCAGGAAAGTTTGCAGCAAGAAAGCCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGaa                                                        <  1:5007520/101‑1 (MQ=255)

                                         aTTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGaa                                                        >  1:5007928/1‑101 (MQ=255)

                                          tttGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAAt                                                            <  2:5007460/96‑1 (MQ=255)

                                           ttGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACa                                                                                  <  2:5007146/73‑1 (MQ=255)

                                           ttGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGa                                                      >  1:5007934/1‑101 (MQ=255)

                                           ttGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGa                                                      >  2:5007933/1‑101 (MQ=255)

                                             gCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGAcc                                                    >  2:5007940/1‑101 (MQ=255)

                                              cAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCt                                                   >  1:5007941/1‑101 (MQ=255)

                                               aGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTg                                                  >  1:5007946/1‑101 (MQ=255)

                                               aGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTg                                                  <  2:5007589/101‑1 (MQ=255)

                                                ggAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGc                                                 <  2:5007287/101‑1 (MQ=255)

                                                 gAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCt                                                <  2:5007383/101‑1 (MQ=255)

                                                  aaaGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTc                                               >  1:5007949/1‑101 (MQ=255)

                                                   aaGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTcc                                              <  2:5007227/101‑1 (MQ=255)

                                                   aaGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTcc                                              <  1:5007481/101‑1 (MQ=255)

                                                   aaGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTcc                                              <  1:5007410/101‑1 (MQ=255)

                                                   aaGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTcc                                              >  2:5007954/1‑101 (MQ=255)

                                                    aGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCca                                             <  2:5007264/101‑1 (MQ=255)

                                                      tttGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagc                                           <  2:5007395/101‑1 (MQ=255)

                                                      tttGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagc                                           >  1:5007956/1‑101 (MQ=255)

                                                      tttGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagc                                           <  1:5007357/101‑1 (MQ=255)

                                                        tGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagcag                                         <  2:5007427/101‑1 (MQ=255)

                                                        tGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagcag                                         >  2:5007959/1‑101 (MQ=255)

                                                           agcaAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTa                                      >  2:5007963/1‑101 (MQ=255)

                                                              aaGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGat                                   >  1:5007968/1‑101 (MQ=255)

                                                                 aaaaCCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATAtt                                >  2:5007972/1‑101 (MQ=255)

                                                                  aaaCCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATtg                               >  1:5007973/1‑101 (MQ=255)

                                                                   aaCCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATtgt                              >  2:5007977/1‑101 (MQ=255)

                                                                   aaCCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATtgt                              >  1:5007979/1‑101 (MQ=255)

                                                                   aaCCACCAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATtgt                              <  2:5007331/101‑1 (MQ=255)

                                                                    accaccAGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATtgtg                             <  2:5007735/101‑1 (MQ=255)

                                                                     ccaccaGCTTACCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTgg                            >  1:5007984/1‑101 (MQ=255)

                                                                       accaGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCg                          <  2:5007399/101‑1 (MQ=255)

                                                                        ccaGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCg                          <  2:5007601/100‑1 (MQ=255)

                                                                         caGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGaa                        >  2:5007986/1‑101 (MQ=255)

                                                                          aGCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAg                                                                     >  2:5007990/1‑55 (MQ=255)

                                                                           gCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAaga                      <  1:5007242/101‑1 (MQ=255)

                                                                           gCTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAaga                      <  1:5007445/101‑1 (MQ=255)

                                                                            cTTAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAagag                     <  1:5007591/101‑1 (MQ=255)

                                                                             ttAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGc                    >  1:5007996/1‑101 (MQ=255)

                                                                             ttAGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGc                    >  2:5007994/1‑101 (MQ=255)

                                                                               aGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATAtt                                <  1:5007512/87‑1 (MQ=255)

                                                                               aGCGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGa                  >  1:5008001/1‑101 (MQ=255)

                                                                                 cGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAaga                      <  2:5007578/95‑1 (MQ=255)

                                                                                 cGAGCAGCTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACa                >  2:5008004/1‑101 (MQ=255)

                                                                                   agcagcTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGc                           >  2:5008008/1‑88 (MQ=255)

                                                                                      agcTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACg           >  2:5008011/1‑101 (MQ=255)

                                                                                       gcTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACgg          >  1:5008015/1‑101 (MQ=255)

                                                                                        cTCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGgt         <  2:5007488/101‑1 (MQ=255)

                                                                                         tCCTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGgtg        >  2:5008019/1‑101 (MQ=255)

                                                                                           cTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGtt      <  2:5007437/101‑1 (MQ=255)

                                                                                           cTTTCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGtt      >  1:5008021/1‑101 (MQ=255)

                                                                                            tttCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTg     <  1:5007890/101‑1 (MQ=255)

                                                                                            tttCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTg     >  2:5008024/1‑101 (MQ=255)

                                                                                             ttCGAGTCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGa    <  2:5007087/101‑1 (MQ=255)

TATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATT  >  NC_000913/2612823‑2613018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`