BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,543	(C)_8→7	coding (492/732 nt)	pgeF ←	polyphenol oxidase YfiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,536	0	C	.	99.0%	1622.1 / 5.2	314	coding (499/732 nt)	pgeF	polyphenol oxidase YfiH
Reads supporting (aligned to +/- strand): ref base C (1/2); new base . (161/150); total (162/152)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 342 total aligned reads displayed.

TGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAG  >  NC_000913/2734436‑2734632

tGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTc                                                                                                  >  1:4925509/1‑101 (MQ=255)

    gggCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTccccc                                                                                              >  1:4925516/1‑101 (MQ=255)

     ggCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTcccccc                                                                                             <  1:4924917/101‑1 (MQ=255)

      gCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTccccccc                                                                                            >  2:4925518/1‑101 (MQ=255)

        aaGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTcccccccac                                                                                          <  1:4925000/101‑3 (MQ=255)

         aGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTcccccccact                                                                                         >  2:4925522/1‑98 (MQ=255)

           cTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTc                                                                                      >  2:4925526/1‑101 (MQ=255)

              aTAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGaa                                                                                   >  1:4925530/1‑101 (MQ=255)

               tAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTcccccccac                                                                                          >  2:4925534/1‑92 (MQ=255)

               tAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAAc                                                                                  <  1:4924980/101‑1 (MQ=255)

                aaaTATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAAcg                                                                                 >  1:4925536/1‑101 (MQ=255)

                 aaTATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAAcgc                                                                                >  2:4925538/1‑101 (MQ=255)

                 aaTATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAAcgc                                                                                <  1:4924495/101‑1 (MQ=255)

                 aaTATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAAcgc                                                                                <  2:4924907/101‑1 (MQ=255)

                   tatCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAAcgcgc                                                                              <  2:4925012/101‑1 (MQ=255)

                    atCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAAcgcgcg                                                                             >  1:4925543/1‑101 (MQ=255)

                     tCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGt                                                                            >  1:4925547/1‑101 (MQ=255)

                        gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGa                                                                         >  2:4925552/1‑101 (MQ=255)

                        gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGa                                                                         >  1:4925551/1‑101 (MQ=255)

                           aGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCa                                                                      >  1:4925557/1‑101 (MQ=255)

                            gATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCaa                                                                     <  1:4925055/101‑1 (MQ=255)

                            gATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCaa                                                                     >  1:4925561/1‑101 (MQ=255)

                               aCTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTg                                                                  <  2:4925086/101‑1 (MQ=255)

                                 ttATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGcc                                                                >  2:4925568/1‑101 (MQ=255)

                                 ttATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGcc                                                                <  2:4925154/101‑1 (MQ=255)

                                    tCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGc                                                             <  2:4925235/101‑1 (MQ=255)

                                    tCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTACCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGc                                                             <  2:4925323/101‑1 (MQ=255)

                                     cACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc                                                            >  1:4925572/1‑101 (MQ=255)

                                      aCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGccc                                                           >  2:4925575/1‑101 (MQ=255)

                                       ccATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCt                                                          <  2:4925210/101‑1 (MQ=255)

                                         aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTaa                                                        <  2:4925033/101‑1 (MQ=255)

                                         aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTaa                                                        >  2:4925581/1‑101 (MQ=255)

                                         aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTaa                                                        >  1:4925579/1‑101 (MQ=255)

                                           gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAcc                                                      >  1:4925586/1‑101 (MQ=255)

                                            cTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCa                                                     >  2:4925589/1‑101 (MQ=255)

                                             tgaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAg                                                    >  2:4925593/1‑101 (MQ=255)

                                             tgaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAg                                                    <  1:4925073/101‑1 (MQ=255)

                                             tgaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAg                                                    >  1:4925594/1‑101 (MQ=255)

                                               aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGc                                                  >  1:4925599/1‑101 (MQ=255)

                                                atgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCg                                                 <  2:4925118/101‑1 (MQ=255)

                                                 tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCga                                                >  2:4925604/1‑101 (MQ=255)

                                                 tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCga                                                <  1:4925137/101‑1 (MQ=255)

                                                   aaaGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCgaga                                              >  2:4925607/1‑101 (MQ=255)

                                                    aaGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGaa                                             >  1:4925610/1‑101 (MQ=255)

                                                      gCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAata                                           <  1:4925237/101‑1 (MQ=255)

                                                       cTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAatat                                          <  2:4925346/101‑1 (MQ=255)

                                                       cTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAatat                                          >  1:4925614/1‑101 (MQ=255)

                                                        tGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATAtt                                         >  2:4925617/1‑101 (MQ=255)

                                                         gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATAttt                                        <  2:4925131/101‑1 (MQ=255)

                                                         gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATAttt                                        <  1:4925017/101‑1 (MQ=255)

                                                         gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATAttt                                        <  2:4924987/101‑1 (MQ=255)

                                                          cACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATAtttt                                       >  1:4925620/1‑101 (MQ=255)

                                                           aCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTg                                                                           >  1:4925626/1‑64 (MQ=255)

                                                           aCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTc                                      >  2:4925624/1‑101 (MQ=255)

                                                            cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTcc                                     <  2:4924962/101‑1 (MQ=255)

                                                            cTTGCTGTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTcc                                     <  1:4925314/101‑1 (MQ=255)

                                                             ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATAtttt                                       <  1:4925029/98‑1 (MQ=255)

                                                             ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCg                                    <  2:4925070/101‑1 (MQ=255)

                                                              tGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCgg                                   >  1:4925629/1‑101 (MQ=255)

                                                               gCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGa                                  <  2:4925043/101‑1 (MQ=255)

                                                                cTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTcc                                     <  2:4925101/97‑1 (MQ=255)

                                                                 tttAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCATGCGAGAATATTTTCCGGAtt                                >  1:4925637/1‑101 (MQ=255)

                                                                 tttAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGAtt                                >  1:4925634/1‑101 (MQ=255)

                                                                  ttAGCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTa                               >  1:4925641/1‑101 (MQ=255)

                                                                     gCGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCa                            <  2:4925264/101‑1 (MQ=255)

                                                                      cGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAg                           <  1:4925189/101‑1 (MQ=255)

                                                                      cGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAg                           <  1:4925109/101‑1 (MQ=255)

                                                                      cGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAg                           >  2:4925645/1‑101 (MQ=255)

                                                                      cGTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAg                           <  2:4924833/101‑1 (MQ=255)

                                                                       gTCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGc                          <  2:4925058/101‑1 (MQ=255)

                                                                        tCTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa                         >  2:4925649/1‑101 (MQ=255)

                                                                         cTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaa                        >  1:4925651/1‑101 (MQ=255)

                                                                         cTACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaa                        <  1:4925225/101‑1 (MQ=255)

                                                                          tACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaaa                       >  1:4925656/1‑101 (MQ=255)

                                                                          tACTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaaa                       >  2:4925655/1‑101 (MQ=255)

                                                                           aCTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaaaa                      <  1:4925091/101‑1 (MQ=255)

                                                                            cTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAAcgc                                                                                >  1:4925663/1‑42 (MQ=39)

                                                                            cTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAc                     <  2:4925052/101‑1 (MQ=255)

                                                                            cTGCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAc                     <  1:4924953/101‑1 (MQ=255)

                                                                              gCCATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg                   <  1:4925410/101‑1 (MQ=255)

                                                                               ccATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg                  <  2:4925361/101‑1 (MQ=255)

                                                                                cATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                 <  2:4925190/101‑1 (MQ=255)

                                                                                cATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                 <  2:4925271/101‑1 (MQ=255)

                                                                                cATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                 <  2:4925180/101‑1 (MQ=255)

                                                                                cATAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                 >  2:4925665/1‑101 (MQ=255)

                                                                                 aTAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaa                <  2:4925511/101‑1 (MQ=255)

                                                                                  tAAACGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa               >  1:4925668/1‑101 (MQ=255)

                                                                                     aCGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCg            <  1:4924890/101‑1 (MQ=255)

                                                                                     aCGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCg            <  1:4925244/101‑1 (MQ=255)

                                                                                     aCGCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCg            <  1:4925167/101‑1 (MQ=255)

                                                                                       gCCTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtc          >  2:4925671/1‑101 (MQ=255)

                                                                                        ccTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtct         <  2:4925007/101‑1 (MQ=255)

                                                                                         cTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg                  >  1:4925677/1‑91 (MQ=255)

                                                                                         cTCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtctc        <  2:4925021/101‑1 (MQ=255)

                                                                                          tCGCGAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTctct       >  2:4925680/1‑101 (MQ=255)

                                                                                           cgcgAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCtt      <  1:4925217/101‑1 (MQ=255)

                                                                                            gcgAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTc     >  2:4925683/1‑101 (MQ=255)

                                                                                            gcgAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTc     <  1:4925157/101‑1 (MQ=255)

                                                                                              gAACCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCa   >  1:4925684/1‑101 (MQ=255)

                                                                                               aaCCT‑CCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAg  >  1:4925691/1‑101 (MQ=255)

TGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAG  >  NC_000913/2734436‑2734632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`