BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,465,498	G→A	A66A (GCG→GCA)	yfdC →	inner membrane protein YfdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,465,498	0	G	A	100.0%	1397.1 / NA	320	A66A (GCG→GCA)	yfdC	inner membrane protein YfdC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (134/186); total (134/186)

Only 100 of 342 total aligned reads displayed.

CCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATA  >  NC_000913/2465400‑2465595

 cctcccGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGt                                                                                                >  2:4132384/1‑101 (MQ=255)

 cctcccGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGt                                                                                                >  1:4132386/1‑101 (MQ=255)

  ctcccGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTc                                                                                               <  1:4131557/101‑1 (MQ=255)

  ctcccGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTc                                                                                               >  1:4132389/1‑101 (MQ=255)

   tcccGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCt                                                                                              >  1:4132394/1‑101 (MQ=255)

     ccGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGt                                                                                            >  2:4132398/1‑101 (MQ=255)

        ggCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCg                                                                                          >  2:4132402/1‑100 (MQ=255)

        ggCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGa                                                                                         <  2:4131953/101‑1 (MQ=255)

        ggCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGa                                                                                         <  1:4131951/101‑1 (MQ=255)

           gATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATgg                                                                                      <  1:4131572/101‑1 (MQ=255)

           gATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATgg                                                                                      >  2:4132408/1‑101 (MQ=255)

           gATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATgg                                                                                      <  2:4131465/101‑1 (MQ=255)

           gATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATgg                                                                                      >  1:4132407/1‑101 (MQ=255)

               gCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATggg                                                                                     >  2:4132415/1‑98 (MQ=255)

               gCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGgcgc                                                                                  >  1:4132413/1‑101 (MQ=255)

                 aaTTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATggg                                                                                     >  2:4132422/1‑96 (MQ=255)

                 aaTTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCtt                                                                                >  1:4132420/1‑101 (MQ=255)

                   ttCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCg                                                                              <  1:4131810/101‑1 (MQ=255)

                   ttCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCg                                                                              >  1:4132424/1‑101 (MQ=255)

                     cATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGtt                                                                            <  2:4131891/101‑1 (MQ=255)

                         aGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTg                                                                        >  2:4132430/1‑101 (MQ=255)

                         aGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTg                                                                        <  2:4131923/101‑1 (MQ=255)

                          gCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTgg                                                                       <  1:4131989/101‑1 (MQ=255)

                          gCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTgg                                                                       <  1:4131723/101‑1 (MQ=255)

                          gCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTgg                                                                       <  1:4131455/101‑1 (MQ=255)

                          gCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTgg                                                                       >  1:4132432/1‑101 (MQ=255)

                           cATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGc                                                                      <  1:4131878/101‑1 (MQ=255)

                              atCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCaaa                                                                   <  2:4131846/101‑1 (MQ=255)

                              atCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCaaa                                                                   <  2:4132055/101‑1 (MQ=255)

                               tCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCaaaa                                                                  <  1:4131829/101‑1 (MQ=255)

                                ccGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAg                                                                 >  2:4132437/1‑101 (MQ=255)

                                  gCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAggg                                                               >  1:4132439/1‑101 (MQ=255)

                                    cAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGat                                                             >  2:4132444/1‑101 (MQ=255)

                                     aGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGata                                                            <  2:4131937/101‑1 (MQ=255)

                                     aGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGata                                                            >  1:4132449/1‑101 (MQ=255)

                                     aGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGata                                                            <  1:4132280/101‑1 (MQ=255)

                                        aTGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATAttt                                                         >  2:4132453/1‑101 (MQ=255)

                                        aTGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATAttt                                                         >  1:4132454/1‑101 (MQ=255)

                                         tGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTc                                                        <  2:4132089/101‑1 (MQ=255)

                                         tGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTc                                                        <  1:4132065/101‑1 (MQ=255)

                                         tGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTc                                                        <  1:4131639/101‑1 (MQ=255)

                                            tGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAg                                                     <  2:4132136/101‑1 (MQ=255)

                                             gAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGt                                                    <  1:4131873/101‑1 (MQ=255)

                                             gAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGt                                                    <  1:4131910/101‑1 (MQ=255)

                                             gAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGt                                                    >  1:4132460/1‑101 (MQ=255)

                                              aaaaaGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTc                                                   >  2:4132461/1‑101 (MQ=255)

                                               aaaaGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCg                                                  <  2:4131749/101‑1 (MQ=255)

                                                aaaGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGa                                                 >  2:4132465/1‑101 (MQ=255)

                                                aaaGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGa                                                 <  1:4131748/101‑1 (MQ=255)

                                                   gagCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACt                                              <  1:4132091/101‑1 (MQ=255)

                                                   gagCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACt                                              <  2:4131716/101‑1 (MQ=255)

                                                    agCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATggg                                                                                     >  1:4132470/1‑61 (MQ=255)

                                                      cTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGa                                           >  2:4132474/1‑101 (MQ=255)

                                                       tGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGaa                                          >  1:4132477/1‑101 (MQ=255)

                                                        ggAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAg                                         >  2:4132478/1‑101 (MQ=255)

                                                          aaCGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGgt                                       <  1:4131852/101‑1 (MQ=255)

                                                           aCGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGa                                                              <  2:4132111/77‑1 (MQ=255)

                                                           aCGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGgtg                                      <  1:4131884/101‑1 (MQ=255)

                                                            cgcgACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGgtgt                                     <  2:4131821/101‑1 (MQ=255)

                                                            cgcgACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGgtgt                                     <  2:4132023/101‑1 (MQ=255)

                                                              cgACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGGTGGgggc                                                                                  >  1:4132486/1‑54 (MQ=255)

                                                              cgACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAgg                                        >  2:4132485/1‑96 (MQ=255)

                                                               gACGCAATGGCGCTATTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAg                                                     <  1:4131683/82‑1 (MQ=255)

                                                               gACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCtt                                                                                <  2:4131834/55‑1 (MQ=255)

                                                               gACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAgg                                        >  2:4132489/1‑95 (MQ=255)

                                                                 cGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCgg                                <  2:4131794/101‑1 (MQ=255)

                                                                 cGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCgg                                <  1:4131798/101‑1 (MQ=255)

                                                                 cGCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCgg                                <  1:4132174/101‑1 (MQ=255)

                                                                  gCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTTGAAGGTGTGCCggg                               <  2:4131614/101‑1 (MQ=255)

                                                                  gCAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCggg                               <  1:4132018/101‑1 (MQ=255)

                                                                   cAATGGCGCTACTGTGGTCAGCCATTGCGGCAGGGCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATAttt                                                         >  2:4132493/1‑74 (MQ=255)

                                                                    aaTGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGgtg                                      >  2:4132499/1‑92 (MQ=255)

                                                                    aaTGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCa                             >  1:4132497/1‑101 (MQ=255)

                                                                     aTGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCa                             <  1:4132155/100‑1 (MQ=255)

                                                                      tGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGc                           <  1:4132132/101‑1 (MQ=255)

                                                                      tGGCGCTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGc                           <  1:4131963/101‑1 (MQ=255)

                                                                         cgcTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATTTTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGcttc                        <  1:4132007/101‑1 (MQ=255)

                                                                         cgcTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCg                                 <  1:4131971/92‑1 (MQ=255)

                                                                          gcTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGcttct                       <  1:4132084/101‑1 (MQ=255)

                                                                           cTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGcttctt                      >  2:4132505/1‑101 (MQ=255)

                                                                           cTACTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGcttctt                      <  2:4131904/101‑1 (MQ=255)

                                                                             aCTGTGGTCAGCCATTGCGGCAGGTTTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGctt                         <  2:4131972/96‑1 (MQ=255)

                                                                             aCTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGc                    >  1:4132507/1‑101 (MQ=255)

                                                                             aCTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGc                    <  1:4131981/101‑1 (MQ=255)

                                                                              cTGTGGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCt                   >  2:4132511/1‑101 (MQ=255)

                                                                                gtgGTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTgg                 <  1:4132033/101‑1 (MQ=255)

                                                                                  ggTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGgag               >  1:4132514/1‑101 (MQ=255)

                                                                                   gTCAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGgaga              <  2:4131871/101‑1 (MQ=255)

                                                                                     cAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTgg                 >  2:4132518/1‑96 (MQ=255)

                                                                                     cAGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAAt            <  2:4132447/101‑1 (MQ=255)

                                                                                      aGCCATTGCGGCAGGTCTGTCGATGGGGGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAAtc           >  1:4132519/1‑101 (MQ=255)

                                                                                      aGCCATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAAtc           <  1:4131901/101‑1 (MQ=255)

                                                                                         cATTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCg        <  2:4131993/101‑1 (MQ=255)

                                                                                          aTTGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCgg       <  2:4132012/101‑1 (MQ=255)

                                                                                           ttGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGt      <  2:4131685/101‑1 (MQ=255)

                                                                                            tGCGGCAGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGtt     <  2:4131948/101‑1 (MQ=255)

                                                                                             gcggcaGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTta    <  2:4132194/101‑1 (MQ=255)

                                                                                              cggcagGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATAttt                                                         <  1:4131766/47‑1 (MQ=255)

                                                                                              cggcagGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGttgt   <  1:4131943/101‑3 (MQ=255)

                                                                                               ggcagGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGga                <  1:4132049/87‑1 (MQ=255)

CCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATA  >  NC_000913/2465400‑2465595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`