Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,345,926 T→C S404P (TCC→CCC)  rpoN → RNA polymerase, sigma 54 (sigma N) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,345,9260TC99.7% 1323.1 / NA 317S404P (TCC→CCC) rpoNRNA polymerase, sigma 54 (sigma N) factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (180/136);  minor base A (1/0);  total (181/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

CCAGGCTGTCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAGCGAAACC  >  NC_000913/3345829‑3346023
                                                                                                 |                                                                                                 
ccAGGCTGTCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCa                                                                                                <  2:6446666/101‑1 (MQ=255)
 cAGGCTGTCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAg                                                                                               <  2:6446694/101‑1 (MQ=255)
  aGGCTGTCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGt                                                                                              >  1:6447145/1‑101 (MQ=255)
    gCTGTCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCa                                                                                            <  1:6446975/101‑1 (MQ=255)
     cTGTCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCAc                                                                                           >  2:6447149/1‑101 (MQ=255)
         cGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCa                                                                                       <  2:6447015/101‑1 (MQ=255)
          gAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCa                                                                                            <  1:6446662/95‑1 (MQ=255)
          gAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCaa                                                                                      >  1:6447154/1‑101 (MQ=255)
          gAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCaa                                                                                      <  1:6446612/101‑1 (MQ=255)
           aaaTGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAAt                                                                                     >  1:6447160/1‑101 (MQ=255)
           aaaTGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAAt                                                                                     >  2:6447155/1‑101 (MQ=255)
            aaTGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATa                                                                                    <  2:6446720/101‑1 (MQ=255)
             aTGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATAc                                                                                   >  2:6447162/1‑101 (MQ=255)
             aTGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATAc                                                                                   <  2:6446716/101‑1 (MQ=255)
             aTGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATAc                                                                                   <  2:6446479/101‑1 (MQ=255)
               gCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCg                                                                                 >  2:6447167/1‑101 (MQ=255)
                cATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGa                                                                                >  1:6447168/1‑101 (MQ=255)
                cATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGa                                                                                <  1:6446600/101‑1 (MQ=255)
                 aTGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAg                                                                               <  1:6446388/101‑1 (MQ=255)
                 aTGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAg                                                                               <  2:6446801/101‑1 (MQ=255)
                    aaTCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGggc                                                                            >  1:6447174/1‑101 (MQ=255)
                    aaTCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGggc                                                                            >  2:6447176/1‑101 (MQ=255)
                        gacgaTATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGggcggcg                                                                        <  1:6446677/101‑1 (MQ=255)
                        gacgaTATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGggcggcg                                                                        >  1:6447181/1‑101 (MQ=255)
                        gacgaTATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGggcggcg                                                                        >  2:6447180/1‑101 (MQ=255)
                          cgaTATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGggcggcggc                                                                      <  1:6446764/101‑1 (MQ=255)
                           gaTATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGgcggcggcg                                                                     >  2:6447186/1‑101 (MQ=255)
                            atatCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAggg                                                                             <  1:6446632/92‑1 (MQ=255)
                            atatCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGa                                                                    >  1:6447189/1‑101 (MQ=255)
                             tatCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGggc                                                                            >  1:6447194/1‑92 (MQ=255)
                             tatCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGaa                                                                   >  2:6447193/1‑101 (MQ=255)
                                ctcGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCt                                                                >  2:6447200/1‑101 (MQ=255)
                                 tcGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCtt                                                               <  2:6446741/101‑1 (MQ=255)
                                  cgcgTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGACACGTCaa                                                                                      >  2:6447203/1‑77 (MQ=255)
                                   gcgTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTtcc                                                             >  1:6447204/1‑101 (MQ=255)
                                     gTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTtcctc                                                           >  1:6447208/1‑101 (MQ=255)
                                       gACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCa                                                         >  1:6447213/1‑101 (MQ=255)
                                        aCCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCAc                                                        <  1:6446815/101‑1 (MQ=255)
                                           aCGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGc                                                     <  1:6446908/101‑1 (MQ=255)
                                           aCGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGc                                                     >  2:6447217/1‑101 (MQ=255)
                                           aCGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGc                                                     <  2:6446910/101‑1 (MQ=255)
                                           aCGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGc                                                     >  1:6447216/1‑101 (MQ=255)
                                               aaaaaTACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGAtt                                                 >  2:6447223/1‑101 (MQ=255)
                                                aaaaTACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTc                                                >  1:6447225/1‑101 (MQ=255)
                                                aaaaTACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTc                                                >  2:6447228/1‑101 (MQ=255)
                                                  aaTACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGt                                              >  1:6447231/1‑101 (MQ=255)
                                                    tACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTgc                                            >  1:6447236/1‑101 (MQ=255)
                                                     aCCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATAc                                                                                   <  2:6446525/61‑1 (MQ=255)
                                                     aCCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTgcg                                           >  2:6447239/1‑101 (MQ=255)
                                                       cTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCt                                         >  2:6447243/1‑101 (MQ=255)
                                                       cTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCt                                         >  1:6447244/1‑101 (MQ=255)
                                                          cATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGt                                      <  2:6446845/101‑1 (MQ=255)
                                                          cATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGt                                      <  1:6447047/101‑1 (MQ=255)
                                                          cATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGt                                      >  2:6447248/1‑101 (MQ=255)
                                                           aTAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTgcgc                                          >  2:6447254/1‑96 (MQ=255)
                                                           aTAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTg                                     >  1:6447251/1‑101 (MQ=255)
                                                            tAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTga                                    <  1:6446750/101‑1 (MQ=255)
                                                             aGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTgaa                                   >  2:6447258/1‑101 (MQ=255)
                                                              gTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTgaag                                  >  1:6447261/1‑101 (MQ=255)
                                                                ccACGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTgaagaa                                >  2:6447263/1‑101 (MQ=255)
                                                                   cGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAAtt                             <  1:6446713/101‑1 (MQ=255)
                                                                   cGAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAAtt                             >  2:6447266/1‑101 (MQ=255)
                                                                    gAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTa                            >  1:6447270/1‑101 (MQ=255)
                                                                    gAGGCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTa                            <  2:6446948/101‑1 (MQ=255)
                                                                      ggCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAAt                          <  1:6446943/101‑1 (MQ=255)
                                                                      ggCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAAt                          <  1:6446857/101‑1 (MQ=255)
                                                                      ggCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAAt                          >  1:6447275/1‑101 (MQ=255)
                                                                       gCATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATc                         <  1:6446546/101‑1 (MQ=255)
                                                                        cATTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATcg                        >  1:6447278/1‑101 (MQ=255)
                                                                         aTTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATcgc                       >  2:6447281/1‑101 (MQ=255)
                                                                         aTTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATcgc                       <  1:6446864/101‑1 (MQ=255)
                                                                         aTTTTTGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATcgc                       <  2:6446595/101‑1 (MQ=255)
                                                                          tttttGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATcgcg                      >  1:6447284/1‑101 (MQ=255)
                                                                           ttttGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCgcgg                     >  2:6447289/1‑101 (MQ=255)
                                                                            tttGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCgcggc                    <  2:6447138/101‑1 (MQ=255)
                                                                              tGAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGcggcgg                  >  1:6447292/1‑101 (MQ=255)
                                                                               gAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTa                            <  1:6446890/90‑1 (MQ=255)
                                                                               gAACTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGa                 >  1:6447295/1‑101 (MQ=255)
                                                                                aaCTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGaa                >  2:6447296/1‑101 (MQ=255)
                                                                                aaCTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGaa                <  1:6447141/101‑1 (MQ=255)
                                                                                 aCTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGaaa               <  2:6446833/101‑1 (MQ=255)
                                                                                 aCTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGaaa               <  2:6446630/101‑1 (MQ=255)
                                                                                  cTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGaaaa              >  1:6447301/1‑101 (MQ=255)
                                                                                  cTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGaaaa              <  2:6446871/101‑1 (MQ=255)
                                                                                  cTGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGaaaa              <  2:6446752/101‑1 (MQ=255)
                                                                                   tGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAAc             >  2:6447302/1‑101 (MQ=255)
                                                                                   tGAAGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAAc             <  2:6446656/101‑1 (MQ=255)
                                                                                     aaGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAAccc           <  1:6446826/101‑1 (MQ=255)
                                                                                      aGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCgcggcg                   >  1:6447309/1‑92 (MQ=255)
                                                                                      aGTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCa          >  2:6447308/1‑101 (MQ=255)
                                                                                       gTATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAg         <  2:6446775/101‑1 (MQ=255)
                                                                                        tATTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAGc        <  2:6446760/101‑1 (MQ=255)
                                                                                         aTTTCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAGCg       <  1:6446646/101‑1 (MQ=255)
                                                                                          tttctttcCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTtcctc                                                           >  2:6447320/1‑48 (MQ=255)
                                                                                          tttctttcCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAGCGa      >  1:6447315/1‑101 (MQ=255)
                                                                                          tttctttcCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAGCGa      >  2:6447317/1‑101 (MQ=255)
                                                                                            tCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAGCGaaa    <  2:6446819/101‑1 (MQ=255)
                                                                                            tCTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAGCGaaa    >  1:6447323/1‑101 (MQ=255)
                                                                                             cTTTCCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAGCGAAAc   >  1:6447327/1‑101 (MQ=255)
                                                                                              tttcCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAGCGAAAcc  <  2:6446682/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
CCAGGCTGTCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAGTATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATTAATCGCGGCGGAAAACCCAGCGAAACC  >  NC_000913/3345829‑3346023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑