Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,839,028 T→C L79L (TTA→TTG)  ascG ← DNA‑binding transcriptional repressor AscG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,839,0280TC99.7% 1519.0 / NA 363L79L (TTA→TTG) ascGDNA‑binding transcriptional repressor AscG
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (187/175);  total (188/175)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 363 total aligned reads displayed.

TACTGAATCGCCTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGTAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGCCAGCAAGTTTG  >  NC_000913/2838930‑2839125
                                                                                                  |                                                                                                 
tACTGAATCGCCTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCaa                                                                                                 <  2:5440374/101‑1 (MQ=255)
tACTGAATCGCCTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCaa                                                                                                 <  2:5440621/101‑1 (MQ=255)
  cTGAATCGCCTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAAtt                                                                                               >  1:5441086/1‑101 (MQ=255)
     aaTCGCCTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCAc                                                                                            >  2:5441092/1‑101 (MQ=255)
     aaTCGCCTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCAc                                                                                            >  1:5441091/1‑101 (MQ=255)
       tCGCCTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTa                                                                                          <  1:5440704/101‑1 (MQ=255)
        cGCCTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTaa                                                                                         <  1:5440939/101‑1 (MQ=255)
         gCCTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTaaa                                                                                        >  2:5441098/1‑101 (MQ=255)
          ccTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTaaaa                                                                                       <  1:5440785/101‑1 (MQ=255)
           cTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAaaat                                                                                      <  1:5440528/101‑1 (MQ=255)
            tGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAaaata                                                                                     >  2:5441102/1‑101 (MQ=255)
             ggCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAaaataa                                                                                    <  1:5440693/101‑1 (MQ=255)
             ggCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAaaataa                                                                                    <  1:5440828/101‑1 (MQ=255)
              gcgcTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAaaataaa                                                                                   >  1:5441103/1‑101 (MQ=255)
               cgcTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAaaataaat                                                                                  <  2:5440585/101‑1 (MQ=255)
                 ctctTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGc                                                                                >  2:5441110/1‑101 (MQ=255)
                 ctctTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGc                                                                                >  1:5441111/1‑101 (MQ=255)
                  tcttcttctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGcc                                                                               <  2:5440737/101‑1 (MQ=255)
                  tcttcttctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGcc                                                                               <  1:5440422/101‑1 (MQ=255)
                   cttcttctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCg                                                                              >  2:5441114/1‑101 (MQ=255)
                    ttcttctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGt                                                                             >  2:5441120/1‑101 (MQ=255)
                     tcttctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTg                                                                            <  2:5440644/101‑1 (MQ=255)
                     tcttctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTg                                                                            <  1:5440273/101‑1 (MQ=255)
                      cttctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTgg                                                                           <  1:5440548/101‑1 (MQ=255)
                      cttctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTgg                                                                           <  1:5440498/101‑1 (MQ=255)
                      cttctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTgg                                                                           >  1:5441123/1‑101 (MQ=255)
                        tctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTa                                                                         <  2:5440514/101‑1 (MQ=255)
                        tctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTa                                                                         >  2:5441127/1‑101 (MQ=255)
                         ctGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTaa                                                                        >  1:5441129/1‑101 (MQ=255)
                           gcgcTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAg                                                                      <  2:5440125/101‑1 (MQ=255)
                           gcgcTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAg                                                                      <  2:5440677/101‑1 (MQ=255)
                            cgcTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGc                                                                     >  2:5441133/1‑101 (MQ=255)
                               tgtgTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCgtg                                                                  >  1:5441142/1‑101 (MQ=255)
                               tgtgTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCgtg                                                                  >  2:5441143/1‑101 (MQ=255)
                               tgtgTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCgtg                                                                  >  1:5441138/1‑101 (MQ=255)
                                gtgtTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCgtgt                                                                 <  2:5440431/101‑1 (MQ=255)
                                  gtTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTg                                                               >  1:5441149/1‑101 (MQ=255)
                                   tttACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTgg                                                              >  2:5441151/1‑101 (MQ=255)
                                      aCCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCa                                                           <  2:5440810/101‑1 (MQ=255)
                                       ccATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCAc                                                          >  2:5441155/1‑101 (MQ=255)
                                        cATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACt                                                         <  1:5440852/101‑1 (MQ=255)
                                         aTCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTa                                                        >  1:5441159/1‑101 (MQ=255)
                                            tGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCa                                                     <  2:5440755/101‑1 (MQ=255)
                                            tGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCa                                                     >  1:5441164/1‑101 (MQ=255)
                                               cAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGcc                                                  <  2:5440481/101‑1 (MQ=255)
                                                aaTAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGccc                                                 >  2:5441169/1‑101 (MQ=255)
                                                aaTAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGccc                                                 <  2:5440630/101‑1 (MQ=255)
                                                aaTAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGccc                                                 >  1:5441172/1‑101 (MQ=255)
                                                   aGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAg                                              <  2:5440542/101‑1 (MQ=255)
                                                   aGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAg                                              >  2:5441175/1‑101 (MQ=255)
                                                    gCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGc                                             <  1:5440561/101‑1 (MQ=255)
                                                    gCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGc                                             <  1:5440457/101‑1 (MQ=255)
                                                      aaCTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGt                                           >  1:5441177/1‑101 (MQ=255)
                                                       aCTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTc                                          >  2:5441182/1‑101 (MQ=255)
                                                        cTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGcccc                                                >  1:5441185/1‑94 (MQ=255)
                                                        cTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCt                                         <  1:5440660/101‑1 (MQ=255)
                                                        cTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCt                                         <  2:5440789/101‑1 (MQ=255)
                                                         tGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTg                                        >  2:5441190/1‑101 (MQ=255)
                                                         tGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTg                                        >  1:5441191/1‑101 (MQ=255)
                                                           ccgccCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAg                                      >  1:5441195/1‑101 (MQ=255)
                                                            cgccCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGt                                     <  2:5440535/101‑1 (MQ=255)
                                                            cgccCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGt                                     <  2:5440567/101‑1 (MQ=255)
                                                             gccCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTa                                    <  1:5440767/101‑1 (MQ=255)
                                                             gccCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTa                                    <  2:5440502/101‑1 (MQ=255)
                                                             gccCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTa                                    >  1:5441200/1‑101 (MQ=255)
                                                              cccTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCgtgt                                                                 >  2:5441202/1‑71 (MQ=255)
                                                                cTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTActc                                 >  1:5441205/1‑101 (MQ=255)
                                                                  ttCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCtt                               <  1:5440603/101‑1 (MQ=255)
                                                                  ttCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCtt                               >  1:5441213/1‑101 (MQ=255)
                                                                  ttCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCtt                               <  2:5440832/101‑1 (MQ=255)
                                                                  ttCTCTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCtt                               >  2:5441211/1‑101 (MQ=255)
                                                                   tctcTTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTc                              <  1:5440610/101‑1 (MQ=255)
                                                                    ctctTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTc                              >  2:5441217/1‑100 (MQ=255)
                                                                     tctTCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGc                            <  1:5440368/101‑1 (MQ=255)
                                                                       ttCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCg                          <  1:5440902/101‑1 (MQ=255)
                                                                       ttCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGNTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCg                          <  1:5440753/101‑1 (MQ=255)
                                                                        tCCGCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGa                         >  2:5441221/1‑101 (MQ=255)
                                                                           gCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAg                      >  2:5441227/1‑101 (MQ=255)
                                                                           gCCATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAg                      >  1:5441228/1‑101 (MQ=255)
                                                                            ccATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGa                     <  2:5440723/101‑1 (MQ=255)
                                                                            ccATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGa                     <  1:5440930/101‑1 (MQ=255)
                                                                             cATTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGAt                    >  1:5441231/1‑101 (MQ=255)
                                                                              aTTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGAtt                   <  2:5440927/101‑1 (MQ=255)
                                                                              aTTCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGAtt                   >  2:5441234/1‑101 (MQ=255)
                                                                                tCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTgc                 >  1:5441239/1‑101 (MQ=255)
                                                                                tCGCGCGGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTgc                 <  2:5440668/101‑1 (MQ=255)
                                                                                  gcgcgGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTgcgc               <  1:5440593/101‑1 (MQ=255)
                                                                                   cgcgGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTgcgcg              >  2:5441243/1‑101 (MQ=255)
                                                                                    gcgGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTgcgcgc             <  1:5440632/101‑1 (MQ=255)
                                                                                    gcgGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTgcgcgc             <  2:5441014/101‑1 (MQ=255)
                                                                                    gcgGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTgcgcgc             >  2:5441248/1‑101 (MQ=255)
                                                                                     cgGCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGcc            >  1:5441250/1‑101 (MQ=255)
                                                                                      ggCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGCCa           <  2:5440682/101‑1 (MQ=255)
                                                                                       gCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGCCAg          <  1:5440726/101‑1 (MQ=255)
                                                                                       gCATGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGCCAg          >  2:5441253/1‑101 (MQ=255)
                                                                                         aTGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGCCAGCa        >  1:5441259/1‑101 (MQ=255)
                                                                                          tGAAAGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGCCAGCaa       >  2:5441261/1‑101 (MQ=255)
                                                                                            aaaGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGCCAGCAAGt     >  1:5441267/1‑101 (MQ=255)
                                                                                            aaaGAGCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGCCAGCAAGt     <  2:5440862/101‑1 (MQ=255)
                                                                                              agagCAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGCCAGCAAGttt   >  1:5441270/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TACTGAATCGCCTGGCGCTCTTCTTCTGCGCTGTGTTTACCATCTGCCAATAGCAACTGCCGCCCTTTCTCTTCCGCCATTCGCGCGGCATGAAAGAGTAATTCACTAAAATAAATGCCGTGGTAAAGCGTGTTGGTCACTACCAGCCCCAGCGTCTGAGTACTCTTCGCCGACAGATTGCGCGCCAGCAAGTTTG  >  NC_000913/2838930‑2839125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑