BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,500,163	A→G	H498R (CAC→CGC)	ypdA →	sensory histidine kinase YpdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,500,163	0	A	G	100.0%	1220.9 / NA	287	H498R (CAC→CGC)	ypdA	sensory histidine kinase YpdA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (130/157); total (130/157)

Only 100 of 292 total aligned reads displayed.

TGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTATTG  >  NC_000913/2500065‑2500260

   ccACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCa                                                                                              <  1:4538630/101‑1 (MQ=255)

    cACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCAt                                                                                             >  1:4539259/1‑101 (MQ=255)

    cACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCAt                                                                                             >  2:4539257/1‑101 (MQ=255)

      cGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCg                                                                                           <  1:4538658/101‑1 (MQ=255)

      cGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCg                                                                                           >  2:4539262/1‑101 (MQ=255)

       ggTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGa                                                                                          <  1:4538433/101‑1 (MQ=255)

       ggTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGa                                                                                          <  2:4538725/101‑1 (MQ=255)

        gTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCg                                                                                           <  1:4538587/99‑1 (MQ=255)

        gTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGAt                                                                                         <  2:4538756/101‑1 (MQ=255)

        gTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGAt                                                                                         <  1:4538844/101‑1 (MQ=255)

         tATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATc                                                                                        >  1:4539267/1‑101 (MQ=255)

         tATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATc                                                                                        >  2:4539268/1‑101 (MQ=255)

          aTTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATcc                                                                                       <  2:4538768/101‑1 (MQ=255)

           ttCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCa                                                                                      >  2:4539272/1‑101 (MQ=255)

           ttCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCa                                                                                      <  1:4538706/101‑1 (MQ=255)

              aGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAg                                                                                   <  1:4538849/101‑1 (MQ=255)

               gCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAgg                                                                                  <  1:4538963/101‑1 (MQ=255)

                ccTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGt                                                                                 >  2:4539279/1‑101 (MQ=255)

                 cTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTg                                                                                <  2:4538497/101‑1 (MQ=255)

                  ttGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGa                                                                               >  2:4539282/1‑101 (MQ=255)

                   tGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGAt                                                                              >  1:4539285/1‑101 (MQ=255)

                     cAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTg                                                                            >  2:4539287/1‑101 (MQ=255)

                     cAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTg                                                                            <  2:4538710/101‑1 (MQ=255)

                      aaaGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGa                                                                           >  2:4539292/1‑101 (MQ=255)

                      aaaGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGa                                                                           >  1:4539290/1‑101 (MQ=255)

                         ggTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCg                                                                        <  2:4538550/101‑1 (MQ=255)

                          gTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCgg                                                                       >  2:4539298/1‑101 (MQ=255)

                           tAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCggg                                                                      >  2:4539301/1‑101 (MQ=255)

                             aaGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTc                                                                    >  1:4539306/1‑101 (MQ=255)

                             aaGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTc                                                                    <  2:4538911/101‑1 (MQ=255)

                              aGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCg                                                                   >  2:4539307/1‑101 (MQ=255)

                                 cGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAg                                                                >  2:4539311/1‑101 (MQ=255)

                                   ttGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCg                                                              >  1:4539314/1‑101 (MQ=255)

                                    tGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGa                                                             <  1:4538832/101‑1 (MQ=255)

                                    tGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGGACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGa                                                             >  1:4539316/1‑101 (MQ=255)

                                     gTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGaa                                                            >  2:4539318/1‑101 (MQ=255)

                                      tCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAAt                                                           <  2:4538806/101‑1 (MQ=255)

                                      tCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAAt                                                           <  1:4538901/101‑1 (MQ=255)

                                        aCCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGa                                                         <  1:4538924/101‑1 (MQ=255)

                                        aCCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGa                                                         <  1:4538907/101‑1 (MQ=255)

                                         ccATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGaa                                                        <  2:4539014/101‑1 (MQ=255)

                                         ccATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGaa                                                        >  2:4539322/1‑101 (MQ=255)

                                          cATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGaaa                                                       <  1:4538783/101‑1 (MQ=255)

                                          cATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGaaa                                                       <  2:4538858/101‑1 (MQ=255)

                                           aTCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAAt                                                      <  2:4538940/101‑1 (MQ=255)

                                           aTCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAAt                                                      <  2:4538950/101‑1 (MQ=255)

                                            tCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATg                                                     >  2:4539325/1‑101 (MQ=255)

                                             cAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGc                                                    <  1:4538812/101‑1 (MQ=255)

                                             cAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGc                                                    <  1:4538694/101‑1 (MQ=255)

                                              aGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGcc                                                   >  1:4539328/1‑101 (MQ=255)

                                              aGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGcc                                                   <  1:4538769/101‑1 (MQ=255)

                                               gCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCg                                                  >  2:4539331/1‑101 (MQ=255)

                                                cGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCgg                                                 <  2:4538686/101‑1 (MQ=255)

                                                cGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCgg                                                 >  2:4539334/1‑101 (MQ=255)

                                                  ttGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGc                                               <  1:4539037/101‑1 (MQ=255)

                                                  ttGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGc                                               >  2:4539338/1‑101 (MQ=255)

                                                   tGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCa                                              >  1:4539339/1‑101 (MQ=255)

                                                    gCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCaa                                             <  2:4539048/101‑1 (MQ=255)

                                                    gCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCaa                                             <  1:4538995/101‑1 (MQ=255)

                                                     cAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAAt                                            >  1:4539344/1‑101 (MQ=255)

                                                      agagTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATa                                           >  1:4539347/1‑101 (MQ=255)

                                                        agTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCggg                                                                      >  2:4539349/1‑72 (MQ=255)

                                                        agTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATaaa                                         <  2:4538581/101‑1 (MQ=255)

                                                         gTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATaaaa                                        <  1:4538758/101‑1 (MQ=255)

                                                           gCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAAtt                                      <  1:4538807/101‑1 (MQ=255)

                                                           gCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAAtt                                      <  2:4538626/101‑1 (MQ=255)

                                                            cGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTg                                     <  1:4539190/101‑1 (MQ=255)

                                                              gAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATTCCGGGCAATAAAATTGGc                                   >  1:4539353/1‑101 (MQ=255)

                                                               aaaTCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAAtt                                      >  2:4539357/1‑97 (MQ=255)

                                                               aaaTCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGcc                                  >  2:4539354/1‑101 (MQ=255)

                                                                aaTCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGa                                                                               >  1:4539361/1‑55 (MQ=255)

                                                                 aTCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCctg                                <  2:4538817/101‑1 (MQ=255)

                                                                  tCGGGTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCctgc                               >  2:4539364/1‑101 (MQ=255)

                                                                     ggTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGa                            >  1:4539367/1‑101 (MQ=255)

                                                                     ggTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGa                            <  2:4538836/101‑1 (MQ=255)

                                                                      gTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGaa                           <  1:4539075/101‑1 (MQ=255)

                                                                      gTACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGaa                           >  2:4539370/1‑101 (MQ=255)

                                                                       tACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAAt                          <  1:4539008/101‑1 (MQ=255)

                                                                       tACGCATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAAt                          <  1:4538941/101‑1 (MQ=255)

                                                                           cATTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTcc                      <  1:4538739/101‑1 (MQ=255)

                                                                            aTTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCca                     <  2:4538666/101‑1 (MQ=255)

                                                                            aTTGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCca                     <  1:4538788/101‑1 (MQ=255)

                                                                             ttGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCcat                    <  1:4539116/101‑1 (MQ=255)

                                                                             ttGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCcat                    <  1:4538854/101‑1 (MQ=255)

                                                                              tGCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCcatc                   >  2:4539373/1‑101 (MQ=255)

                                                                               gCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCcatca                  <  2:4539164/101‑1 (MQ=255)

                                                                               gCGGTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCcatca                  >  2:4539376/1‑101 (MQ=255)

                                                                                  gTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATcg               <  1:4538511/101‑1 (MQ=255)

                                                                                  gTGCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATcg               <  1:4538679/101‑1 (MQ=255)

                                                                                    gCGAGATACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATcgcg             >  1:4539378/1‑101 (MQ=255)

                                                                                       agaTACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGa          <  2:4538777/101‑1 (MQ=255)

                                                                                        gaTACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGaa         <  2:4538741/101‑1 (MQ=255)

                                                                                        gaTACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGaa         >  2:4539383/1‑101 (MQ=255)

                                                                                         aTACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAg        >  1:4539384/1‑101 (MQ=255)

                                                                                          tACCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGt       >  2:4539387/1‑101 (MQ=255)

                                                                                           aCCGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGtt      >  1:4539390/1‑101 (MQ=255)

                                                                                             cGGGCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTAt    <  2:4538989/101‑1 (MQ=255)

                                                                                              gggCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTAtt   >  1:4539395/1‑101 (MQ=255)

                                                                                              gggCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTAtt   <  1:4538967/101‑1 (MQ=255)

                                                                                              gggCGCGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTAtt   <  1:4538869/101‑1 (MQ=255)

TGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTATTG  >  NC_000913/2500065‑2500260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`