Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,440,129 T→C T301A (ACT→GCT)  rpoA ← RNA polymerase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,1290TC99.7% 1648.8 / ‑4.4 387T301A (ACT→GCT) rpoARNA polymerase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (165/221);  total (166/221)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 387 total aligned reads displayed.

GTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAG  >  NC_000913/3440031‑3440226
                                                                                                  |                                                                                                 
gtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCaa                                                                                                 <  1:6417110/101‑1 (MQ=255)
 tgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAag                                                                                                >  2:6417464/1‑101 (MQ=255)
  gATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAaga                                                                                               <  2:6416980/101‑1 (MQ=255)
  gATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAaga                                                                                               <  2:6417074/101‑1 (MQ=255)
   aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAagag                                                                                              >  1:6417468/1‑101 (MQ=255)
   aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAagag                                                                                              >  2:6417471/1‑101 (MQ=255)
    tCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAgaga                                                                                             <  1:6417225/101‑1 (MQ=255)
     ccGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGAt                                                                                            <  2:6416837/101‑1 (MQ=255)
     ccGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGAt                                                                                            <  1:6416662/101‑1 (MQ=255)
      cGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGAtt                                                                                           <  2:6416914/101‑1 (MQ=255)
       ggTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGAttt                                                                                          <  1:6416818/101‑1 (MQ=255)
       ggTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGAttt                                                                                          >  1:6417478/1‑101 (MQ=255)
       ggTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGAttt                                                                                          <  1:6417159/101‑1 (MQ=255)
          tACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGAtttttt                                                                                       >  1:6417482/1‑101 (MQ=255)
           aCTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTa                                                                                      <  2:6416690/101‑1 (MQ=255)
           aCTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTa                                                                                      >  1:6417488/1‑101 (MQ=255)
            cTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGAtt                                                                                           >  1:6417493/1‑95 (MQ=255)
            cTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTAc                                                                                     <  2:6417120/101‑1 (MQ=255)
            cTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTAc                                                                                     <  1:6417172/101‑1 (MQ=255)
              cGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCa                                                                                   <  2:6417166/101‑1 (MQ=255)
              cGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCa                                                                                   >  2:6417494/1‑101 (MQ=255)
               gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCaa                                                                                  <  2:6417088/101‑1 (MQ=255)
               gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCaa                                                                                  >  1:6417500/1‑101 (MQ=255)
                tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAg                                                                                 >  1:6417505/1‑101 (MQ=255)
                tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAg                                                                                 <  2:6416562/101‑1 (MQ=255)
                 cAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAgg                                                                                <  1:6416537/101‑1 (MQ=255)
                 cAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAgg                                                                                >  2:6417509/1‑101 (MQ=255)
                  aGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGt                                                                               >  1:6417510/1‑101 (MQ=255)
                   gCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGtt                                                                              <  2:6416628/101‑1 (MQ=255)
                   gCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGtt                                                                              >  2:6417515/1‑101 (MQ=255)
                   gCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGtt                                                                              <  1:6416751/101‑1 (MQ=255)
                    cGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTa                                                                             <  2:6417054/101‑1 (MQ=255)
                    cGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTa                                                                             <  1:6417124/101‑1 (MQ=255)
                        gCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCg                                                                         <  2:6417142/101‑1 (MQ=255)
                        gCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCg                                                                         <  1:6416778/101‑1 (MQ=255)
                        gCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCg                                                                         <  1:6416310/101‑1 (MQ=255)
                          ttGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGtt                                                                       <  2:6416767/101‑1 (MQ=255)
                           tGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGttt                                                                      >  2:6417520/1‑101 (MQ=255)
                             ccGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTa                                                                    <  2:6416867/101‑1 (MQ=255)
                              cGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCa                                                                                   >  2:6417524/1‑85 (MQ=255)
                              cGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                   <  2:6416740/101‑1 (MQ=255)
                               ggTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                  >  1:6417525/1‑101 (MQ=255)
                                gTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGtttt                                                                     <  2:6417151/97‑1 (MQ=255)
                                    ccAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCt                                                             >  2:6417529/1‑101 (MQ=255)
                                       gTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCaa                                                          <  1:6417081/101‑1 (MQ=255)
                                         tttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAcc                                                        >  1:6417537/1‑101 (MQ=255)
                                         tttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAcc                                                        >  2:6417536/1‑101 (MQ=255)
                                            ccAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                     <  2:6416803/101‑1 (MQ=255)
                                            ccAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                     >  1:6417541/1‑101 (MQ=255)
                                             cAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCgg                                                    <  1:6416898/101‑1 (MQ=255)
                                               ggCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTa                                                  >  2:6417546/1‑101 (MQ=255)
                                                gcgcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTAc                                                 <  2:6417250/101‑1 (MQ=255)
                                                 cgcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                <  2:6416855/101‑1 (MQ=255)
                                                  gcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGc                                               <  1:6416948/101‑1 (MQ=255)
                                                  gcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGc                                               >  2:6417552/1‑101 (MQ=255)
                                                    aTGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGAtttttt                                                                                       <  2:6416848/59‑1 (MQ=255)
                                                    aTGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTg                                             >  1:6417553/1‑101 (MQ=255)
                                                    aTGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTg                                             <  2:6416938/101‑1 (MQ=255)
                                                        ccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTAcc                                         <  2:6417034/101‑1 (MQ=255)
                                                        ccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAACTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTAcc                                         >  1:6417558/1‑101 (MQ=255)
                                                          agagACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAg                                       >  1:6417562/1‑101 (MQ=255)
                                                            agaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGAt                                     <  2:6416824/101‑1 (MQ=255)
                                                            agaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGAt                                     >  2:6417566/1‑101 (MQ=255)
                                                             gaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATc                                    >  1:6417568/1‑101 (MQ=255)
                                                               cAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAc                                  <  2:6417107/101‑1 (MQ=255)
                                                                aGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAcc                                 >  1:6417575/1‑101 (MQ=255)
                                                                aGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAcc                                 >  2:6417574/1‑101 (MQ=255)
                                                                   ccACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGat                              >  1:6417580/1‑101 (MQ=255)
                                                                     aCGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGatat                            >  2:6417586/1‑101 (MQ=255)
                                                                     aCGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGatat                            >  1:6417587/1‑101 (MQ=255)
                                                                     aCGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGatat                            <  1:6416874/101‑1 (MQ=255)
                                                                      cGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGAtata                           <  2:6417005/101‑1 (MQ=255)
                                                                       gggAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAg                          <  1:6417065/101‑1 (MQ=255)
                                                                        ggAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGt                         >  1:6417591/1‑101 (MQ=255)
                                                                         gAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTg                        <  2:6417483/101‑1 (MQ=255)
                                                                          aaGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTgg                       <  1:6417197/101‑1 (MQ=255)
                                                                           aGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCt                                              <  1:6416999/77‑1 (MQ=255)
                                                                            gCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGAt                     <  1:6416723/101‑1 (MQ=255)
                                                                            gCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGAt                     <  2:6416887/101‑1 (MQ=255)
                                                                             ccAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATa                    >  2:6417597/1‑101 (MQ=255)
                                                                             ccAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATa                    <  1:6417031/101‑1 (MQ=255)
                                                                               aGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGc                  <  1:6417090/101‑1 (MQ=255)
                                                                               aGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGc                  >  1:6417599/1‑101 (MQ=255)
                                                                                 cACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCtt                <  2:6416954/101‑1 (MQ=255)
                                                                                  aCGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc               <  1:6417371/101‑1 (MQ=255)
                                                                                  aCGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc               <  1:6417256/101‑1 (MQ=255)
                                                                                   cGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCt              <  2:6417064/101‑1 (MQ=255)
                                                                                    gTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg             >  2:6417607/1‑101 (MQ=255)
                                                                                    gTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg             <  1:6416976/101‑1 (MQ=255)
                                                                                    gTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg             <  1:6416925/101‑1 (MQ=255)
                                                                                    gTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg             >  1:6417605/1‑101 (MQ=255)
                                                                                      cTTTAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCt           >  2:6417612/1‑101 (MQ=255)
                                                                                       tttAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCtt          <  1:6417047/101‑1 (MQ=255)
                                                                                         tAATCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTa        >  1:6417617/1‑101 (MQ=255)
                                                                                          aaTCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTaa       <  2:6416894/101‑1 (MQ=255)
                                                                                          aaTCTCAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTaa       >  2:6417620/1‑101 (MQ=255)
                                                                                             ctcAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGa                                                                <  1:6416849/41‑1 (MQ=255)
                                                                                             ctcAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGc    <  2:6416969/101‑1 (MQ=255)
                                                                                             ctcAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGc    >  1:6417625/1‑101 (MQ=255)
                                                                                              tcAGCAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa   >  2:6417626/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAG  >  NC_000913/3440031‑3440226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑