BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,320,256	C→T	G377R (GGG→AGG)	trpD ←	anthranilate synthase subunit TrpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,320,256	0	C	T	100.0%	1504.3 / NA	351	G377R (GGG→AGG)	trpD	anthranilate synthase subunit TrpD
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (179/172); total (179/172)

Only 100 of 351 total aligned reads displayed.

CCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACA  >  NC_000913/1320158‑1320353

ccccAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCa                                                                                                 >  2:2496683/1‑101 (MQ=255)

ccccAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCa                                                                                                 <  2:2496292/101‑1 (MQ=255)

  ccAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGc                                                                                               >  1:2496686/1‑101 (MQ=255)

  ccAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGc                                                                                               <  1:2496460/101‑1 (MQ=255)

   cAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGca                                                                                              >  2:2496688/1‑101 (MQ=255)

    aGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGcac                                                                                             <  2:2496259/101‑1 (MQ=255)

      cACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACAt                                                                                           >  1:2496693/1‑101 (MQ=255)

      cACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACAt                                                                                           <  1:2496220/101‑1 (MQ=255)

           gcAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAAc                                                                                      >  1:2496697/1‑101 (MQ=255)

            cAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACa                                                                                     >  2:2496701/1‑101 (MQ=255)

            cAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACa                                                                                     <  2:2496446/101‑1 (MQ=255)

              aGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAgg                                                                                   >  1:2496705/1‑101 (MQ=255)

                  ttCGGCAATCGTCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGc                                                                               <  1:2496150/101‑1 (MQ=255)

                  ttCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGc                                                                               >  1:2496709/1‑101 (MQ=255)

                    cGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCgg                                                                             <  1:2496316/101‑1 (MQ=255)

                      gCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGt                                                                           <  1:2496305/101‑1 (MQ=255)

                      gCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGt                                                                           <  1:2496105/101‑1 (MQ=255)

                        aaTCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAggg                                                                                  >  1:2496722/1‑92 (MQ=255)

                        aaTCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGttt                                                                         >  2:2496721/1‑101 (MQ=255)

                        aaTCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGttt                                                                         >  1:2496718/1‑101 (MQ=255)

                           cGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAAAGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCa                                                                      <  2:2495958/101‑1 (MQ=255)

                            ggCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAg                                                                     >  1:2496727/1‑101 (MQ=255)

                            ggCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAg                                                                     <  2:2496280/101‑1 (MQ=255)

                             gcagcaCCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGt                                                                    <  2:2496326/101‑1 (MQ=255)

                              cagcaCCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGtt                                                                   <  2:2496229/101‑1 (MQ=255)

                              cagcaCCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGtt                                                                   <  1:2496193/101‑1 (MQ=255)

                               agcaCCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTg                                                                  <  1:2496129/101‑1 (MQ=255)

                               agcaCCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTg                                                                  >  2:2496731/1‑101 (MQ=255)

                               agcaCCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTg                                                                  <  2:2496183/101‑1 (MQ=255)

                                gcaCCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGc                                                                 <  2:2496289/101‑1 (MQ=255)

                                gcaCCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGc                                                                 <  2:2496338/101‑1 (MQ=255)

                                  aCCAGTTCCGGACTATAAACGCCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTg                                                                                         <  2:2496064/75‑1 (MQ=255)

                                  aCCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTg                                                               >  1:2496735/1‑101 (MQ=255)

                                  aCCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTg                                                               <  1:2495990/101‑1 (MQ=255)

                                    cAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGc                                                             <  1:2496362/101‑1 (MQ=255)

                                     aGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCg                                                            >  1:2496740/1‑101 (MQ=255)

                                     aGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCg                                                            >  2:2496741/1‑101 (MQ=255)

                                       ttCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCg                                                            >  1:2496745/1‑99 (MQ=255)

                                         ccGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAAcc                                                        >  2:2496750/1‑101 (MQ=255)

                                           ggACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCgg                                                      >  1:2496754/1‑101 (MQ=255)

                                            gACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGc                                                     <  1:2496073/101‑1 (MQ=255)

                                            gACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGc                                                     <  2:2496474/101‑1 (MQ=255)

                                             aCTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCa                                                    >  1:2496758/1‑101 (MQ=255)

                                              cTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCAt                                                   <  1:2496356/101‑1 (MQ=255)

                                               tataAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATc                                                  <  1:2496179/101‑1 (MQ=255)

                                                 taAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATcgc                                                <  1:2496331/101‑1 (MQ=255)

                                                 taAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATcgc                                                >  2:2496762/1‑101 (MQ=255)

                                                   aaCACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGt                                              <  1:2496600/101‑1 (MQ=255)

                                                    acacCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTg                                             >  1:2496767/1‑101 (MQ=255)

                                                    acacCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTg                                             >  2:2496769/1‑101 (MQ=255)

                                                     cacCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTgg                                            <  1:2496158/101‑1 (MQ=255)

                                                      acCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGc                                           <  1:2496271/101‑1 (MQ=255)

                                                       ccAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCg                                          <  2:2496348/101‑1 (MQ=255)

                                                       ccAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCg                                          >  1:2496773/1‑101 (MQ=255)

                                                        cAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCgg                                         <  2:2496152/101‑1 (MQ=255)

                                                        cAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGGGc                                                                               >  2:2496776/1‑63 (MQ=255)

                                                         aaTTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGa                                        <  1:2496486/101‑1 (MQ=255)

                                                          aTTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGaa                                       <  2:2496503/101‑1 (MQ=255)

                                                           ttAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAAt                                      >  1:2496780/1‑101 (MQ=255)

                                                            tAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATc                                     <  2:2496320/101‑1 (MQ=255)

                                                            tAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATc                                     >  1:2496783/1‑101 (MQ=255)

                                                             aaCGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATcc                                    >  2:2496787/1‑101 (MQ=255)

                                                             aaCGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATcc                                    <  1:2496261/101‑1 (MQ=255)

                                                             aaCGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATcc                                    <  2:2496276/101‑1 (MQ=255)

                                                              aCGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCg                                   >  1:2496791/1‑101 (MQ=255)

                                                               cGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAggg                                                                                  >  2:2496793/1‑53 (MQ=255)

                                                                gCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCgg                                  >  2:2496796/1‑100 (MQ=255)

                                                                  cAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGc                                                                               >  1:2496805/1‑53 (MQ=255)

                                                                  cAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCgg                                  >  2:2496802/1‑98 (MQ=255)

                                                                  cAGCGGCGGATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGgtgt                               <  1:2496346/101‑1 (MQ=255)

                                                                      ggcggATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATa                           >  2:2496812/1‑101 (MQ=255)

                                                                      ggcggATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATa                           <  2:2496364/101‑1 (MQ=255)

                                                                      ggcggATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATa                           >  1:2496809/1‑101 (MQ=255)

                                                                       gcggATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATAc                          <  1:2496528/101‑1 (MQ=255)

                                                                       gcggATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATAc                          <  1:2496246/101‑1 (MQ=255)

                                                                       gcggATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATAc                          >  1:2496813/1‑101 (MQ=255)

                                                                         ggATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACtt                        <  2:2496382/101‑1 (MQ=255)

                                                                         ggATGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACtt                        >  1:2496818/1‑101 (MQ=255)

                                                                           aTGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCg                      >  1:2496821/1‑101 (MQ=255)

                                                                           aTGCGCCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCg                      <  2:2496518/101‑1 (MQ=255)

                                                                             gcgcCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGgc                    >  2:2496827/1‑101 (MQ=255)

                                                                             gcgcCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGgc                    >  1:2496828/1‑101 (MQ=255)

                                                                             gcgcCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGgc                    <  1:2496412/101‑1 (MQ=255)

                                                                              cgcCGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGgcg                   <  2:2496709/101‑1 (MQ=255)

                                                                                ccGGGTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCa                 >  1:2496832/1‑101 (MQ=255)

                                                                                 cGGGTTAATCAATGGCCTCAGCACATTGAACAGGGAGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCaa                >  2:2496836/1‑101 (MQ=255)

                                                                                  gggTTAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCaaa               >  1:2496839/1‑101 (MQ=255)

                                                                                     ttAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAagag            >  2:2496845/1‑101 (MQ=255)

                                                                                      tAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAgg           <  2:2496409/101‑1 (MQ=255)

                                                                                      tAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAgg           >  2:2496848/1‑101 (MQ=255)

                                                                                      tAATCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAgg           >  1:2496849/1‑101 (MQ=255)

                                                                                       aaTCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGa          <  1:2496438/101‑1 (MQ=255)

                                                                                        aTCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGaa         <  1:2496445/101‑1 (MQ=255)

                                                                                        aTCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGaa         <  1:2496374/101‑1 (MQ=255)

                                                                                         tCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGaaa        >  1:2496856/1‑101 (MQ=255)

                                                                                         tCAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGaaa        >  2:2496855/1‑101 (MQ=255)

                                                                                          cAATGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAAc       >  1:2496861/1‑101 (MQ=255)

                                                                                           aaTGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACa      <  2:2496398/101‑1 (MQ=255)

                                                                                            aTGGCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACAt     <  1:2496383/101‑1 (MQ=255)

                                                                                               gCCTCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATaca  >  1:2496866/1‑101 (MQ=255)

CCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACA  >  NC_000913/1320158‑1320353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`