Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,408,229 G→A P60P (CCG→CCA)  zntB → Zn(2(+)):H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,408,2290GA99.3% 1112.8 / ‑4.3 270P60P (CCG→CCA) zntBZn(2(+)):H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (139/129);  total (141/129)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

CCGCTGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTA  >  NC_000913/1408131‑1408326
                                                                                                  |                                                                                                 
ccGCTGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCact                                                                                                 >  1:2365156/1‑101 (MQ=255)
 cGCTGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCactg                                                                                                <  1:2364209/101‑1 (MQ=255)
 cGCTGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCactg                                                                                                >  1:2365159/1‑101 (MQ=255)
  gCTGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCactgc                                                                                               >  1:2365164/1‑101 (MQ=255)
   cTGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCactgct                                                                                              <  2:2364756/101‑1 (MQ=255)
    tGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCtt                                                                                             <  1:2364647/101‑1 (MQ=255)
    tGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCtt                                                                                             <  2:2364406/101‑1 (MQ=255)
    tGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCtt                                                                                             >  2:2365169/1‑101 (MQ=255)
    tGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCtt                                                                                             <  1:2364711/101‑1 (MQ=255)
     ggAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCGCCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTc                                                                                            <  2:2364822/101‑1 (MQ=255)
     ggAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCact                                                                                                 >  2:2365172/1‑96 (MQ=255)
     ggAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTc                                                                                            <  2:2364548/101‑1 (MQ=255)
      gAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTcc                                                                                           <  2:2364611/101‑1 (MQ=255)
      gAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTcc                                                                                           >  1:2365174/1‑101 (MQ=255)
        aaaTACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCa                                                                                         >  2:2365178/1‑101 (MQ=255)
         aaTACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCaa                                                                                        >  1:2365180/1‑101 (MQ=255)
           tACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATa                                                                                      >  2:2365185/1‑101 (MQ=255)
           tACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATa                                                                                      <  2:2364772/101‑1 (MQ=255)
             cAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAac                                                                                    <  2:2364791/101‑1 (MQ=255)
             cAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAac                                                                                    >  1:2365187/1‑101 (MQ=255)
              aGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAacg                                                                                   <  2:2364831/101‑1 (MQ=255)
               gATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAacgt                                                                                  >  2:2365189/1‑101 (MQ=255)
               gATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAacgt                                                                                  <  1:2364616/101‑1 (MQ=255)
                  gtgATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAacgtacg                                                                               >  1:2365193/1‑101 (MQ=255)
                  gtgATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAacgtacg                                                                               <  1:2364696/101‑1 (MQ=255)
                   tgattgatGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAacgtacgt                                                                              <  2:2364710/101‑1 (MQ=255)
                    gattgatGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTg                                                                             <  1:2364726/101‑1 (MQ=255)
                     attgatGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGa                                                                            >  2:2365197/1‑101 (MQ=255)
                      ttgatGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGAt                                                                           <  1:2364861/101‑1 (MQ=255)
                      ttgatGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGAt                                                                           >  1:2365199/1‑101 (MQ=255)
                       tgatgaAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATg                                                                          >  1:2365201/1‑101 (MQ=255)
                           gaAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCt                                                                      <  2:2364490/101‑1 (MQ=255)
                           gaAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCt                                                                      <  1:2364740/101‑1 (MQ=255)
                           gaAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCt                                                                      >  1:2365206/1‑101 (MQ=255)
                           gaAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCt                                                                      >  2:2365205/1‑101 (MQ=255)
                            aaGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATg                                                                          >  2:2365210/1‑96 (MQ=255)
                              gcgcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTggc                                                                   <  2:2364947/101‑1 (MQ=255)
                              gcgcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTggc                                                                   <  2:2364876/101‑1 (MQ=255)
                              gcgcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTggc                                                                   <  1:2364636/101‑1 (MQ=255)
                                gcATCCCTGTTGGCTCCACCTTAATTATGTACACCTTGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTggcgg                                                                 <  2:2364524/101‑1 (MQ=255)
                                gcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTggcgg                                                                 >  1:2365214/1‑101 (MQ=255)
                                gcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTggcgg                                                                 >  2:2365217/1‑101 (MQ=255)
                                  aTCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTggcgggc                                                               >  1:2365218/1‑101 (MQ=255)
                                  aTCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTggcgggc                                                               <  1:2364804/101‑1 (MQ=255)
                                    cccTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCga                                                             >  1:2365222/1‑101 (MQ=255)
                                    cccTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCga                                                             <  2:2364817/101‑1 (MQ=255)
                                      cTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCga                                                             >  2:2365225/1‑99 (MQ=255)
                                       tGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGagag                                                          <  2:2364686/101‑1 (MQ=255)
                                       tGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGagag                                                          >  1:2365227/1‑101 (MQ=255)
                                          tGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCAc                                                       >  2:2365230/1‑101 (MQ=255)
                                            gCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTggcggg                                                                >  2:2365235/1‑90 (MQ=255)
                                             cTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCAcgcg                                                    >  1:2365236/1‑101 (MQ=255)
                                               ccACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAacgt                                                                                  >  2:2365240/1‑69 (MQ=255)
                                                 aCCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTccc                                                >  1:2365243/1‑101 (MQ=255)
                                                  ccTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTcccc                                               >  2:2365245/1‑101 (MQ=255)
                                                  ccTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTcccc                                               <  1:2364921/101‑1 (MQ=255)
                                                     tAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAg                                            >  1:2365248/1‑101 (MQ=255)
                                                     tAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAg                                            <  2:2364912/101‑1 (MQ=255)
                                                       aTTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTc                                          <  2:2365087/101‑1 (MQ=255)
                                                        ttATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCa                                         >  2:2365254/1‑101 (MQ=255)
                                                        ttATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCa                                         <  1:2364326/101‑1 (MQ=255)
                                                        ttATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCa                                         >  1:2365255/1‑101 (MQ=255)
                                                          aTGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGc                                       <  2:2364642/101‑1 (MQ=255)
                                                             tACACCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGt                                    >  2:2365259/1‑101 (MQ=255)
                                                              acacCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGtc                                   >  1:2365260/1‑101 (MQ=255)
                                                               cacCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGtct                                  >  1:2365264/1‑101 (MQ=255)
                                                                acCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGtctc                                 <  1:2364689/101‑1 (MQ=255)
                                                                acCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGtctc                                 <  1:2364660/101‑1 (MQ=255)
                                                                acCATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGtctc                                 <  1:2364672/101‑1 (MQ=255)
                                                                 ccATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCg                                >  2:2365265/1‑101 (MQ=255)
                                                                  cATGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCgag                                                            <  2:2364776/72‑1 (MQ=255)
                                                                   aTGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGt                              >  2:2365268/1‑101 (MQ=255)
                                                                   aTGATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGt                              <  2:2364901/101‑1 (MQ=255)
                                                                     gATAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGa                            >  2:2365271/1‑101 (MQ=255)
                                                                       tAGCGCCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAg                          >  2:2365274/1‑101 (MQ=255)
                                                                       tAGCGCCAAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATgcg                                                                        >  2:2365277/1‑55 (MQ=255)
                                                                          cgcCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCa                       <  2:2364894/101‑1 (MQ=255)
                                                                          cgcCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCa                       <  1:2364755/101‑1 (MQ=255)
                                                                           gcCCAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCAc                      <  1:2364494/101‑1 (MQ=255)
                                                                            cccAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACg                     <  2:2364745/101‑1 (MQ=255)
                                                                             ccAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGc                    <  1:2364874/101‑1 (MQ=255)
                                                                             ccAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGc                    >  1:2365282/1‑101 (MQ=255)
                                                                              cAATGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGc                        <  1:2364997/96‑1 (MQ=255)
                                                                               aaTGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATgcgc                                                                       >  2:2365287/1‑48 (MQ=255)
                                                                               aaTGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTg                  >  1:2365285/1‑101 (MQ=255)
                                                                                aTGGCTGGCGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGa                 <  1:2364890/101‑1 (MQ=255)
                                                                                 tggctggcGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGAt                >  1:2365290/1‑101 (MQ=255)
                                                                                  ggctggcGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGAtt               <  1:2364788/101‑1 (MQ=255)
                                                                                     tggcGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGAt                >  2:2365296/1‑97 (MQ=255)
                                                                                     tggcGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACa            >  1:2365294/1‑101 (MQ=255)
                                                                                      ggcGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACAt           <  1:2364836/101‑1 (MQ=255)
                                                                                       gcGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTAc             <  1:2364811/98‑1 (MQ=255)
                                                                                        cGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCgag                                                            >  2:2365300/1‑50 (MQ=255)
                                                                                        cGACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTg         <  2:2365162/101‑1 (MQ=255)
                                                                                         gACAACACCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTgc        >  1:2365301/1‑101 (MQ=255)
                                                                                           caacaCCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTgcgc      >  1:2365306/1‑101 (MQ=255)
                                                                                            aacaCCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTg                             <  2:2364860/77‑1 (MQ=255)
                                                                                            aacaCCACTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCt     >  2:2365307/1‑101 (MQ=255)
                                                                                              caccacTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGt   <  2:2364999/101‑1 (MQ=255)
                                                                                              caccacTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGt   <  2:2364671/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCGCTGGAAAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTA  >  NC_000913/1408131‑1408326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑