Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,786,454 T→C Y399H (TAT→CAT)  gpmM → 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,786,4540TC100.0% 1318.1 / NA 320Y399H (TAT→CAT) gpmM2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (151/169);  total (151/169)

Only 100 of 320 total aligned reads displayed.

CTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGC  >  NC_000913/3786356‑3786551
                                                                                                  |                                                                                                 
ctacctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCAt                                                                                                 <  1:6434562/101‑1 (MQ=255)
ctacctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCAt                                                                                                 >  1:6435223/1‑101 (MQ=255)
ctacctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCAt                                                                                                 <  1:6434807/101‑1 (MQ=255)
ctacctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCAt                                                                                                 <  1:6434774/101‑1 (MQ=255)
  acctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATcc                                                                                               >  2:6435227/1‑101 (MQ=255)
   cctacGATGTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCg                                                                                              >  1:6435228/1‑101 (MQ=255)
   cctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATcc                                                                                               <  2:6434398/100‑1 (MQ=255)
     tacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATcc                                                                                               <  1:6434516/98‑1 (MQ=255)
     tacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGaa                                                                                            >  2:6435235/1‑101 (MQ=255)
     tacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGaa                                                                                            >  1:6435234/1‑101 (MQ=255)
      acGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAAc                                                                                           <  1:6434756/101‑1 (MQ=255)
        gATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACgg                                                                                         <  2:6434709/101‑1 (MQ=255)
         aTCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGc                                                                                        >  1:6435240/1‑101 (MQ=255)
           cTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGa                                                                                      >  2:6435244/1‑101 (MQ=255)
           cTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGa                                                                                      <  2:6434957/101‑1 (MQ=255)
               aaCCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATg                                                                                  <  1:6434654/101‑1 (MQ=255)
                aCCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATgg                                                                                 <  1:6434684/101‑1 (MQ=255)
                 ccGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATgg                                                                                 >  2:6435249/1‑100 (MQ=255)
                 ccGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGt                                                                                <  1:6434764/101‑1 (MQ=255)
                 ccGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGt                                                                                <  1:6434931/101‑1 (MQ=255)
                  cGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTa                                                                               <  1:6434595/101‑1 (MQ=255)
                   ggAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAg                                                                              <  2:6435045/101‑1 (MQ=255)
                   ggAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAg                                                                              >  2:6435253/1‑101 (MQ=255)
                   ggAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAg                                                                              >  1:6435252/1‑101 (MQ=255)
                      aaTGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTc                                                                           <  2:6434978/101‑1 (MQ=255)
                      aaTGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTc                                                                           >  2:6435257/1‑101 (MQ=255)
                       aTGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTca                                                                          <  1:6434987/101‑1 (MQ=255)
                         gAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTcaca                                                                        >  1:6435265/1‑101 (MQ=255)
                         gAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTcaca                                                                        >  2:6435264/1‑101 (MQ=255)
                          aGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCacac                                                                       <  1:6434835/101‑1 (MQ=255)
                            cTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCg                                                                     >  2:6435269/1‑101 (MQ=255)
                               cGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCgggg                                                                  >  2:6435272/1‑101 (MQ=255)
                                gCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCTGGGt                                                                 <  2:6434819/101‑1 (MQ=255)
                                 cAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCg                                                                     >  1:6435277/1‑96 (MQ=255)
                                 cAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTg                                                                >  2:6435276/1‑101 (MQ=255)
                                 cAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTg                                                                <  1:6434498/101‑1 (MQ=255)
                                  agagCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGa                                                               >  2:6435280/1‑101 (MQ=255)
                                   gagCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCgggg                                                                  >  2:6435285/1‑97 (MQ=255)
                                    agCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATg                                                             <  2:6434739/101‑1 (MQ=255)
                                    agCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATg                                                             <  2:6434614/101‑1 (MQ=255)
                                      cTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATc                                                                                                >  2:6435290/1‑64 (MQ=255)
                                      cTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGa                                                           >  1:6435289/1‑101 (MQ=255)
                                       tGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGaa                                                          >  2:6435293/1‑101 (MQ=255)
                                        gACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAg                                                         <  1:6434718/101‑1 (MQ=255)
                                          ccGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAgcg                                                       >  2:6435297/1‑101 (MQ=255)
                                            gAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAgcggc                                                     >  1:6435302/1‑101 (MQ=255)
                                            gAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAgcggc                                                     <  1:6434882/101‑1 (MQ=255)
                                             aaaaaCTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCGCACCGGGGTGATGGAAgcggcg                                                    <  2:6434768/101‑1 (MQ=255)
                                                  cTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTaa                                               <  2:6434808/101‑1 (MQ=255)
                                                  cTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTaa                                               >  2:6435305/1‑101 (MQ=255)
                                                   tGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTaaa                                              <  1:6434781/101‑1 (MQ=255)
                                                       tGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTg                                                                >  2:6435310/1‑79 (MQ=255)
                                                        gCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGt                                         <  1:6434905/101‑1 (MQ=255)
                                                        gCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGt                                         >  1:6435311/1‑101 (MQ=255)
                                                         cGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGtt                                        <  2:6434998/101‑1 (MQ=255)
                                                          ggCCATCAAGAGCGGCAAATACGACATCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTg                                       <  1:6434874/101‑1 (MQ=255)
                                                            ccATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGaa                                     <  2:6434909/101‑1 (MQ=255)
                                                             cATCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGAt                                                              <  2:6434758/75‑1 (MQ=255)
                                                              aTCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGtt                                        <  2:6434869/96‑1 (MQ=255)
                                                              aTCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAg                                    >  2:6435316/1‑100 (MQ=255)
                                                               tCAAGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAgcg                                  <  2:6434721/101‑1 (MQ=255)
                                                                 aaGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCt                                >  1:6435319/1‑101 (MQ=255)
                                                                 aaGAGCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCt                                <  2:6434834/101‑1 (MQ=255)
                                                                  agagCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTg                               >  2:6435320/1‑101 (MQ=255)
                                                                    agCGGCAAATACGACACCATCATCTGTAACCATCCGTACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGa                             >  2:6435326/1‑101 (MQ=255)
                                                                    agCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGa                             <  2:6434854/101‑1 (MQ=255)
                                                                    agCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGa                             <  1:6435056/101‑1 (MQ=255)
                                                                    agCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGa                             >  1:6435325/1‑101 (MQ=255)
                                                                     gCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGAt                            <  2:6434745/101‑1 (MQ=255)
                                                                     gCGGCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGAt                            <  1:6434860/101‑1 (MQ=255)
                                                                        gCAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCAc                         <  1:6434850/101‑1 (MQ=255)
                                                                         cAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACt                        <  2:6434801/101‑1 (MQ=255)
                                                                         cAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACt                        >  2:6435331/1‑101 (MQ=255)
                                                                         cAAATACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACt                        >  1:6435332/1‑101 (MQ=255)
                                                                           aaTACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACt                        <  1:6434740/99‑1 (MQ=255)
                                                                           aaTACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGc                      >  1:6435338/1‑101 (MQ=255)
                                                                           aaTACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGc                      <  2:6434736/101‑1 (MQ=255)
                                                                            aTACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAg                                             >  2:6435342/1‑77 (MQ=255)
                                                                             tACGACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGt                    >  1:6435343/1‑101 (MQ=255)
                                                                                gACACCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTg                                                                >  2:6435348/1‑54 (MQ=255)
                                                                                 acacCATCATCTGTAACCATCCGAACGGCGACATGGt                                                                                >  1:6435349/1‑37 (MQ=255)
                                                                                 acacCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAg                                    <  1:6434702/81‑1 (MQ=255)
                                                                                 acacCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGgaa                <  1:6435088/101‑1 (MQ=255)
                                                                                 acacCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGgaa                <  1:6434788/101‑1 (MQ=255)
                                                                                  cacCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGgaag               <  2:6434924/101‑1 (MQ=255)
                                                                                   acCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGgaaga              >  1:6435356/1‑101 (MQ=255)
                                                                                   acCATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGgaaga              >  2:6435353/1‑101 (MQ=255)
                                                                                    ccATCATCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGgaagaa             <  2:6434696/101‑1 (MQ=255)
                                                                                     catcatCTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGaagaag            >  1:6435359/1‑101 (MQ=255)
                                                                                      atcatcTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGt           <  1:6434964/101‑1 (MQ=255)
                                                                                       tcatcTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTc          <  2:6434659/101‑1 (MQ=255)
                                                                                        catcTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTcg         >  2:6435361/1‑101 (MQ=255)
                                                                                         atcTGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTcgc        <  2:6434885/101‑1 (MQ=255)
                                                                                           cTGTAACCATCCGAACGGCGACATGGTAGGTCCCACCGGGGTGATGGAAGCGGCGGTTAAAg                                             <  2:6434561/62‑1 (MQ=255)
                                                                                            tGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGaa     <  2:6435215/101‑1 (MQ=255)
                                                                                            tGTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGaa     <  2:6434685/101‑1 (MQ=255)
                                                                                             gTAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAATAAGTCGCGaaa    <  1:6435261/101‑1 (MQ=255)
                                                                                              tAACCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAg   >  2:6435367/1‑101 (MQ=255)
                                                                                               aaCCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGGGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGc  >  1:6435368/1‑101 (MQ=255)
                                                                                               aaCCATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGc  >  1:6435372/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGC  >  NC_000913/3786356‑3786551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑