Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,113,979 (C)5→4 coding (374/810 nt) pppA ← prepilin peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,113,9750C.100.0% 1514.3 / NA 289coding (378/810 nt)pppAprepilin peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (154/135);  total (154/135)

Only 100 of 311 total aligned reads displayed.

TCCACAATACGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGA  >  NC_000913/3113875‑3114071
                                                                                                    |                                                                                                
tCCACAATACGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGc                                                                                                  <  2:5248759/101‑1 (MQ=255)
 ccACAATACGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGcc                                                                                                 >  2:5249478/1‑101 (MQ=255)
  cacaATACGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGccc                                                                                                <  1:5248930/101‑1 (MQ=255)
   acaATACGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGcccc                                                                                               >  1:5249480/1‑101 (MQ=255)
     aaTACGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGccccat                                                                                             >  1:5249483/1‑99 (MQ=255)
     aaTACGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGccccat                                                                                             <  1:5248981/101‑3 (MQ=255)
      aTACGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGccccatc                                                                                            >  2:5249487/1‑98 (MQ=255)
       tACGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGccccatcc                                                                                           >  2:5249491/1‑97 (MQ=255)
        aCGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCa                                                                                         >  1:5249492/1‑101 (MQ=255)
          gCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACt                                                                                       >  1:5249497/1‑101 (MQ=255)
             cTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTc                                                                                    >  2:5249501/1‑101 (MQ=255)
              tGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCt                                                                                   >  2:5249504/1‑101 (MQ=255)
              tGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCt                                                                                   >  1:5249507/1‑101 (MQ=255)
               gAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCt                                                                                   <  2:5248862/100‑1 (MQ=255)
               gAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTg                                                                                  >  2:5249512/1‑101 (MQ=255)
               gAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTg                                                                                  <  1:5249015/101‑1 (MQ=255)
               gAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTg                                                                                  >  1:5249510/1‑101 (MQ=255)
                  tAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGcc                                                                               <  2:5249112/101‑1 (MQ=255)
                   aaaaaCATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGcca                                                                              >  1:5249516/1‑101 (MQ=255)
                   aaaaaCATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGcca                                                                              >  1:5249519/1‑101 (MQ=255)
                    aaaaCATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGccag                                                                             >  1:5249523/1‑101 (MQ=255)
                     aaaCATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGccaga                                                                            <  1:5248748/101‑1 (MQ=255)
                     aaaCATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGccaga                                                                            >  2:5249524/1‑101 (MQ=255)
                         aTCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGCTAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGccaga                                                                            >  1:5249533/1‑97 (MQ=255)
                         aTCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGccagaccag                                                                        >  2:5249529/1‑101 (MQ=255)
                         aTCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGccagaccag                                                                        <  1:5249038/101‑1 (MQ=255)
                         aTCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGccagaccag                                                                        >  1:5249530/1‑101 (MQ=255)
                            gggCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACt                                                                     >  2:5249536/1‑101 (MQ=255)
                                 gCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCa                                                                >  1:5249541/1‑101 (MQ=255)
                                   cATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGc                                                              <  1:5248915/101‑1 (MQ=255)
                                    aTTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCa                                                             >  2:5249544/1‑101 (MQ=255)
                                    aTTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCa                                                             <  1:5248950/101‑1 (MQ=255)
                                    aTTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCa                                                             >  1:5249546/1‑101 (MQ=255)
                                     ttGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCaa                                                            <  2:5248984/101‑1 (MQ=255)
                                     ttGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCaa                                                            >  1:5249551/1‑101 (MQ=255)
                                      tGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCaaa                                                           <  1:5249084/101‑1 (MQ=255)
                                        gTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCaaaaa                                                         <  1:5248640/101‑1 (MQ=255)
                                        gTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCaaaaa                                                         >  1:5249554/1‑101 (MQ=255)
                                        gTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCaaaaa                                                         <  2:5249222/101‑1 (MQ=255)
                                         tGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCaaaaaa                                                        <  1:5248999/101‑1 (MQ=255)
                                           aTCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGc                                                      >  1:5249558/1‑101 (MQ=255)
                                           aTCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGc                                                      <  2:5248965/101‑1 (MQ=255)
                                            tCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCa                                                     >  2:5249561/1‑101 (MQ=255)
                                              gAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAg                                                   >  1:5249564/1‑101 (MQ=255)
                                               aGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGccc                                                                                                <  2:5248913/56‑1 (MQ=255)
                                               aGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGt                                                  <  2:5249161/101‑1 (MQ=255)
                                               aGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGt                                                  <  2:5249008/101‑1 (MQ=255)
                                                ggTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGc                                                                                                  >  2:5249568/1‑53 (MQ=255)
                                                ggTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCACAAAAGCAAGTg                                                 <  1:5249131/101‑1 (MQ=255)
                                                 gTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGc                                                <  2:5249037/101‑1 (MQ=255)
                                                  tCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCt                                               >  1:5249569/1‑101 (MQ=255)
                                                   cGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTg                                              >  1:5249574/1‑101 (MQ=255)
                                                   cGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTg                                              <  2:5249049/101‑1 (MQ=255)
                                                   cGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTg                                              <  1:5248809/101‑1 (MQ=255)
                                                    gATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGt                                             >  1:5249577/1‑101 (MQ=255)
                                                    gATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGt                                             <  1:5249234/101‑1 (MQ=255)
                                                     aTGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTc                                            >  1:5249581/1‑101 (MQ=255)
                                                      tGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCa                                           >  2:5249583/1‑101 (MQ=255)
                                                        acacTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAg                                                               <  2:5248842/79‑1 (MQ=255)
                                                        acacTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAAt                                         <  1:5249221/101‑1 (MQ=255)
                                                         cacTCGCGGCAATCAGCCAGGCGGATAATATCATCGCCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATa                                        <  1:5249103/101‑1 (MQ=255)
                                                         cacTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATa                                        >  1:5249585/1‑101 (MQ=255)
                                                          acTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATaa                                       <  2:5249146/101‑1 (MQ=255)
                                                             cgcgGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTc                                    <  1:5249366/101‑1 (MQ=255)
                                                               cgGCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCAc                                                                                        <  2:5249064/47‑1 (MQ=39)
                                                                ggCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTccac                                 >  1:5249591/1‑101 (MQ=255)
                                                                 gCAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTccacc                                <  2:5249298/101‑1 (MQ=255)
                                                                  cAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTccacca                               >  2:5249592/1‑101 (MQ=255)
                                                                  cAATCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTccacca                               <  2:5248816/101‑1 (MQ=255)
                                                                   aaTCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAg                              >  1:5249597/1‑101 (MQ=255)
                                                                    aTCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGc                             >  1:5249600/1‑101 (MQ=255)
                                                                    aTCAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGc                             <  1:5249164/101‑1 (MQ=255)
                                                                      cAGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCAACCAGCgg                           >  2:5249602/1‑101 (MQ=255)
                                                                       aGCCAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGc                             >  1:5249606/1‑98 (MQ=255)
                                                                         ccAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATa                        <  1:5249054/101‑1 (MQ=255)
                                                                         ccAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATa                        >  2:5249609/1‑101 (MQ=255)
                                                                          cAGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATaa                       <  1:5249122/101‑1 (MQ=255)
                                                                           aGGCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAAc                      >  1:5249611/1‑101 (MQ=255)
                                                                            ggCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACg                     <  2:5249258/101‑1 (MQ=255)
                                                                            ggCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACg                     >  1:5249614/1‑101 (MQ=255)
                                                                             gCGGATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGc                    >  2:5249617/1‑101 (MQ=255)
                                                                               ggATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCtt                  >  2:5249621/1‑101 (MQ=255)
                                                                               ggATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACACCACCAGCGGATAACGCtt                  >  1:5249622/1‑101 (MQ=255)
                                                                                gATAATATCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTg                 <  1:5248962/101‑1 (MQ=255)
                                                                                 ataataTCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGc                >  2:5249626/1‑101 (MQ=255)
                                                                                   gatatCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTg              >  1:5249629/2‑101 (MQ=255)
                                                                                     tatCATCACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAATGCTTGCTGAt            >  2:5249633/1‑101 (MQ=255)
                                                                                      atcatcACCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAtt           >  1:5249634/1‑101 (MQ=255)
                                                                                        catcaCCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAtttt         >  2:5249638/1‑101 (MQ=255)
                                                                                        catcaCCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAtttt         <  1:5249143/101‑1 (MQ=255)
                                                                                        catcaCCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAtttt         <  2:5249024/101‑1 (MQ=255)
                                                                                         atcaCCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTc        <  1:5248855/101‑1 (MQ=255)
                                                                                          tctccGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGAt                         <  1:5249065/80‑1 (MQ=255)
                                                                                          tcaCCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGAt                         <  2:5249199/83‑1 (MQ=255)
                                                                                          tcaCCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCg       <  1:5249342/101‑1 (MQ=255)
                                                                                          tcaCCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCg       >  1:5249643/1‑101 (MQ=255)
                                                                                           caCCGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCaaaa                                                          <  2:5248790/49‑1 (MQ=39)
                                                                                             ccGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGc      <  2:5249078/99‑1 (MQ=255)
                                                                                             ccGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCt    >  1:5249646/1‑101 (MQ=255)
                                                                                             ccGCCAG‑CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCt    <  2:5249105/101‑1 (MQ=255)
                                                                                                    |                                                                                                
TCCACAATACGCCCTGAGTAAAAACATCGGGCAGCCATTGGTGATCGAGGTCGATGACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGA  >  NC_000913/3113875‑3114071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑