BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	126,180	G→A	V162V (GTG→GTA)	aceF →	pyruvate dehydrogenase, E2 subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	126,180	0	G	A	100.0%	1443.6 / NA	336	V162V (GTG→GTA)	aceF	pyruvate dehydrogenase, E2 subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (166/170); total (166/170)

Only 100 of 343 total aligned reads displayed.

AAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAG  >  NC_000913/126082‑126277

 aaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAAc                                                                                                >  1:219213/1‑101 (MQ=255)

 aaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAAc                                                                                                <  2:218640/101‑1 (MQ=255)

  aGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACg                                                                                               >  2:219215/1‑101 (MQ=255)

    ttGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTg                                                                                             <  2:218774/101‑1 (MQ=255)

     tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTgg                                                                                            <  2:218693/101‑1 (MQ=255)

     tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTgg                                                                                            >  2:219220/1‑101 (MQ=255)

      gAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTggg                                                                                           <  1:218735/101‑1 (MQ=255)

      gAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTggg                                                                                           <  1:218679/101‑1 (MQ=255)

       aaGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTggg                                                                                           >  2:219223/1‑100 (MQ=255)

        aGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAAc                                                                                                <  1:218753/94‑1 (MQ=255)

        aGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTg                                                                                         >  1:219225/1‑101 (MQ=255)

           tGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACa                                                                                      >  1:219231/1‑101 (MQ=255)

              aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAg                                                                                   >  2:219233/1‑101 (MQ=255)

              aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAg                                                                                   <  2:218393/101‑1 (MQ=255)

              aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAg                                                                                   <  2:218621/101‑1 (MQ=255)

                  tCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTc                                                                               <  1:218311/101‑1 (MQ=255)

                   cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCt                                                                              <  1:218647/101‑1 (MQ=255)

                    gCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGAc                                                                                       <  1:218562/91‑1 (MQ=255)

                    gCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTa                                                                             <  2:218503/101‑1 (MQ=255)

                    gCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTa                                                                             >  2:219238/1‑101 (MQ=255)

                     cTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGt                                                                                          >  1:219243/1‑87 (MQ=255)

                      tGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTAcc                                                                           <  2:218557/101‑1 (MQ=255)

                      tGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTAcc                                                                           <  1:218877/101‑1 (MQ=255)

                        aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCgg                                                                         >  1:219247/1‑101 (MQ=255)

                        aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCgg                                                                         >  2:219248/1‑101 (MQ=255)

                         tCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGc                                                                        <  2:218593/101‑1 (MQ=255)

                         tCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGc                                                                        <  1:218711/101‑1 (MQ=255)

                          cACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTg                                                                                             >  2:219255/1‑79 (MQ=255)

                          cACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCt                                                                       <  1:218585/101‑1 (MQ=255)

                           aCCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTgg                                                                                            >  1:219256/1‑79 (MQ=255)

                           aCCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTc                                                                      <  1:218979/101‑1 (MQ=255)

                            ccGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCg                                                                     <  2:218662/101‑1 (MQ=255)

                             cGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTc                                                                      >  2:219259/1‑99 (MQ=255)

                              gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCt                                                                   <  2:218754/101‑1 (MQ=255)

                              gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCt                                                                   <  2:218802/101‑1 (MQ=255)

                                aGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGa                                                                 >  1:219264/1‑101 (MQ=255)

                                  aaGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCgg                                                                         >  2:219269/1‑91 (MQ=255)

                                  aaGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGAtt                                                               <  1:219121/101‑1 (MQ=255)

                                    ggCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTAt                                                             <  1:218839/101‑1 (MQ=255)

                                      cGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGa                                                                                        >  2:219276/1‑72 (MQ=255)

                                      cGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTc                                                                      >  2:219273/1‑90 (MQ=255)

                                      cGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATgg                                                           <  1:218814/101‑1 (MQ=255)

                                       gACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGt                                                          >  1:219280/1‑101 (MQ=255)

                                       gACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGt                                                          >  1:219277/1‑101 (MQ=255)

                                        aCAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTc                                                         <  1:218718/101‑1 (MQ=255)

                                         cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAg                                                                                   <  1:218639/74‑1 (MQ=255)

                                          aaGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCtt                                                       >  2:219285/1‑101 (MQ=255)

                                           aGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTc                                                      <  2:218533/101‑1 (MQ=255)

                                            ggCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCg                                                     <  2:218888/101‑1 (MQ=255)

                                             gCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCt                                                                       >  1:219291/1‑82 (MQ=255)

                                             gCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTg                                                                  >  2:219289/1‑87 (MQ=255)

                                              cTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGaa                                                   <  2:218608/101‑1 (MQ=255)

                                              cTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGaa                                                   <  1:218459/101‑1 (MQ=255)

                                                tCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCg                                                     >  2:219296/1‑97 (MQ=255)

                                                tCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGt                                                 <  2:218787/101‑1 (MQ=255)

                                                 cTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTc                                                <  1:218766/101‑1 (MQ=255)

                                                 cTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTc                                                <  2:218943/101‑1 (MQ=255)

                                                  tATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcg                                               <  2:218998/101‑1 (MQ=255)

                                                   aTGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgc                                              >  1:219299/1‑101 (MQ=255)

                                                     ggAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTc                                                                      >  2:219303/1‑75 (MQ=255)

                                                      gAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCggg                                           <  1:218599/101‑1 (MQ=255)

                                                      gAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCggg                                           <  1:218508/101‑1 (MQ=255)

                                                       aaGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCt                                                                   >  1:219304/1‑76 (MQ=255)

                                                       aaGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGt                                          <  1:218385/101‑1 (MQ=255)

                                                        aGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTg                                         <  1:218776/101‑1 (MQ=255)

                                                         gTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGa                                        <  1:218923/101‑1 (MQ=255)

                                                          ttCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGAtt                                                               >  2:219311/1‑77 (MQ=255)

                                                           tCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAgt                                                                                  >  1:219313/1‑57 (MQ=255)

                                                            ccGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGc                                     <  2:219016/101‑1 (MQ=255)

                                                              ggCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAg                                   >  1:219316/1‑101 (MQ=255)

                                                                cTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGgc                                 <  2:218845/101‑1 (MQ=255)

                                                                 tCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTc                                                                      >  2:219322/1‑63 (MQ=255)

                                                                 tCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAgg                                  >  1:219321/1‑99 (MQ=255)

                                                                 tCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGgcg                                <  1:218797/101‑1 (MQ=255)

                                                                    gTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACaaa                                                                                    <  1:218573/46‑1 (MQ=255)

                                                                    gTTTGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCgg                             >  1:219326/1‑101 (MQ=255)

                                                                      ttGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTc                                                                      >  2:219331/1‑58 (MQ=255)

                                                                      ttGCTGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCggggg                                         >  1:219330/1‑85 (MQ=255)

                                                                          tGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTc                                                                      >  1:219341/1‑54 (MQ=255)

                                                                          tGGCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGGGAAGCAgg                                  >  2:219338/1‑90 (MQ=255)

                                                                           ggCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTcc                      <  2:218862/101‑1 (MQ=255)

                                                                            gCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCggg                                           >  2:219343/1‑79 (MQ=255)

                                                                            gCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCg                     <  2:219122/101‑1 (MQ=255)

                                                                            gCACCGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCg                     <  1:218667/101‑1 (MQ=255)

                                                                               ccGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCtt                                                       >  2:219347/1‑64 (MQ=255)

                                                                               ccGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGcc                  <  2:218951/101‑1 (MQ=255)

                                                                               ccGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGcc                  <  2:218744/101‑1 (MQ=255)

                                                                                cGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGTGTGAAGCAGGCGCGGCAGCTCCGGCCg                 <  1:218865/101‑1 (MQ=255)

                                                                                cGTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGgc                                 >  1:219350/1‑85 (MQ=255)

                                                                                 gTGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCggg                                           >  2:219352/1‑74 (MQ=255)

                                                                                  tGAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCt               <  1:218887/101‑1 (MQ=255)

                                                                                   gAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTcc                      >  1:219361/1‑93 (MQ=255)

                                                                                   gAAAGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTa              >  1:219358/1‑101 (MQ=255)

                                                                                     aaGAGATCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAgg                                  >  2:219363/1‑79 (MQ=255)

                                                                                        agaTCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCt                                                                   >  2:219369/1‑43 (MQ=255)

                                                                                          aTCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGc                >  1:219371/1‑92 (MQ=255)

                                                                                           tCAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGaa      >  1:219375/1‑101 (MQ=255)

                                                                                            cAAAGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAag     >  1:219378/1‑101 (MQ=255)

                                                                                             aaaGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAagc    <  1:218948/101‑1 (MQ=255)

                                                                                              aaGTAAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAagca   <  2:218700/101‑1 (MQ=255)

AAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAG  >  NC_000913/126082‑126277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`