BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,337	T→C	Y61C (TAT→TGT)	sapC ←	putrescine ABC exporter membrane protein SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,337	0	T	C	100.0%	1221.8 / NA	297	Y61C (TAT→TGT)	sapC	putrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (168/129); total (168/129)

Only 100 of 297 total aligned reads displayed.

CGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGC  >  NC_000913/1354239‑1354434

 gCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAAc                                                                                                >  2:2274746/1‑101 (MQ=255)

  cTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAAcc                                                                                               >  1:2274747/1‑101 (MQ=255)

  cTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAAcc                                                                                               <  1:2274305/101‑1 (MQ=255)

  cTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAAcc                                                                                               <  1:2274134/101‑1 (MQ=255)

  cTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAAcc                                                                                               <  1:2274186/101‑1 (MQ=255)

   tCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCg                                                                                              <  2:2274321/101‑1 (MQ=255)

    cAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCga                                                                                             <  1:2273982/101‑1 (MQ=255)

     aGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCgag                                                                                            >  2:2274753/1‑101 (MQ=255)

       tAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGaa                                                                                          <  2:2274316/101‑1 (MQ=255)

       tAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGaa                                                                                          >  1:2274754/1‑101 (MQ=255)

        aaaCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGaaa                                                                                         >  2:2274760/1‑101 (MQ=255)

        aaaCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGaaa                                                                                         >  1:2274759/1‑101 (MQ=255)

         aaCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAAt                                                                                        <  2:2274491/101‑1 (MQ=255)

         aaCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAAt                                                                                        >  1:2274763/1‑101 (MQ=255)

          aCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAAtt                                                                                       <  1:2274375/101‑1 (MQ=255)

           cGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTg                                                                                      <  1:2274054/101‑1 (MQ=255)

            ggCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGc                                                                                     <  2:2274298/101‑1 (MQ=255)

            ggCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGc                                                                                     <  1:2274296/101‑1 (MQ=255)

             gCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCt                                                                                    >  2:2274767/1‑101 (MQ=255)

             gCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCt                                                                                    <  2:2274153/101‑1 (MQ=255)

             gCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCt                                                                                    >  1:2274769/1‑101 (MQ=255)

                tAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGt                                                                                 >  2:2274773/1‑101 (MQ=255)

                 aaaaCATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTg                                                                                   <  2:2274425/98‑1 (MQ=255)

                 aaaaCATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTc                                                                                >  2:2274776/1‑101 (MQ=255)

                    aCATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGAt                                                                             <  2:2274411/101‑1 (MQ=255)

                      aTCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGc                                                                           <  1:2274359/101‑1 (MQ=255)

                      aTCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGc                                                                           >  1:2274780/1‑101 (MQ=255)

                       tCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGcc                                                                          >  2:2274782/1‑101 (MQ=255)

                         gcgTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGt                                                                        >  1:2274785/1‑101 (MQ=255)

                         gcgTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGt                                                                        <  2:2274471/101‑1 (MQ=255)

                         gcgTCAGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGt                                                                        <  1:2274264/101‑1 (MQ=255)

                           gTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCg                                                                         >  1:2274793/1‑98 (MQ=255)

                           gTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTaa                                                                      >  2:2274791/1‑101 (MQ=255)

                           gTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTaa                                                                      >  1:2274789/1‑101 (MQ=255)

                             ccGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAgg                                                                    <  2:2274394/101‑1 (MQ=255)

                             ccGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAgg                                                                    >  2:2274796/1‑101 (MQ=255)

                                  gtcgtcAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCaa                                                               >  1:2274801/1‑101 (MQ=255)

                                  gtcgtcAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCaa                                                               <  2:2274182/101‑1 (MQ=255)

                                   tcgtcAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCaaa                                                              <  1:2274282/101‑1 (MQ=255)

                                    cgtcAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc                                                             >  2:2274803/1‑101 (MQ=255)

                                      tcAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCa                                                           >  2:2274806/1‑101 (MQ=255)

                                       cAGTCCCCAGGAAGAAATAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAg                                                          <  2:2274365/101‑1 (MQ=255)

                                         gTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAgcc                                                        <  2:2274309/101‑1 (MQ=255)

                                         gTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAgcc                                                        <  2:2274278/101‑1 (MQ=255)

                                         gTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAgcc                                                        >  2:2274810/1‑101 (MQ=255)

                                         gTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAgcc                                                        >  1:2274811/1‑101 (MQ=255)

                                            cccAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAgccgcc                                                     >  2:2274815/1‑101 (MQ=255)

                                             ccAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGt                                                                                 <  2:2274056/72‑1 (MQ=255)

                                             ccAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCa                                                    >  2:2274821/1‑101 (MQ=255)

                                             ccAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCa                                                    >  1:2274818/1‑101 (MQ=255)

                                              cAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCaa                                                   <  1:2274336/101‑1 (MQ=255)

                                              cAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCaa                                                   >  1:2274822/1‑101 (MQ=255)

                                               aGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAgccg                                                       <  2:2274430/96‑1 (MQ=255)

                                               aGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCaaa                                                  >  2:2274825/1‑101 (MQ=255)

                                                 gaagaaAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCaaaaa                                                <  1:2274090/101‑1 (MQ=255)

                                                  aagaaagaAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCg                                                                               >  2:2274833/1‑69 (MQ=255)

                                                  aagaaagaAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAAt                                               >  2:2274828/1‑101 (MQ=255)

                                                   agaaagaaACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATa                                              <  1:2274391/101‑1 (MQ=255)

                                                    gaaagaaaCTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATac                                             <  2:2274249/101‑1 (MQ=255)

                                                     aaagaaaCTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATaca                                            >  1:2274835/1‑101 (MQ=255)

                                                       agaaaCTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATAcaca                                          <  2:2274369/101‑1 (MQ=255)

                                                        gaaaCTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAg                                         >  2:2274839/1‑101 (MQ=255)

                                                          aaCTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTa                                       >  2:2274843/1‑101 (MQ=255)

                                                          aaCTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTa                                       <  1:2274418/101‑1 (MQ=255)

                                                          aaCTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTa                                       >  1:2274841/1‑101 (MQ=255)

                                                            cTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAca                                     <  2:2274460/101‑1 (MQ=255)

                                                              tCGCCATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAcagc                                   >  2:2274846/1‑101 (MQ=255)

                                                                  cATAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAcagccagc                               >  1:2274850/1‑101 (MQ=255)

                                                                   aTAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACagccagcc                              <  1:2274226/101‑1 (MQ=255)

                                                                    tAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGccc                             >  2:2274852/1‑101 (MQ=255)

                                                                    tAGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGccc                             <  1:2274448/101‑1 (MQ=255)

                                                                     aGCGCGACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGcccc                            <  1:2274486/101‑1 (MQ=255)

                                                                         cgACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCgcgc                        >  2:2274857/1‑101 (MQ=255)

                                                                         cgACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCgcgc                        >  1:2274858/1‑101 (MQ=255)

                                                                          gACCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCa                       >  1:2274862/1‑101 (MQ=255)

                                                                           aCCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAg                      <  1:2274201/101‑1 (MQ=255)

                                                                           aCCATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAg                      >  2:2274866/1‑101 (MQ=255)

                                                                             cATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGcc                    >  1:2274870/1‑101 (MQ=255)

                                                                             cATGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGcc                    >  2:2274869/1‑101 (MQ=255)

                                                                              aTGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCg                   >  2:2274873/1‑101 (MQ=255)

                                                                              aTGACGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCg                   >  2:2274877/1‑101 (MQ=255)

                                                                                 aCGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTAc                <  1:2274245/101‑1 (MQ=255)

                                                                                 aCGGCGGCAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTAc                >  2:2274880/1‑101 (MQ=255)

                                                                                   ggcggcAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAg              >  2:2274883/1‑101 (MQ=255)

                                                                                   ggcggcAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAg              <  2:2274588/101‑1 (MQ=255)

                                                                                   ggcggcAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAg              <  2:2274673/101‑1 (MQ=255)

                                                                                    gcggcAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAgg             >  1:2274885/1‑101 (MQ=255)

                                                                                     cggcAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGc            <  1:2274519/101‑1 (MQ=255)

                                                                                     cggcAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGc            <  2:2274334/101‑1 (MQ=255)

                                                                                      ggcAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGcc           >  1:2274890/1‑101 (MQ=255)

                                                                                       gcAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCg          >  2:2274892/1‑101 (MQ=255)

                                                                                       gcAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCg          <  1:2274167/101‑1 (MQ=255)

                                                                                        cAATAATTGACAACCGAGAAATTGCTGGTCGATGCCGTAAg                                                                     >  2:2274896/1‑41 (MQ=255)

                                                                                         aataatTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAgg             <  1:2274570/96‑1 (MQ=255)

                                                                                         aataatTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAc        >  1:2274899/1‑101 (MQ=255)

                                                                                           taatTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCa      >  2:2274902/1‑101 (MQ=255)

                                                                                            aatTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTTCAGGCCGACCAt     >  2:2274905/1‑101 (MQ=255)

                                                                                             atTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATc    >  1:2274908/1‑101 (MQ=255)

                                                                                             atTGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATc    >  2:2274910/1‑101 (MQ=255)

                                                                                               tGACAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGc  <  2:2274542/101‑1 (MQ=255)

CGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGC  >  NC_000913/1354239‑1354434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`