BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,339,842	A→G	A171A (GCA→GCG)	pgpB →	phosphatidylglycerophosphatase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,339,842	0	A	G	100.0%	1088.4 / NA	265	A171A (GCA→GCG)	pgpB	phosphatidylglycerophosphatase B
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (153/112); total (153/112)

Only 100 of 266 total aligned reads displayed.

GAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCCTGCTGC  >  NC_000913/1339744‑1339939

gAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGct                                                                                                 >  1:2251157/1‑101 (MQ=255)

gAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGct                                                                                                 <  2:2250628/101‑1 (MQ=255)

 aaGAGAAAAATATCCTACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGctg                                                                                                <  1:2250818/101‑1 (MQ=255)

 aaGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGctg                                                                                                <  2:2250410/101‑1 (MQ=255)

  agagAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGctgc                                                                                               >  1:2251161/1‑101 (MQ=255)

    agaAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGctgctg                                                                                             <  1:2250964/101‑1 (MQ=255)

     gaAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTgg                                                                                            >  2:2251166/1‑101 (MQ=255)

       aaaaTATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGcc                                                                                          >  1:2251170/1‑101 (MQ=255)

       aaaaTATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGACGTTTGCTGCCAGTTGGGCGCTGCTGGcc                                                                                          >  2:2251169/1‑101 (MQ=255)

        aaaTATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCg                                                                                         <  2:2251032/101‑1 (MQ=255)

         aaTATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGt                                                                                        >  2:2251173/1‑101 (MQ=255)

          atatCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGtt                                                                                       >  1:2251175/1‑101 (MQ=255)

           tatCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTg                                                                                      >  2:2251179/1‑101 (MQ=255)

            atCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTgg                                                                                     >  1:2251180/1‑101 (MQ=255)

             tCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGt                                                                                    <  1:2250803/101‑1 (MQ=255)

             tCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGt                                                                                    >  1:2251183/1‑101 (MQ=255)

               ccACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGttt                                                                                  >  2:2251188/1‑101 (MQ=255)

               ccACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGtt                                                                                   >  2:2251190/1‑100 (MQ=255)

                cacaATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTg                                                                                 >  1:2251192/1‑101 (MQ=255)

                 acaATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGc                                                                                >  2:2251196/1‑101 (MQ=255)

                  caATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCt                                                                               >  1:2251198/1‑101 (MQ=255)

                    atatTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGGCACACGATGTTTGCTGCCAGTTGGTCGCTGCTGGCCGTTGGTTTGCtgt                                                                             >  1:2251202/1‑101 (MQ=255)

                     tatTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCtgtg                                                                            >  2:2251204/1‑101 (MQ=255)

                     tatTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCtgtg                                                                            <  1:2250562/101‑1 (MQ=255)

                      atTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTgg                                                                           >  1:2251207/1‑101 (MQ=255)

                       tttGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGc                                                                          <  2:2250841/101‑1 (MQ=255)

                        ttGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGcc                                                                         >  2:2251210/1‑101 (MQ=255)

                        ttGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGcc                                                                         <  2:2250707/101‑1 (MQ=255)

                         tGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCcg                                                                        <  2:2250698/101‑1 (MQ=255)

                         tGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCcg                                                                        >  1:2251211/1‑101 (MQ=255)

                            gTTCACACTGGCAGTAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGt                                                                     >  1:2251216/1‑101 (MQ=255)

                            gTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGt                                                                     >  2:2251219/1‑101 (MQ=255)

                            gTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGt                                                                     >  1:2251221/1‑101 (MQ=255)

                             ttCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTc                                                                    <  2:2251098/101‑1 (MQ=255)

                              tCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCg                                                                   >  2:2251224/1‑101 (MQ=255)

                              tCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCg                                                                   <  1:2250705/101‑1 (MQ=255)

                               cacaCTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCgg                                                                  >  2:2251226/1‑101 (MQ=255)

                               cacaCTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCgg                                                                  <  1:2250939/101‑1 (MQ=255)

                                acacTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGc                                                                 >  1:2251228/1‑101 (MQ=255)

                                  acTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGa                                                               >  2:2251232/1‑101 (MQ=255)

                                    tGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCtggg                                                                            >  2:2251237/1‑84 (MQ=255)

                                    tGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAAc                                                             >  1:2251236/1‑101 (MQ=255)

                                     ggCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACg                                                            >  1:2251239/1‑101 (MQ=255)

                                        aGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTa                                                         <  1:2250754/101‑1 (MQ=255)

                                         gAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTaa                                                        <  1:2250625/101‑1 (MQ=255)

                                          aaaGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAAc                                                       >  1:2251243/1‑101 (MQ=255)

                                          aaaGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAAc                                                       <  2:2250782/101‑1 (MQ=255)

                                            agagACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCa                                                     >  2:2251246/1‑101 (MQ=255)

                                             gagaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCAt                                                    <  1:2250927/101‑1 (MQ=255)

                                              agaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCAtt                                                   <  2:2250798/101‑1 (MQ=255)

                                               gaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTg                                                  >  1:2251249/1‑101 (MQ=255)

                                               gaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTg                                                  <  1:2250760/101‑1 (MQ=255)

                                               gaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTg                                                  >  2:2251250/1‑101 (MQ=255)

                                               gaCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTg                                                  <  1:2250481/101‑1 (MQ=255)

                                                  ggggTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTa                                               <  2:2250722/101‑1 (MQ=255)

                                                   gggTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTAt                                              >  1:2251254/1‑101 (MQ=255)

                                                     gTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCt                                            >  2:2251255/1‑101 (MQ=255)

                                                     gTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCt                                            <  2:2250776/101‑1 (MQ=255)

                                                     gTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCt                                            <  2:2250827/101‑1 (MQ=255)

                                                      tttGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCtt                                           <  1:2250695/101‑1 (MQ=255)

                                                       ttGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTg                                          >  1:2251258/1‑101 (MQ=255)

                                                         gCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCt                                        >  2:2251261/1‑101 (MQ=255)

                                                         gCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCt                                        <  1:2250864/101‑1 (MQ=255)

                                                          ccTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTg                                       <  1:2251130/101‑1 (MQ=255)

                                                            tttCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCcgcg                                                                      >  2:2251266/1‑68 (MQ=255)

                                                            tttCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGt                                     >  1:2251265/1‑101 (MQ=255)

                                                            tttCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGt                                     <  1:2251026/101‑1 (MQ=255)

                                                             ttccttccGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTc                                    >  1:2251268/1‑101 (MQ=255)

                                                             ttccttccGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTc                                    <  1:2250668/101‑1 (MQ=255)

                                                               ccttccGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTg                                  >  2:2251273/1‑101 (MQ=255)

                                                               ccttccGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTg                                  <  1:2250896/101‑1 (MQ=255)

                                                                cttccGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTgg                                 >  1:2251274/1‑101 (MQ=255)

                                                                  tccGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGc                               >  1:2251278/1‑101 (MQ=255)

                                                                  tccGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGc                               <  1:2250877/101‑1 (MQ=255)

                                                                   ccGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTgg                                 >  2:2251280/1‑98 (MQ=255)

                                                                   ccGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCa                              <  1:2250777/101‑1 (MQ=255)

                                                                   ccGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTAGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCa                              <  2:2250750/101‑1 (MQ=255)

                                                                    cGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCaa                             <  1:2250916/101‑1 (MQ=255)

                                                                    cGGTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCaa                             >  1:2251281/1‑101 (MQ=255)

                                                                     ggTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAAc                            <  1:2250737/101‑1 (MQ=255)

                                                                      gTCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACg                           <  2:2250832/101‑1 (MQ=255)

                                                                       tCACACGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACgg                          >  1:2251284/1‑101 (MQ=255)

                                                                        cacaCGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACggg                         >  1:2251287/1‑101 (MQ=255)

                                                                         acacGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGttt                                                                                  <  1:2250849/43‑1 (MQ=255)

                                                                          cacGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTCCTATCTTGCTGGTCTGGGCAACGGGAg                       >  2:2251290/1‑101 (MQ=255)

                                                                           acGATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGt                      >  1:2251292/1‑101 (MQ=255)

                                                                             gATGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCa                    <  1:2250906/101‑1 (MQ=255)

                                                                              aTGTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCAt                   >  1:2251296/1‑101 (MQ=255)

                                                                                gTTTGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACggg                         >  1:2251299/1‑93 (MQ=255)

                                                                                 tttGCTGCCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATggg                <  1:2250883/101‑1 (MQ=255)

                                                                                   tgctgcCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGaa              <  2:2250891/101‑1 (MQ=255)

                                                                                     ctgcCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgc            >  2:2251302/1‑101 (MQ=255)

                                                                                      tgcCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgcc           <  1:2250840/101‑1 (MQ=255)

                                                                                       gcCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgccg          <  2:2250876/101‑1 (MQ=255)

                                                                                       gcCAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgccg          >  2:2251304/1‑101 (MQ=255)

                                                                                        ccAGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgccgc         >  1:2251305/1‑101 (MQ=255)

                                                                                          aGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTggg                                >  2:2251309/1‑76 (MQ=255)

                                                                                          aGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAgccgc         <  1:2250789/99‑1 (MQ=255)

                                                                                          aGTTGGGCGCTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCct       <  1:2250723/101‑1 (MQ=255)

                                                                                             tGGGCGCTGCTGGCCGTTGGTTTGCGGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCctgct    <  2:2250926/101‑1 (MQ=255)

GAAGAGAAAAATATCCCACAATATTTGCGTTCACACTGGCAGAAAGAGACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCCTGCTGC  >  NC_000913/1339744‑1339939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`