BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,597,326	G→A	A98V (GCT→GTT)	purC ←	phosphoribosylaminoimidazole‑succinocarboxamide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,597,326	0	G	A	100.0%	765.9 / NA	176	A98V (GCT→GTT)	purC	phosphoribosylaminoimidazole‑succinocarboxamide synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (91/85); total (91/85)

Only 100 of 183 total aligned reads displayed.

TCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGAT  >  NC_000913/2597228‑2597423

tCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCcaaca                                                                                                 <  2:4715247/101‑1 (MQ=255)

  ggATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCaacagc                                                                                               <  1:4715518/101‑1 (MQ=255)

  ggATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCaacagc                                                                                               <  1:4715609/101‑1 (MQ=255)

    aTCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCAc                                                                                             <  1:4715486/101‑1 (MQ=255)

    aTCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCAc                                                                                             <  2:4715436/101‑1 (MQ=255)

    aTCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCAc                                                                                             <  2:4715450/101‑1 (MQ=255)

     tCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACg                                                                                            <  1:4715567/101‑1 (MQ=255)

      cGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACgg                                                                                           <  2:4715369/101‑1 (MQ=255)

       gTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGt                                                                                          <  1:4715524/101‑1 (MQ=255)

        tGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGtt                                                                                         <  1:4715663/101‑1 (MQ=255)

            tGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGc                                                                                     <  1:4715299/101‑1 (MQ=255)

             ggCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCa                                                                                    <  1:4714929/101‑1 (MQ=255)

             ggCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCa                                                                                    <  1:4715883/101‑1 (MQ=255)

              gCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACgg                                                                                           <  1:4715605/93‑1 (MQ=255)

                 tcgtTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGac                                                                                <  2:4715891/101‑1 (MQ=255)

                    tttttCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACg                                                                                            <  2:4715344/86‑1 (MQ=255)

                     ttttCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGacacac                                                                            <  1:4715418/101‑1 (MQ=255)

                          aGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAAc                                                                       <  2:4715712/101‑1 (MQ=255)

                           ggAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAAcc                                                                      <  2:4715557/101‑1 (MQ=255)

                            gAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCg                                                                     <  1:4715598/101‑1 (MQ=255)

                            gAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCg                                                                     <  2:4715634/101‑1 (MQ=255)

                                  aTCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCa                                                               <  1:4715751/101‑1 (MQ=255)

                                   tCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCat                                                              <  1:4715613/101‑1 (MQ=255)

                                    cGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCata                                                             <  1:4715575/101‑1 (MQ=255)

                                     gAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCatat                                                            <  1:4715802/101‑1 (MQ=255)

                                      aaCAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCATGACACACTCAACCGGCACCATATc                                                           <  2:4715542/101‑1 (MQ=255)

                                            ggcggGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCtt                                                     >  2:4715994/1‑101 (MQ=255)

                                             gcggGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCttt                                                    <  2:4715574/101‑1 (MQ=255)

                                              cggGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCtttt                                                   >  1:4715997/1‑101 (MQ=255)

                                               gggTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCttttt                                                  >  2:4715999/1‑101 (MQ=255)

                                               gggTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCttttt                                                  <  1:4715550/101‑1 (MQ=255)

                                                ggTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTc                                                 >  1:4716001/1‑101 (MQ=255)

                                                 gTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCa                                                <  1:4715563/101‑1 (MQ=255)

                                                 gTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCa                                                >  2:4716002/1‑101 (MQ=255)

                                                  ttCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCAc                                               >  2:4716004/1‑101 (MQ=255)

                                                   tCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCAcc                                              >  2:4716006/1‑101 (MQ=255)

                                                     aGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAg                                            >  2:4716008/1‑101 (MQ=255)

                                                      gCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGa                                           >  1:4716010/1‑101 (MQ=255)

                                                       cTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGAc                                          >  2:4716011/1‑101 (MQ=255)

                                                       cTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGAc                                          <  1:4715527/101‑1 (MQ=255)

                                                       cTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGAc                                          >  1:4716013/1‑101 (MQ=255)

                                                         cAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACAt                                        >  1:4716017/1‑101 (MQ=255)

                                                         cAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACAt                                        >  1:4716016/1‑101 (MQ=255)

                                                          aaTACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACAtt                                       >  2:4716019/1‑101 (MQ=255)

                                                           aTACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTc                                      >  2:4716022/1‑101 (MQ=255)

                                                            tACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCg                                     >  1:4716025/1‑101 (MQ=255)

                                                            tACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCg                                     >  2:4716024/1‑101 (MQ=255)

                                                             aCCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCgg                                    >  1:4716027/1‑101 (MQ=255)

                                                             aCCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCgg                                    >  1:4716029/1‑101 (MQ=255)

                                                              ccTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCgg                                    <  1:4715596/100‑1 (MQ=255)

                                                               cttcttCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTa                                  >  1:4716031/1‑101 (MQ=255)

                                                                 tcttcGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATc                                >  2:4716033/1‑101 (MQ=255)

                                                                   ttcGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCgg                              <  1:4715772/101‑1 (MQ=255)

                                                                    tcGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGga                             >  1:4716036/1‑101 (MQ=255)

                                                                    tcGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGga                             >  2:4716035/1‑101 (MQ=255)

                                                                      gATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGgaga                           >  1:4716039/1‑101 (MQ=255)

                                                                      gATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGgaga                           >  2:4716038/1‑101 (MQ=255)

                                                                       aTTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCgg                              >  1:4716042/1‑97 (MQ=255)

                                                                        ttCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGc                         >  2:4716043/1‑101 (MQ=255)

                                                                         tCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACCCTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCa                        <  2:4715675/101‑1 (MQ=255)

                                                                         tCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCa                        <  1:4715403/101‑1 (MQ=255)

                                                                          ccAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAg                       >  1:4716048/1‑101 (MQ=255)

                                                                          ccAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAg                       >  1:4716046/1‑101 (MQ=255)

                                                                          ccAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAg                       >  2:4716049/1‑101 (MQ=255)

                                                                           cAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGa                      >  1:4716052/1‑101 (MQ=255)

                                                                            aaGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGAc                     <  1:4715709/101‑1 (MQ=255)

                                                                            aaGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGAc                     <  2:4715645/101‑1 (MQ=255)

                                                                            aaGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGAc                     <  1:4715892/101‑1 (MQ=255)

                                                                             aGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGagag                          <  1:4715795/95‑1 (MQ=255)

                                                                              gACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAAcc                                                                      >  1:4716056/1‑50 (MQ=255)

                                                                              gACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGc                   >  2:4716054/1‑101 (MQ=255)

                                                                              gACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGc                   <  2:4715769/101‑1 (MQ=255)

                                                                              gACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGc                   >  1:4716055/1‑101 (MQ=255)

                                                                              gACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATACAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGc                   <  1:4715838/101‑1 (MQ=255)

                                                                               aCGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCt                  >  2:4716058/1‑101 (MQ=255)

                                                                               aCGTTTCACCAGAGAGCCAACAGCAAGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCt                  >  1:4716059/1‑101 (MQ=255)

                                                                                 gTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTcc                >  1:4716062/1‑101 (MQ=255)

                                                                                 gTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTcc                <  1:4715585/101‑1 (MQ=255)

                                                                                  tttCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCa               >  2:4716064/1‑101 (MQ=255)

                                                                                   ttCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCAt              <  1:4715535/101‑1 (MQ=255)

                                                                                   ttCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCAt              >  1:4716065/1‑101 (MQ=255)

                                                                                    tCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCAtt             <  2:4715687/101‑1 (MQ=255)

                                                                                    tCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCAtt             >  1:4716067/1‑101 (MQ=255)

                                                                                    tCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCAtt             >  1:4716068/1‑101 (MQ=255)

                                                                                    tCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCAtt             <  2:4715664/101‑1 (MQ=255)

                                                                                      aCCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTg           >  1:4716070/1‑101 (MQ=255)

                                                                                      aCCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTg           <  2:4715618/101‑1 (MQ=255)

                                                                                      aCCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTg           <  1:4715649/101‑1 (MQ=255)

                                                                                      aCCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTg           <  2:4715742/101‑1 (MQ=255)

                                                                                        cAGAGAGCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAg         >  1:4716072/1‑101 (MQ=255)

                                                                                         agagagCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGt        >  1:4716076/1‑101 (MQ=255)

                                                                                         agagagCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGt        <  2:4715519/101‑1 (MQ=255)

                                                                                         agagagCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGt        >  2:4716075/1‑101 (MQ=255)

                                                                                          gagagCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTc       >  1:4716079/1‑101 (MQ=255)

                                                                                          gagagCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTc       <  1:4715783/101‑1 (MQ=255)

                                                                                          gagagCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTc       <  2:4715637/101‑1 (MQ=255)

                                                                                          gagagCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTc       >  2:4716080/1‑101 (MQ=255)

                                                                                           agagCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCACACGCTCCATTTGAGTCg      <  1:4715639/101‑1 (MQ=255)

                                                                                              gCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGa   >  1:4716082/1‑101 (MQ=255)

                                                                                              gCCAACAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGa   >  2:4716084/1‑101 (MQ=255)

TCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGAT  >  NC_000913/2597228‑2597423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`