BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,021,565	T→C	V21A (GTG→GCG)	fliO →	flagellar biosynthesis protein FliO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,021,565	0	T	C	100.0%	1336.1 / NA	320	V21A (GTG→GCG)	fliO	flagellar biosynthesis protein FliO
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (180/140); total (180/140)

Only 100 of 320 total aligned reads displayed.

TCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGT  >  NC_000913/2021468‑2021662

tCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAg                                                                                                <  1:3383851/101‑1 (MQ=255)

  cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGa                                                                                                 <  1:3384211/98‑1 (MQ=255)

  cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAgcg                                                                                              >  2:3384349/1‑101 (MQ=255)

  cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAgcg                                                                                              >  1:3384351/1‑101 (MQ=255)

    tCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAgcggc                                                                                            >  1:3384355/1‑101 (MQ=255)

     cTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAgcggcg                                                                                           >  2:3384359/1‑101 (MQ=255)

       gAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCa                                                                                         >  2:3384363/1‑101 (MQ=255)

          cGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTc                                                                                      >  1:3384367/1‑101 (MQ=255)

          cGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTc                                                                                      >  2:3384366/1‑101 (MQ=255)

           gAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCa                                                                                     >  2:3384370/1‑101 (MQ=255)

            aaTGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCAt                                                                                    >  1:3384373/1‑101 (MQ=255)

              tGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCg                                                                                  >  2:3384377/1‑101 (MQ=255)

                 gccgccTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCa                                                                               >  1:3384382/1‑101 (MQ=255)

                   cgccTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCatt                                                                             <  1:3384107/101‑1 (MQ=255)

                   cgccTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCatt                                                                             >  2:3384386/1‑101 (MQ=255)

                    gccTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCatta                                                                            <  1:3383783/101‑1 (MQ=255)

                     ccTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCattat                                                                           <  1:3383899/101‑1 (MQ=255)

                      cTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCattatt                                                                          >  1:3384389/1‑101 (MQ=255)

                       tGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTg                                                                         >  2:3384391/1‑101 (MQ=255)

                           ccGTTAGTGATGAATAACCACGCTACTGTGTAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCt                                                                     >  2:3384395/1‑101 (MQ=255)

                           ccGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCt                                                                     <  2:3384048/101‑1 (MQ=255)

                            cGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTg                                                                    <  1:3383967/101‑1 (MQ=255)

                            cGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTg                                                                    <  1:3383570/101‑1 (MQ=255)

                             gTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGa                                                                   >  1:3384401/1‑101 (MQ=255)

                             gTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGa                                                                   >  2:3384399/1‑101 (MQ=255)

                              ttAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCt                                                                     <  2:3383922/98‑1 (MQ=255)

                              ttAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGAt                                                                  >  1:3384404/1‑101 (MQ=255)

                              ttAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGAt                                                                  <  1:3384038/101‑1 (MQ=255)

                              ttAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGAt                                                                  <  1:3383985/101‑1 (MQ=255)

                                 gTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCt                                                               <  1:3384070/101‑1 (MQ=255)

                                 gTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCt                                                               <  1:3384006/101‑1 (MQ=255)

                                  tgatgaATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTc                                                              >  2:3384409/1‑101 (MQ=255)

                                   gatgaATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCg                                                             >  1:3384411/1‑101 (MQ=255)

                                    atgaATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgc                                                            <  2:3384312/101‑1 (MQ=255)

                                     tgaATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgct                                                           <  1:3383972/101‑1 (MQ=255)

                                      gaATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgctg                                                          >  1:3384415/1‑101 (MQ=255)

                                      gaATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgctg                                                          <  2:3383960/101‑1 (MQ=255)

                                        aTAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgctgct                                                        >  1:3384419/1‑101 (MQ=255)

                                         tAACCACGGTACTGTGCAATCTTCCGAGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGctgctg                                                       >  1:3384423/1‑101 (MQ=255)

                                          aaCCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCtgctgc                                                      >  2:3384424/1‑101 (MQ=255)

                                           aCCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATc                                                                                   <  2:3383877/71‑1 (MQ=255)

                                             cACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctg                                                   <  1:3384030/101‑1 (MQ=255)

                                             cACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctg                                                   <  2:3383928/101‑1 (MQ=255)

                                             cACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctg                                                   >  1:3384430/1‑101 (MQ=255)

                                             cACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctg                                                   >  2:3384428/1‑101 (MQ=255)

                                                gCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctggct                                                <  2:3384122/101‑1 (MQ=255)

                                                gCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctggct                                                >  1:3384434/1‑101 (MQ=255)

                                                 cTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctggctg                                               >  2:3384436/1‑101 (MQ=255)

                                                 cTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctggctg                                               <  2:3384016/101‑1 (MQ=255)

                                                  tACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctggctgg                                              <  2:3384085/101‑1 (MQ=255)

                                                  tACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctggctgg                                              >  1:3384440/1‑101 (MQ=255)

                                                   aCTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGt                                             >  2:3384445/1‑101 (MQ=255)

                                                    cTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAgc                                                                                               >  2:3384449/1‑50 (MQ=255)

                                                    cTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATcc                                                                <  2:3383790/81‑1 (MQ=255)

                                                    cTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTa                                            >  1:3384448/1‑101 (MQ=255)

                                                      gtgCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTaaa                                          >  1:3384454/1‑101 (MQ=255)

                                                       tgCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTaaaa                                         <  1:3384077/101‑1 (MQ=255)

                                                        gCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAAc                                        <  2:3384034/101‑1 (MQ=255)

                                                         cAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACg                                       <  2:3384061/101‑1 (MQ=255)

                                                          aaTCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACgg                                      >  2:3384457/1‑101 (MQ=255)

                                                          aaTCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACgg                                      <  2:3383944/101‑1 (MQ=255)

                                                           aTCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCt                                                                     >  1:3384460/1‑69 (MQ=255)

                                                            tCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGtt                                    >  1:3384463/1‑101 (MQ=255)

                                                             cTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCAc                                                                                        <  2:3384053/48‑1 (MQ=255)

                                                             cTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTg                                   >  2:3384465/1‑101 (MQ=255)

                                                               tCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTggg                                 <  2:3383916/101‑1 (MQ=255)

                                                               tCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTggg                                 >  2:3384471/1‑101 (MQ=255)

                                                                ccGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGa                                <  1:3384199/101‑1 (MQ=255)

                                                                ccGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGa                                <  2:3383830/101‑1 (MQ=255)

                                                                ccGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGa                                >  1:3384472/1‑101 (MQ=255)

                                                                 cgcgCCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGAt                               <  1:3384124/101‑1 (MQ=255)

                                                                  gcgcCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGAtt                              >  2:3384476/1‑101 (MQ=255)

                                                                   cgcCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGAttt                             >  1:3384481/1‑101 (MQ=255)

                                                                    gcCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTg                            >  2:3384482/1‑101 (MQ=255)

                                                                    gcCGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTg                            >  2:3384488/1‑101 (MQ=255)

                                                                     ccGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGc                           >  1:3384489/1‑101 (MQ=255)

                                                                      cGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGAttt                             >  2:3384496/1‑98 (MQ=255)

                                                                      cGGTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGcc                          >  1:3384494/1‑101 (MQ=255)

                                                                       ggTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGccc                         <  1:3383935/101‑1 (MQ=255)

                                                                       ggTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGccc                         >  1:3384498/1‑101 (MQ=255)

                                                                        gTTTCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGcccc                        >  1:3384502/1‑101 (MQ=255)

                                                                         tttCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGAttt                             <  2:3383845/95‑1 (MQ=255)

                                                                         tttCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCt                       <  2:3384163/101‑1 (MQ=255)

                                                                          ttCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTa                      >  1:3384507/1‑101 (MQ=255)

                                                                           tCTGCTGCGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTaa                     >  2:3384508/1‑101 (MQ=255)

                                                                             tgctgcGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAAc                   <  1:3384094/101‑1 (MQ=255)

                                                                              gctgcGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACg                  <  2:3384002/101‑1 (MQ=255)

                                                                                tgcGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                <  1:3383821/101‑1 (MQ=255)

                                                                                tgcGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                >  1:3384512/1‑101 (MQ=255)

                                                                                tgcGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                <  2:3384011/101‑1 (MQ=255)

                                                                                tgcGCCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCa                <  2:3384090/101‑1 (MQ=255)

                                                                                  cgcCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTaa                     >  2:3384515/1‑94 (MQ=255)

                                                                                   gcCACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTg             >  1:3384519/1‑101 (MQ=255)

                                                                                    ccACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTgg            <  2:3384149/101‑1 (MQ=255)

                                                                                     cACTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGc           >  2:3384520/1‑101 (MQ=255)

                                                                                      aCTGCTGCAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCg          <  2:3383739/101‑1 (MQ=255)

                                                                                        tgctgcAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGtt        <  1:3383941/101‑1 (MQ=255)

                                                                                         gctgcAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTa       <  2:3383839/101‑1 (MQ=255)

                                                                                          ctgcAGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTaa      >  1:3384525/1‑101 (MQ=255)

                                                                                              aGGCGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGt  >  1:3384529/1‑101 (MQ=255)

TCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAACGGTTGGGATTTGCCCCTAAACGCACTGGCGTTAACGGT  >  NC_000913/2021468‑2021662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`