BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	82,046	A→G	V495A (GTT→GCT)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	82,046	0	A	G	100.0%	1403.9 / NA	331	V495A (GTT→GCT)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (177/154); total (177/154)

Only 100 of 337 total aligned reads displayed.

TTCTTCGACATCACACGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTG  >  NC_000913/81948‑82143

ttcttcGACATCACACGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCg                                                                                                 <  1:169555/101‑1 (MQ=255)

ttcttcGACATCACACGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCg                                                                                                 >  1:170288/1‑101 (MQ=255)

  cttcGACATCACACGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTg                                                                                               <  1:169815/101‑1 (MQ=255)

    tcGACATCACACGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCa                                                                                             <  2:169784/101‑1 (MQ=255)

     cGACATCACACGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCAc                                                                                            >  1:170294/1‑101 (MQ=255)

       aCATCACACGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCa                                                                                          <  2:169888/101‑1 (MQ=255)

        cATCACACGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCAt                                                                                         >  1:170300/1‑101 (MQ=255)

         aTCACACGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATg                                                                                        >  2:170302/1‑101 (MQ=255)

           cacaCGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGc                                                                                      >  1:170305/1‑101 (MQ=255)

             cacGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCtt                                                                                    <  2:169772/101‑1 (MQ=255)

              acGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCttt                                                                                   >  1:170309/1‑101 (MQ=255)

              acGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCttt                                                                                   >  2:170310/1‑101 (MQ=255)

              acGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCttt                                                                                   <  1:169915/101‑1 (MQ=255)

              acGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCttt                                                                                   <  1:169901/101‑1 (MQ=255)

               cGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTg                                                                                  <  2:169585/101‑1 (MQ=255)

                 gTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGc                                                                                <  2:169742/101‑1 (MQ=255)

                  tttCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCa                                                                               >  1:170315/1‑101 (MQ=255)

                  tttCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCa                                                                               >  2:170317/1‑101 (MQ=255)

                   ttCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAg                                                                              <  2:169837/101‑1 (MQ=255)

                       ttgttgTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGa                                                                          >  1:170323/1‑101 (MQ=255)

                       ttgttgTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGa                                                                          >  2:170324/1‑101 (MQ=255)

                        tgttgtTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGAc                                                                         >  2:170329/1‑101 (MQ=255)

                         gttgttTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACt                                                                        >  1:170330/1‑101 (MQ=255)

                             ttttCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGAc                                                                    <  2:170059/101‑1 (MQ=255)

                              tttCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACa                                                                   >  2:170336/1‑101 (MQ=255)

                               ttCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACaa                                                                  >  1:170338/1‑101 (MQ=255)

                                tCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAat                                                                 <  2:170025/101‑1 (MQ=255)

                                tCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAat                                                                 <  2:170039/101‑1 (MQ=255)

                                 cGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAata                                                                >  2:170343/1‑101 (MQ=255)

                                 cGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAata                                                                <  2:169812/101‑1 (MQ=255)

                                 cGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAata                                                                <  1:169729/101‑1 (MQ=255)

                                  gTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAatat                                                               >  1:170345/1‑101 (MQ=255)

                                   ttGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATc                                                              <  1:169992/101‑1 (MQ=255)

                                   ttGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATc                                                              <  1:170042/101‑1 (MQ=255)

                                    tgtgTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCg                                                             >  2:170349/1‑101 (MQ=255)

                                    tgtgTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCg                                                             >  1:170350/1‑101 (MQ=255)

                                     gtgtTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCgg                                                            <  2:170006/101‑1 (MQ=255)

                                      tgtTGAGCATTGCGTTGCAACTCTTTATCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGt                                                           >  1:170355/1‑101 (MQ=255)

                                       gtTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTa                                                          <  2:170083/101‑1 (MQ=255)

                                       gtTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTa                                                          <  2:169683/101‑1 (MQ=255)

                                       gtTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTa                                                          <  1:169767/101‑1 (MQ=255)

                                         tGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGc                                                        <  2:169974/101‑1 (MQ=255)

                                         tGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGc                                                        >  2:170362/1‑101 (MQ=255)

                                         tGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGc                                                        >  1:170360/1‑101 (MQ=255)

                                           aGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCa                                                      <  1:169738/101‑1 (MQ=255)

                                            gCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAg                                                     >  1:170365/1‑101 (MQ=255)

                                              tttGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGc                                                   <  2:169997/101‑1 (MQ=255)

                                               ttGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGcc                                                  <  2:169614/101‑1 (MQ=255)

                                               ttGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGcc                                                  >  1:170371/1‑101 (MQ=255)

                                               ttGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGcc                                                  <  2:170015/101‑1 (MQ=255)

                                                tGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCg                                                 >  2:170373/1‑101 (MQ=255)

                                                 gCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGa                                                >  1:170378/1‑101 (MQ=255)

                                                 gCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGa                                                >  2:170377/1‑101 (MQ=255)

                                                 gCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGa                                                <  1:169619/101‑1 (MQ=255)

                                                     tGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGcc                                            >  2:170385/1‑101 (MQ=255)

                                                       cAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGt                                          >  1:170386/1‑101 (MQ=255)

                                                         aCTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTgg                                        >  2:170390/1‑101 (MQ=255)

                                                          ctctTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGa                                       >  1:170392/1‑101 (MQ=255)

                                                           tctTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCg                                           >  1:170397/1‑96 (MQ=255)

                                                            ctTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAg                                     <  1:169864/101‑1 (MQ=255)

                                                             tttttCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAgc                                    >  2:170401/1‑101 (MQ=255)

                                                             tttttCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAgc                                    <  2:170169/101‑1 (MQ=255)

                                                              ttttCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAgcg                                   <  2:169725/101‑1 (MQ=255)

                                                              ttttCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAgcg                                   >  1:170403/1‑101 (MQ=255)

                                                               tttCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAgcgg                                  >  1:170406/1‑101 (MQ=255)

                                                                ttCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAgcggc                                 <  2:169936/101‑1 (MQ=255)

                                                                ttCGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAgcggc                                 <  1:169937/101‑1 (MQ=255)

                                                                  cGACTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGa                               >  1:170411/1‑101 (MQ=255)

                                                                    aCTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGAcc                             >  2:170415/1‑101 (MQ=255)

                                                                    aCTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGAcc                             <  1:170012/101‑1 (MQ=255)

                                                                     cTTCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCg                            >  1:170416/1‑101 (MQ=255)

                                                                      ttCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGt                           >  2:170421/1‑101 (MQ=255)

                                                                       tCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGtt                          >  1:170422/1‑101 (MQ=255)

                                                                       tCTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACAGtt                          <  1:169843/101‑1 (MQ=255)

                                                                        cTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTg                         >  2:170426/1‑101 (MQ=255)

                                                                        cTGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTg                         <  2:169879/101‑1 (MQ=255)

                                                                         tGCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAg                                     >  2:170430/1‑88 (MQ=255)

                                                                          gCGGCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCTTTGTa                       <  2:169955/101‑1 (MQ=255)

                                                                             gCACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGtt                    >  2:170434/1‑101 (MQ=255)

                                                                              cACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTg                                         <  2:169804/79‑1 (MQ=255)

                                                                              cACGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTa                   <  2:169863/101‑1 (MQ=255)

                                                                               aCGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAg                  >  1:170438/1‑101 (MQ=255)

                                                                                cGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGc                 >  2:170442/1‑101 (MQ=255)

                                                                                cGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGc                 <  2:169917/101‑1 (MQ=255)

                                                                                cGCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGc                 <  1:169360/101‑1 (MQ=255)

                                                                                 gCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGcg                >  1:170443/1‑101 (MQ=255)

                                                                                 gCCAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGcg                <  2:169984/101‑1 (MQ=255)

                                                                                   cAGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGcgac              >  1:170448/1‑101 (MQ=255)

                                                                                    aGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGcgacg             >  2:170449/1‑101 (MQ=255)

                                                                                    aGATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGcgacg             <  2:169548/101‑1 (MQ=255)

                                                                                     gATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGcgacga            >  1:170456/1‑101 (MQ=255)

                                                                                     gATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGcgacga            >  2:170454/1‑101 (MQ=255)

                                                                                     gATATTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGcgacga            <  2:170192/101‑1 (MQ=255)

                                                                                      atatTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGat           <  2:169900/101‑1 (MQ=255)

                                                                                       tatTGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGata          >  2:170460/1‑101 (MQ=255)

                                                                                         ttGTTCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATc        <  1:170100/101‑1 (MQ=255)

                                                                                            ttCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCAc     <  1:169970/101‑1 (MQ=255)

                                                                                             tCAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCAcc    >  2:170465/1‑101 (MQ=255)

                                                                                              cAGAGCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCt   <  2:170278/101‑1 (MQ=255)

                                                                                               agagCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTg  >  1:170469/1‑101 (MQ=255)

TTCTTCGACATCACACGGTTTCCTTGTTGTTTTCGTTGTGTTGAGCTTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTG  >  NC_000913/81948‑82143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`