Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,053,229 T→C Y97C (TAC→TGC)  ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,053,2290TC99.7% 1405.4 / NA 339Y97C (TAC→TGC) ubiH2‑octaprenyl‑6‑methoxyphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (177/161);  minor base G (1/0);  total (178/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

GGCAATGCAGCGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGTAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGAC  >  NC_000913/3053131‑3053326
                                                                                                  |                                                                                                 
   aaTGCAGCGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAAtct                                                                                              >  2:5141230/1‑101 (MQ=255)
       cAGCGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTg                                                                                          >  1:5141234/1‑101 (MQ=255)
       cAGCGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTg                                                                                          <  1:5140812/101‑1 (MQ=255)
        aGCGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgc                                                                                         <  2:5140732/101‑1 (MQ=255)
        aGCGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgc                                                                                         >  2:5141237/1‑101 (MQ=255)
        aGCGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgc                                                                                         <  1:5140787/101‑1 (MQ=255)
        aGCGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgc                                                                                         >  1:5141239/1‑101 (MQ=255)
         gCGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgcg                                                                                        >  1:5141245/1‑101 (MQ=255)
          cGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgcgg                                                                                       >  2:5141246/1‑101 (MQ=255)
          cGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgcgg                                                                                       <  1:5140926/101‑1 (MQ=255)
            ttACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgcggcg                                                                                     <  2:5140966/101‑1 (MQ=255)
            ttACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgcggcg                                                                                     >  1:5141250/1‑101 (MQ=255)
               cGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAgg                                                                                  >  2:5141254/1‑101 (MQ=255)
                gCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAggg                                                                                 <  1:5140696/101‑1 (MQ=255)
                  cAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTg                                                                               >  2:5141258/1‑101 (MQ=255)
                   aGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGa                                                                              >  2:5141262/1‑101 (MQ=255)
                   aGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGa                                                                              >  1:5141263/1‑101 (MQ=255)
                     gTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACa                                                                            <  2:5140996/101‑1 (MQ=255)
                      tGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACaa                                                                           >  2:5141268/1‑101 (MQ=255)
                      tGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACaa                                                                           <  2:5140892/101‑1 (MQ=255)
                      tGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACaa                                                                           <  2:5140631/101‑1 (MQ=255)
                       gCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACaaa                                                                          >  1:5141270/1‑101 (MQ=255)
                        cTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgcggcg                                                                                     <  2:5140919/89‑1 (MQ=255)
                        cTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAAt                                                                         >  2:5141274/1‑101 (MQ=255)
                         tttACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATc                                                                        >  1:5141276/1‑101 (MQ=255)
                          ttACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATcc                                                                       <  2:5140972/101‑1 (MQ=255)
                           tACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCa                                                                      >  1:5141282/1‑101 (MQ=255)
                           tACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCa                                                                      >  1:5141285/1‑101 (MQ=255)
                            aCGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAg                                                                     >  2:5141287/1‑101 (MQ=255)
                             cGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGc                                                                    <  2:5141055/101‑1 (MQ=255)
                              gcagcaATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCg                                                                   >  1:5141292/1‑101 (MQ=255)
                               cagcaATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGt                                                                  <  2:5140868/101‑1 (MQ=255)
                               cagcaATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGt                                                                  >  1:5141295/1‑101 (MQ=255)
                                agcaATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTgc                                                                                         <  2:5140902/77‑1 (MQ=255)
                                agcaATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTg                                                                 <  2:5140934/101‑1 (MQ=255)
                                agcaATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTg                                                                 >  2:5141299/1‑101 (MQ=255)
                                  caATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGAc                                                               >  1:5141301/1‑101 (MQ=255)
                                  caATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGAc                                                               >  2:5141303/1‑101 (MQ=255)
                                   aaTGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGAcc                                                              <  2:5140513/101‑1 (MQ=255)
                                   aaTGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGAcc                                                              <  2:5141144/101‑1 (MQ=255)
                                    aTGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCa                                                             >  1:5141309/1‑101 (MQ=255)
                                    aTGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCa                                                             <  2:5140835/101‑1 (MQ=255)
                                      gCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACg                                                           >  2:5141312/1‑101 (MQ=255)
                                        aaaCAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGAt                                                         >  2:5141317/1‑101 (MQ=255)
                                        aaaCAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGAt                                                         >  1:5141316/1‑101 (MQ=255)
                                         aaCAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATc                                                        <  1:5141020/101‑1 (MQ=255)
                                          aCAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACaaa                                                                          <  1:5141104/82‑1 (MQ=255)
                                          aCAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCg                                                       >  2:5141321/1‑101 (MQ=255)
                                             gCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTg                                                    <  1:5140988/101‑1 (MQ=255)
                                              ccGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGa                                                   <  1:5140841/101‑1 (MQ=255)
                                              ccGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGa                                                   <  1:5140544/101‑1 (MQ=255)
                                               cGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGAc                                                  <  1:5140946/101‑1 (MQ=255)
                                               cGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGAc                                                  >  1:5141327/1‑101 (MQ=255)
                                                gTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACa                                                 >  2:5141328/1‑101 (MQ=255)
                                                 ttGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACAt                                                <  2:5140885/101‑1 (MQ=255)
                                                  tGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATg                                               <  2:5140945/101‑1 (MQ=255)
                                                    cccGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCa                                             >  2:5141334/1‑101 (MQ=255)
                                                    cccGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCa                                             >  1:5141337/1‑101 (MQ=255)
                                                     ccGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATc                                                        <  1:5140675/89‑1 (MQ=255)
                                                     ccGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCAc                                            <  2:5141015/101‑1 (MQ=255)
                                                     ccGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCAc                                            <  1:5141038/101‑1 (MQ=255)
                                                      cGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACg                                           >  2:5141341/1‑101 (MQ=255)
                                                       gACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACgg                                          >  1:5141343/1‑101 (MQ=255)
                                                       gACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACgg                                          <  2:5140697/101‑1 (MQ=255)
                                                          aTTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACggtgg                                       <  2:5140823/101‑1 (MQ=255)
                                                           ttGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACggtggt                                      >  1:5141349/1‑101 (MQ=255)
                                                           ttGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACggtgg                                       >  2:5141350/1‑100 (MQ=255)
                                                            tgtgCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGgtggtg                                     >  1:5141353/1‑101 (MQ=255)
                                                            tgtgCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGgtggtg                                     <  1:5140627/101‑1 (MQ=255)
                                                               gCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATg                                  <  2:5141031/101‑1 (MQ=255)
                                                                 aaTTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGGCCACGAt                                                         <  1:5140509/76‑1 (MQ=255)
                                                                 aaTTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGc                                >  2:5141360/1‑101 (MQ=255)
                                                                 aaTTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGc                                >  1:5141359/1‑101 (MQ=255)
                                                                  aTTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCa                               <  1:5140752/101‑1 (MQ=255)
                                                                  aTTCGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCa                               <  2:5140651/101‑1 (MQ=255)
                                                                     cGACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGtt                            <  2:5140850/101‑1 (MQ=255)
                                                                      gACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTg                           >  2:5141365/1‑101 (MQ=255)
                                                                      gACAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTg                           <  1:5140833/101‑1 (MQ=255)
                                                                        cAACCTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACAt                                                <  1:5140861/78‑1 (MQ=255)
                                                                           ccTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAg                              >  2:5141371/1‑93 (MQ=255)
                                                                           ccTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCaa                      >  1:5141369/1‑101 (MQ=255)
                                                                           ccTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCaa                      <  2:5140804/101‑1 (MQ=255)
                                                                            cTGTCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAAt                     >  1:5141374/1‑101 (MQ=255)
                                                                               tCCCAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCg                  <  2:5140761/101‑1 (MQ=255)
                                                                                 ccAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGcc                >  1:5141378/1‑101 (MQ=255)
                                                                                  cAGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCa               <  1:5140898/101‑1 (MQ=255)
                                                                                   aGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCag              >  1:5141381/1‑101 (MQ=255)
                                                                                   aGCGCCGCCAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCag              <  2:5140664/101‑1 (MQ=255)
                                                                                    gcgcCGCCAGTGGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACg                                           <  1:5140865/71‑1 (MQ=255)
                                                                                      gccgccAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAgaga           >  1:5141387/1‑101 (MQ=255)
                                                                                      gccgccAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAgaga           >  2:5141386/1‑101 (MQ=255)
                                                                                        cgccAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGAtt         <  2:5140881/101‑1 (MQ=255)
                                                                                          ccAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGc                                >  1:5141394/1‑76 (MQ=255)
                                                                                          ccAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGc       >  2:5141393/1‑101 (MQ=255)
                                                                                          ccAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGc       <  1:5141052/101‑1 (MQ=255)
                                                                                           cAGTTGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGcc      >  1:5141397/1‑101 (MQ=255)
                                                                                              ttGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTg                                                                 <  1:5140911/39‑1 (MQ=255)
                                                                                              ttGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGa   >  1:5141404/1‑101 (MQ=255)
                                                                                               tGGCAATCTTCTGCGTCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGAc  >  1:5141407/1‑101 (MQ=255)
                                                                                               tGGCAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGAc  <  1:5140773/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GGCAATGCAGCGTTACGCCAGGTGCTTTACGCAGCAATGCAAACAGCCGTTGCCCGACATTGTGCAATTCGACAACCTGTCCCAGCGCCGCCAGTTGGTAATCTTCTGCGGCGAGGGTGACAAATCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGAC  >  NC_000913/3053131‑3053326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑