BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,115,377	T→C	N174D (AAC→GAC)	wcaM ←	putative colanic acid biosynthesis protein WcaM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,115,377	0	T	C	100.0%	1525.5 / NA	360	N174D (AAC→GAC)	wcaM	putative colanic acid biosynthesis protein WcaM
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (155/205); total (155/205)

Only 100 of 360 total aligned reads displayed.

CAAAATATCACGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTTATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATA  >  NC_000913/2115279‑2115474

cAAAATATCACGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCAt                                                                                                 <  2:3856742/101‑1 (MQ=255)

  aaaTATCACGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGa                                                                                               >  2:3857529/1‑101 (MQ=255)

  aaaTATCACGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGa                                                                                               <  1:3857230/101‑1 (MQ=255)

  aaaTATCACGGTCGTTAATTGCAACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGa                                                                                               >  1:3857528/1‑101 (MQ=255)

   aaTATCACGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGaa                                                                                              <  1:3857036/101‑1 (MQ=255)

    atatCACGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGaaa                                                                                             <  2:3856813/101‑1 (MQ=255)

     tatCACGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAAt                                                                                            >  2:3857533/1‑101 (MQ=255)

        cACGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATccc                                                                                         >  2:3857540/1‑101 (MQ=255)

        cACGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATccc                                                                                         >  1:3857539/1‑101 (MQ=255)

         aCGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCt                                                                                        <  1:3856601/101‑1 (MQ=255)

         aCGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCt                                                                                        <  1:3857355/101‑1 (MQ=255)

           ggTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAAt                                                                                            >  2:3857548/1‑95 (MQ=255)

           ggTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTgg                                                                                      <  1:3856824/101‑1 (MQ=255)

           ggTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTgg                                                                                      >  1:3857547/1‑101 (MQ=255)

            gTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGc                                                                                     <  2:3857185/101‑1 (MQ=255)

               gTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTa                                                                                  <  1:3857082/101‑1 (MQ=255)

               gTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTa                                                                                  >  1:3857554/1‑101 (MQ=255)

               gTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTa                                                                                  >  2:3857556/1‑101 (MQ=255)

                 tAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAg                                                                                <  1:3856808/101‑1 (MQ=255)

                  aaTTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTg                                                                                       >  1:3857562/1‑93 (MQ=255)

                    ttGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATg                                                                             >  2:3857565/1‑101 (MQ=255)

                     tGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATgg                                                                            >  1:3857567/1‑101 (MQ=255)

                          cGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTaa                                                                       >  2:3857573/1‑101 (MQ=255)

                             tCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTa                                                                    >  1:3857578/1‑101 (MQ=255)

                             tCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTa                                                                    <  1:3856925/101‑1 (MQ=255)

                             tCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTa                                                                    >  2:3857577/1‑101 (MQ=255)

                              ccATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAg                                                                   <  2:3856967/101‑1 (MQ=255)

                                aTTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCg                                                                 <  2:3856892/101‑1 (MQ=255)

                                aTTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCg                                                                 <  1:3857011/101‑1 (MQ=255)

                                 ttCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGt                                                                <  2:3857061/101‑1 (MQ=255)

                                 ttCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGt                                                                <  1:3856932/101‑1 (MQ=255)

                                  tCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTg                                                               <  2:3857264/101‑1 (MQ=255)

                                  tCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTg                                                               <  1:3856868/101‑1 (MQ=255)

                                     aTGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCt                                                            <  2:3857469/101‑1 (MQ=255)

                                      tGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTa                                                           <  1:3857247/101‑1 (MQ=255)

                                      tGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTa                                                           <  1:3857256/101‑1 (MQ=255)

                                         cGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACg                                                        <  1:3857050/101‑1 (MQ=255)

                                          gTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGc                                                                  >  1:3857586/1‑90 (MQ=255)

                                          gTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACgg                                                       >  2:3857585/1‑101 (MQ=255)

                                            cGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTc                                                     >  1:3857590/1‑101 (MQ=255)

                                             gCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCa                                                    <  1:3857143/101‑1 (MQ=255)

                                              ccTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAg                                                   <  1:3857065/101‑1 (MQ=255)

                                              ccTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAg                                                   <  1:3857298/101‑1 (MQ=255)

                                               cTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAgg                                                  >  2:3857595/1‑101 (MQ=255)

                                                 tGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGtt                                                >  2:3857601/1‑101 (MQ=255)

                                                 tGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGtt                                                >  1:3857600/1‑101 (MQ=255)

                                                     aGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCg                                            <  2:3856923/101‑1 (MQ=255)

                                                     aGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCg                                            <  2:3857170/101‑1 (MQ=255)

                                                     aGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCg                                            >  1:3857607/1‑101 (MQ=255)

                                                      gTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCga                                           <  2:3856982/101‑1 (MQ=255)

                                                         gCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCgatga                                        >  2:3857612/1‑101 (MQ=255)

                                                         gCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCgatga                                        >  1:3857611/1‑101 (MQ=255)

                                                           tGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATc                                      >  2:3857616/1‑101 (MQ=255)

                                                           tGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATc                                      <  1:3857163/101‑1 (MQ=255)

                                                            gAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCa                                     <  2:3857005/101‑1 (MQ=255)

                                                            gAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCa                                     <  2:3857028/101‑1 (MQ=255)

                                                            gAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCa                                     <  1:3857227/101‑1 (MQ=255)

                                                             aaCTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAg                                    <  2:3857202/101‑1 (MQ=255)

                                                              aCTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAgg                                   >  1:3857623/1‑101 (MQ=255)

                                                              aCTTACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAgg                                   >  2:3857622/1‑101 (MQ=255)

                                                                ttACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGtt                                 >  1:3857626/1‑101 (MQ=255)

                                                                 tACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTg                                >  1:3857632/1‑101 (MQ=255)

                                                                 tACAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTg                                <  1:3857073/101‑1 (MQ=255)

                                                                  aCAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTgc                               >  1:3857637/1‑101 (MQ=255)

                                                                  aCAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTgc                               <  2:3856995/101‑1 (MQ=255)

                                                                  aCAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTgc                               <  2:3857383/101‑1 (MQ=255)

                                                                   cAATTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTgcg                              >  2:3857638/1‑101 (MQ=255)

                                                                    aaTTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTgcgc                             >  1:3857642/1‑101 (MQ=255)

                                                                     aTTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCa                            <  2:3857090/101‑1 (MQ=255)

                                                                     aTTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCa                            <  2:3857105/101‑1 (MQ=255)

                                                                     aTTGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCa                            <  2:3857125/101‑1 (MQ=255)

                                                                       tGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATg                          >  2:3857646/1‑101 (MQ=255)

                                                                       tGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATg                          <  2:3857158/101‑1 (MQ=255)

                                                                       tGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATg                          <  1:3857098/101‑1 (MQ=255)

                                                                       tGGTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATg                          <  1:3857427/101‑1 (MQ=255)

                                                                         gTGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGAc                        <  2:3856864/101‑1 (MQ=255)

                                                                          tGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGAcc                       <  1:3856766/101‑1 (MQ=255)

                                                                          tGATGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGAcc                       >  2:3857650/1‑101 (MQ=255)

                                                                            aTGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCg                     <  1:3857331/101‑1 (MQ=255)

                                                                            aTGTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCg                     >  1:3857652/1‑101 (MQ=255)

                                                                              gTTGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCgtt                   >  1:3857656/1‑101 (MQ=255)

                                                                                tGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCgtttg                 <  2:3857345/101‑1 (MQ=255)

                                                                                tGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCgtttg                 >  2:3857658/1‑101 (MQ=255)

                                                                                tGGCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCgtttg                 <  2:3856705/101‑1 (MQ=255)

                                                                                 ggCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCgtttgt                <  1:3857106/101‑1 (MQ=255)

                                                                                 ggCACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCgtttgt                <  1:3857042/101‑1 (MQ=255)

                                                                                   cACCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCgtttgttt              >  1:3857663/1‑101 (MQ=255)

                                                                                    aCCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTa                                               <  2:3857045/67‑1 (MQ=255)

                                                                                    aCCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGtttt             <  2:3857155/101‑1 (MQ=255)

                                                                                    aCCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGtttt             <  1:3857267/101‑1 (MQ=255)

                                                                                    aCCGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGtttt             <  1:3857206/101‑1 (MQ=255)

                                                                                     ccGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGttttt            <  2:3857066/101‑1 (MQ=255)

                                                                                      cGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGttttt            <  1:3856993/100‑1 (MQ=255)

                                                                                      cGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTg           <  2:3856954/101‑1 (MQ=255)

                                                                                      cGATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTg           <  1:3857310/101‑1 (MQ=255)

                                                                                       gATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTgc          >  2:3857669/1‑101 (MQ=255)

                                                                                       gATAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTgc          >  1:3857670/1‑101 (MQ=255)

                                                                                        aTAATCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGtt                                 >  1:3857673/1‑77 (MQ=255)

                                                                                           aTCTGGTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTgccgcc      >  1:3857678/1‑101 (MQ=255)

                                                                                               ggTCATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGata  >  1:3857683/1‑101 (MQ=255)

CAAAATATCACGGTCGTTAATTGCCACGTTCCATTCAATGGCGTCGCCTTGTAAGTCGCTGAACTTACAATTGGTGATGTTGGCACCGATAATCTGGTTATGAAATCCCTGGCGTAAGATGGCGTAATTAGCGTGGCTAACGGTCAGGTTATCGATGATCAGGTTGCGCATGACCCGTTTGTTTTTGCCGCCGATA  >  NC_000913/2115279‑2115474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`