BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,821,424	G→C	G196G (GGC→GGG)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,821,424	0	G	C	100.0%	871.7 / NA	214	G196G (GGC→GGG)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (96/118); total (96/118)

Only 100 of 222 total aligned reads displayed.

GGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATG  >  NC_000913/2821326‑2821521

ggTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGccc                                                                                                 >  2:4730170/1‑101 (MQ=255)

   tCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccccc                                                                                              <  2:4729689/101‑1 (MQ=255)

   tCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccccc                                                                                              <  1:4729833/101‑1 (MQ=255)

     cATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccccccc                                                                                            >  2:4730172/1‑101 (MQ=255)

     cATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccccccc                                                                                            >  1:4730173/1‑101 (MQ=255)

      aTCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCa                                                                                           <  2:4729813/101‑1 (MQ=255)

          tGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtg                                                                                       >  2:4730175/1‑101 (MQ=255)

           gCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgt                                                                                      <  1:4729653/101‑1 (MQ=255)

            ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgtg                                                                                     <  1:4729610/101‑1 (MQ=255)

             cATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTgg                                                                                    >  2:4730179/1‑101 (MQ=255)

             cATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTgg                                                                                    <  1:4729633/101‑1 (MQ=255)

              aTCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGt                                                                                   <  2:4729913/101‑1 (MQ=255)

                cGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgtg                                                                                     <  1:4729726/97‑1 (MQ=255)

                cGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCg                                                                                 <  1:4729944/101‑1 (MQ=255)

                cGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGACg                                                                                 <  2:4729786/101‑1 (MQ=255)

                 ggCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGc                                                                                >  2:4730182/1‑101 (MQ=255)

                  gCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGcc                                                                               <  2:4729850/101‑1 (MQ=255)

                   ccTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCg                                                                              <  2:4729853/101‑1 (MQ=255)

                   ccTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCg                                                                              <  2:4729734/101‑1 (MQ=255)

                    cTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGt                                                                             <  2:4730058/101‑1 (MQ=255)

                     tGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTg                                                                            >  2:4730185/1‑101 (MQ=255)

                       gCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGAt                                                                          <  1:4729450/101‑1 (MQ=255)

                       gCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGAt                                                                          <  1:4729806/101‑1 (MQ=255)

                       gCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGAt                                                                          <  1:4729741/101‑1 (MQ=255)

                        cGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATc                                                                         <  2:4729629/101‑1 (MQ=255)

                        cGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATc                                                                         >  1:4730187/1‑101 (MQ=255)

                         ggTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCg                                                                        >  2:4730189/1‑101 (MQ=255)

                          gTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGt                                                                       >  2:4730191/1‑101 (MQ=255)

                           ttGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTa                                                                      >  1:4730192/1‑101 (MQ=255)

                           ttGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTa                                                                      <  1:4730050/101‑1 (MQ=255)

                            tGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTag                                                                     <  1:4729820/101‑1 (MQ=255)

                             gAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTaga                                                                    <  2:4729824/101‑1 (MQ=255)

                              aaCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaa                                                                   >  2:4730196/1‑101 (MQ=255)

                               aCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGGTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaag                                                                  <  2:4729870/101‑1 (MQ=255)

                               aCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaag                                                                  >  1:4730197/1‑101 (MQ=255)

                                cTGCATGAAGGCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaaga                                                                 <  2:4729445/101‑1 (MQ=255)

                                cTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaaga                                                                 <  1:4729672/101‑1 (MQ=255)

                                 tGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAt                                                                >  2:4730199/1‑101 (MQ=255)

                                      gAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCgg                                                           <  1:4729544/101‑1 (MQ=255)

                                       aaGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGt                                                          >  2:4730203/1‑101 (MQ=255)

                                         gACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGc                                                        >  1:4730205/1‑101 (MQ=255)

                                          aCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTag                                                                     <  2:4729873/87‑1 (MQ=255)

                                          aCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCa                                                       >  1:4730207/1‑101 (MQ=255)

                                           cGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCAc                                                      >  2:4730209/1‑101 (MQ=255)

                                            gATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACg                                                     >  1:4730210/1‑101 (MQ=255)

                                             aTGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACgg                                                    <  1:4729752/101‑1 (MQ=255)

                                             aTGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACgg                                                    <  2:4729577/101‑1 (MQ=255)

                                             aTGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACgg                                                    >  2:4730213/1‑101 (MQ=255)

                                             aTGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACgg                                                    <  1:4729731/101‑1 (MQ=255)

                                                ttCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGcc                                                 >  1:4730215/1‑101 (MQ=255)

                                                ttCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGcc                                                 <  2:4729746/101‑1 (MQ=255)

                                                ttCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGcc                                                 <  2:4729974/101‑1 (MQ=255)

                                                 tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGc                                                  >  1:4730218/1‑99 (MQ=255)

                                                  ccAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGc                                               >  2:4730219/1‑101 (MQ=255)

                                                    aGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCAc                                             <  2:4729708/101‑1 (MQ=255)

                                                    aGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCAc                                             <  2:4729838/101‑1 (MQ=255)

                                                     gATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTgg                                                                                    >  2:4730223/1‑61 (MQ=255)

                                                      aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACgg                                           <  1:4730124/101‑1 (MQ=255)

                                                      aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACgg                                           <  1:4729848/101‑1 (MQ=255)

                                                      aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACgg                                           >  2:4730225/1‑101 (MQ=255)

                                                       tctcAATGTAGCGGTCGCCGTCTTCTTCCGGCCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGa                                          >  2:4730227/1‑101 (MQ=255)

                                                        ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                         >  1:4730229/1‑101 (MQ=255)

                                                         tcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAcc                                        <  1:4729878/101‑1 (MQ=255)

                                                          cAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCa                                       <  2:4729960/101‑1 (MQ=255)

                                                             tGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgtg                                    <  2:4729928/101‑1 (MQ=255)

                                                              gTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGt                                                          >  2:4730232/1‑78 (MQ=255)

                                                              gTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgtgt                                   <  2:4729902/101‑1 (MQ=255)

                                                                aGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCa                                 >  1:4730233/1‑101 (MQ=255)

                                                                 gCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                                   <  1:4730159/82‑1 (MQ=255)

                                                                  cGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAcc                               >  1:4730235/1‑101 (MQ=255)

                                                                   ggTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGc                                                  >  1:4730237/1‑81 (MQ=255)

                                                                        ccGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTg                         <  2:4729937/101‑1 (MQ=255)

                                                                        ccGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTg                         <  2:4729898/101‑1 (MQ=255)

                                                                          ggcttcttCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTc                                                                                  >  2:4730242/3‑42 (MQ=38)

                                                                          gTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGcc                       >  1:4730241/1‑101 (MQ=255)

                                                                             ttcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGt                                                                             >  1:4730247/1‑44 (MQ=255)

                                                                             ttcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGa                    >  1:4730245/1‑101 (MQ=255)

                                                                              tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGaa                   >  2:4730248/1‑101 (MQ=255)

                                                                                ttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGt                 >  2:4730250/1‑101 (MQ=255)

                                                                                  ccGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTg               <  2:4729980/101‑1 (MQ=255)

                                                                                   cGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGt              >  2:4730252/1‑101 (MQ=255)

                                                                                   cGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGt              <  2:4729623/101‑1 (MQ=255)

                                                                                     ggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAttt                                                              <  1:4729817/51‑1 (MQ=255)

                                                                                     ggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCAc                                             <  1:4729981/68‑1 (MQ=255)

                                                                                     ggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCa            <  1:4729793/101‑1 (MQ=255)

                                                                                     ggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCa            >  2:4730255/1‑101 (MQ=255)

                                                                                      gCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAg           <  1:4729864/101‑1 (MQ=255)

                                                                                       cTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTAAGa          >  2:4730258/1‑101 (MQ=255)

                                                                                        ttCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGAt         >  2:4730260/1‑101 (MQ=255)

                                                                                         tCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACgg                                           <  1:4730179/66‑1 (MQ=255)

                                                                                         tCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATg        >  1:4730261/1‑101 (MQ=255)

                                                                                          cccGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGt                 <  1:4729961/91‑1 (MQ=255)

                                                                                          cccGGAGCCCCCCCCCAAATGTGGGCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGc       <  2:4729777/101‑1 (MQ=255)

                                                                                           ccGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCg      >  2:4730263/1‑101 (MQ=255)

                                                                                           ccGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCg      <  1:4730025/101‑1 (MQ=255)

                                                                                             ggAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTa    <  1:4729774/101‑1 (MQ=255)

                                                                                             ggAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTa    <  2:4729737/101‑1 (MQ=255)

                                                                                             ggAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTa    >  1:4730266/1‑101 (MQ=255)

                                                                                              gAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTAt   <  1:4729930/101‑1 (MQ=255)

                                                                                               aGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCAt                            <  2:4729943/75‑1 (MQ=255)

GGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATG  >  NC_000913/2821326‑2821521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`