Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,699,674 T→C V107A (GTA→GCA)  malX → PTS enzyme IIBC component MalX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,699,6740TC99.7% 1315.3 / ‑4.3 320V107A (GTA→GCA) malXPTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (158/161);  total (159/161)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 320 total aligned reads displayed.

AGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTT  >  NC_000913/1699577‑1699771
                                                                                                 |                                                                                                 
aGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAAt                                                                                                <  1:2853935/101‑1 (MQ=255)
 gTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATg                                                                                               <  2:2853833/101‑1 (MQ=255)
 gTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATg                                                                                               <  2:2853959/101‑1 (MQ=255)
 gTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATg                                                                                               >  2:2854384/1‑101 (MQ=255)
   ttCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGaa                                                                                             >  1:2854385/1‑101 (MQ=255)
   ttCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGaa                                                                                             <  1:2853914/101‑1 (MQ=255)
    tCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAAc                                                                                            <  1:2854082/101‑1 (MQ=255)
     cctgcctgTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAAcc                                                                                           <  2:2853628/101‑1 (MQ=255)
      ctgcctgTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCt                                                                                          >  1:2854389/1‑101 (MQ=255)
       tgcctgTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTc                                                                                         >  2:2854391/1‑101 (MQ=255)
       tgcctgTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTc                                                                                         <  2:2853856/101‑1 (MQ=255)
        gcctgTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTcg                                                                                        <  2:2853977/101‑1 (MQ=255)
        gcctgTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTcg                                                                                        <  2:2853942/101‑1 (MQ=255)
          ctgTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTcgcg                                                                                      <  1:2853753/101‑1 (MQ=255)
           tgTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCgg                                                                                     <  2:2854011/101‑1 (MQ=255)
            gTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGt                                                                                    <  2:2853898/101‑1 (MQ=255)
              cATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTaa                                                                                  >  2:2854398/1‑101 (MQ=255)
               aTGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTaaa                                                                                 >  1:2854399/1‑101 (MQ=255)
                   tCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTc                                                                             >  1:2854404/1‑101 (MQ=255)
                     tGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTg                                                                           >  2:2854408/1‑101 (MQ=255)
                      gTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTc                                                                             <  1:2853952/98‑1 (MQ=255)
                      gTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTgg                                                                          >  1:2854409/1‑101 (MQ=255)
                         tCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTg                                                                       >  1:2854414/1‑101 (MQ=255)
                          cGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGa                                                                      >  2:2854417/1‑101 (MQ=255)
                           gCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGAc                                                                     >  1:2854420/1‑101 (MQ=255)
                           gCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGAc                                                                     >  2:2854422/1‑101 (MQ=255)
                            ccATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGAcc                                                                    <  1:2853801/101‑1 (MQ=255)
                             cATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCa                                                                   >  2:2854425/1‑101 (MQ=255)
                              aTCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCaa                                                                  <  2:2854151/101‑1 (MQ=255)
                              aTCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCaa                                                                  <  1:2853706/101‑1 (MQ=255)
                              aTCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCaa                                                                  >  1:2854431/1‑101 (MQ=255)
                                cccGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATa                                                                >  2:2854434/1‑101 (MQ=255)
                                 ccGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATaa                                                               <  1:2854176/101‑1 (MQ=255)
                                 ccGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATaa                                                               <  2:2853846/101‑1 (MQ=255)
                                 ccGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATaa                                                               <  2:2854076/101‑1 (MQ=255)
                                 ccGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATaa                                                               <  1:2854090/101‑1 (MQ=255)
                                  cGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATaaa                                                              >  1:2854439/1‑101 (MQ=255)
                                   gCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGAcc                                                                    <  1:2853849/94‑1 (MQ=255)
                                   gCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAAt                                                                 <  2:2853937/97‑1 (MQ=255)
                                   gCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAg                                                             >  2:2854441/1‑101 (MQ=255)
                                    cTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAgg                                                            >  1:2854444/1‑101 (MQ=255)
                                     tGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGc                                                           <  1:2853981/101‑1 (MQ=255)
                                     tGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGc                                                           >  2:2854447/1‑101 (MQ=255)
                                       ggCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCAt                                                         >  1:2854450/1‑101 (MQ=255)
                                       ggCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCAt                                                         <  1:2854059/101‑1 (MQ=255)
                                         ccTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTc                                                       >  2:2854453/1‑101 (MQ=255)
                                          cTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCt                                                      >  1:2854455/1‑101 (MQ=255)
                                            ggCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGc                                                    <  2:2854170/101‑1 (MQ=255)
                                              cACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCa                                                  >  1:2854460/1‑101 (MQ=255)
                                               aCGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCaa                                                 >  2:2854463/1‑101 (MQ=255)
                                               aCGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCaa                                                 <  2:2854129/101‑1 (MQ=255)
                                                cgcgAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAAc                                                >  1:2854465/1‑101 (MQ=255)
                                                cgcgAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAAc                                                <  2:2854097/101‑1 (MQ=255)
                                                  cgAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCa                                              <  1:2854040/101‑1 (MQ=255)
                                                  cgAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCa                                              >  1:2854470/1‑101 (MQ=255)
                                                   gAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCAc                                             <  1:2854018/101‑1 (MQ=255)
                                                   gAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCAc                                             >  2:2854471/1‑101 (MQ=255)
                                                   gAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCAc                                             <  1:2854101/101‑1 (MQ=255)
                                                   gAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCAc                                             >  1:2854474/1‑101 (MQ=255)
                                                    aaaaTAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACg                                            <  2:2853878/101‑1 (MQ=255)
                                                     aaaTAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACgg                                           <  2:2853820/101‑1 (MQ=255)
                                                     aaaTAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACgg                                           <  2:2854084/101‑1 (MQ=255)
                                                     aaaTAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACgg                                           >  1:2854477/1‑101 (MQ=255)
                                                      aaTAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGa                                          <  2:2853760/101‑1 (MQ=255)
                                                      aaTAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGa                                          <  2:2854022/101‑1 (MQ=255)
                                                       aTAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGAt                                         <  2:2853710/101‑1 (MQ=255)
                                                       aTAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGAt                                         <  2:2853685/101‑1 (MQ=255)
                                                        tAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATg                                        <  2:2853973/101‑1 (MQ=255)
                                                        tAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATg                                        >  1:2854482/1‑101 (MQ=255)
                                                           aGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCcg                                     >  2:2854486/1‑101 (MQ=255)
                                                             gCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCcgcg                                   <  1:2854117/101‑1 (MQ=255)
                                                               gTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGc                                                           <  1:2853905/75‑1 (MQ=255)
                                                               gTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGt                                 >  1:2854488/1‑101 (MQ=255)
                                                               gTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGt                                 >  2:2854491/1‑101 (MQ=255)
                                                                  gCGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCt                              >  2:2854495/1‑101 (MQ=255)
                                                                   cGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTg                             >  2:2854499/1‑101 (MQ=255)
                                                                   cGGCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCAGCGGTTCTg                             <  1:2853962/101‑1 (MQ=255)
                                                                    ggCATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGa                            >  1:2854500/1‑101 (MQ=255)
                                                                      cATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGaaa                          >  1:2854504/1‑101 (MQ=255)
                                                                      cATTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGaaa                          <  1:2854130/101‑1 (MQ=255)
                                                                       aTTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAg                         <  2:2853929/101‑1 (MQ=255)
                                                                       aTTCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAg                         >  1:2854507/1‑101 (MQ=255)
                                                                        ttCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGc                        >  1:2854512/1‑101 (MQ=255)
                                                                         tCGCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGcc                       >  2:2854513/1‑101 (MQ=255)
                                                                           gCTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCaa                     >  1:2854518/1‑101 (MQ=255)
                                                                            cTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAAt                    >  2:2854520/1‑101 (MQ=255)
                                                                            cTGGCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAAt                    <  1:2853305/101‑1 (MQ=255)
                                                                              ggCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATaa                  >  1:2854524/1‑101 (MQ=255)
                                                                              ggCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATaa                  <  2:2854214/101‑1 (MQ=255)
                                                                               gCTTCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAAc                 <  1:2854280/101‑1 (MQ=255)
                                                                                 ttCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACAt               <  1:2854006/101‑1 (MQ=255)
                                                                                 ttCATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACAt               >  1:2854527/1‑101 (MQ=255)
                                                                                   cATCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATcc             >  2:2854529/1‑101 (MQ=255)
                                                                                    aTCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCa            <  2:2853739/101‑1 (MQ=255)
                                                                                    aTCGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCa            >  2:2854533/1‑101 (MQ=255)
                                                                                      cGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCaga          >  2:2854537/1‑101 (MQ=255)
                                                                                      cGGTTATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCaga          >  1:2854538/1‑101 (MQ=255)
                                                                                         ttATGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCa       >  2:2854541/1‑101 (MQ=255)
                                                                                           aTGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCAtt     <  2:2854037/101‑1 (MQ=255)
                                                                                            tGCGGCAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTc    <  1:2853873/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
AGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTT  >  NC_000913/1699577‑1699771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑