BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,727,494	(G)_6→7	coding (836/1098 nt)	nemA →	N‑ethylmaleimide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,727,488	1	.	G	100.0%	1023.9 / NA	244	A277G (GCG→GGG)	nemA	N‑ethylmaleimide reductase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (146/98); total (146/98)

Only 100 of 263 total aligned reads displayed.

ACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAG  >  NC_000913/1727388‑1727583

    aTAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑gggg                                                                                             >  2:3120672/1‑101 (MQ=255)

      aaCGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑ggtggg                                                                                           >  1:3120675/1‑101 (MQ=255)

       aCGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑ggggggg                                                                                          >  2:3120677/1‑100 (MQ=255)

         ggCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑gggggggtg                                                                                        <  2:3120149/101‑4 (MQ=255)

           cccGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑gggggggtgaa                                                                                      >  2:3120680/1‑96 (MQ=255)

             cGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAAcc                                                                                     <  2:3120439/101‑1 (MQ=255)

             cGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAAcc                                                                                     >  2:3120683/1‑101 (MQ=255)

              gAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCg                                                                                    >  1:3120685/1‑101 (MQ=255)

                aTGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGt                                                                                   >  2:3120690/1‑101 (MQ=255)

                aTGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGta                                                                                  <  1:3120195/101‑1 (MQ=255)

                aTGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGta                                                                                  <  2:3120279/101‑1 (MQ=255)

                 tGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGta                                                                                  <  1:3119910/101‑1 (MQ=255)

                 tGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGta                                                                                  >  1:3120693/1‑101 (MQ=255)

                 tGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGta                                                                                  >  2:3120694/1‑101 (MQ=255)

                   aagaagCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATAc                                                                               >  2:3120698/1‑101 (MQ=255)

                    agaagCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACt                                                                              >  1:3120701/1‑101 (MQ=255)

                    agaagCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACt                                                                              <  1:3120352/101‑1 (MQ=255)

                     gaagCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTg                                                                             <  2:3120408/101‑1 (MQ=255)

                       agCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGAt                                                                           >  2:3120705/1‑101 (MQ=255)

                       agCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGAt                                                                           >  1:3120707/1‑101 (MQ=255)

                        gCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATg                                                                          >  2:3120709/1‑101 (MQ=255)

                         ccGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGc                                                                         <  1:3120357/101‑1 (MQ=255)

                         ccGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGc                                                                         <  2:3120264/101‑1 (MQ=255)

                          cGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCg                                                                        >  2:3120713/1‑101 (MQ=255)

                          cGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCg                                                                        <  2:3120459/101‑1 (MQ=255)

                           gATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGt                                                                       >  1:3120715/1‑101 (MQ=255)

                            aTGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGtt                                                                      >  1:3120717/1‑101 (MQ=255)

                             tGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTc                                                                     >  1:3120720/1‑101 (MQ=255)

                              gCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTcc                                                                    <  1:3120332/101‑1 (MQ=255)

                               cACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTcc                                                                    >  1:3120722/1‑101 (MQ=255)

                                aCTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCcgc                                                                  <  1:3120466/101‑1 (MQ=255)

                                  tGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGa                                                                >  2:3120725/1‑101 (MQ=255)

                                   gTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGAGAACCGTATACTGATGCGTTCCGCGaa                                                               >  2:3120727/1‑101 (MQ=255)

                                    tATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGaaa                                                              <  1:3120328/101‑1 (MQ=255)

                                     aTCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGaaaa                                                             >  1:3120729/1‑101 (MQ=255)

                                      tCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGaaaaa                                                            <  1:3120266/101‑1 (MQ=255)

                                       cTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAg                                                           >  1:3120734/1‑101 (MQ=255)

                                       cTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAg                                                           <  2:3120059/101‑1 (MQ=255)

                                        tGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGt                                                          <  2:3120137/101‑1 (MQ=255)

                                        tGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGt                                                          >  2:3120736/1‑101 (MQ=255)

                                         gATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa                                                         <  2:3120293/101‑1 (MQ=255)

                                          aTTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAc                                                        <  2:3120227/101‑1 (MQ=255)

                                           ttGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAc                                                        >  1:3120739/1‑101 (MQ=255)

                                           ttGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcg                                                       <  2:3120319/101‑1 (MQ=255)

                                            tGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgc                                                      >  2:3120740/1‑101 (MQ=255)

                                            tGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgc                                                      <  1:3120184/101‑1 (MQ=255)

                                             gAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgcg                                                     >  2:3120743/1‑101 (MQ=255)

                                             gAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgcg                                                     <  2:3120300/101‑1 (MQ=255)

                                                caacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGccc                                                  >  1:3120747/1‑101 (MQ=255)

                                                caacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGcc                                                   >  2:3120749/1‑100 (MQ=255)

                                                 aacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGccc                                                  >  2:3120751/1‑101 (MQ=255)

                                                  acTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGt                                                >  2:3120754/1‑101 (MQ=255)

                                                     gggTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTc                                             >  1:3120758/1‑101 (MQ=255)

                                                     gggTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTc                                             >  2:3120759/1‑101 (MQ=255)

                                                      ggTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTcc                                            <  1:3120308/101‑1 (MQ=255)

                                                       gTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTcc                                            >  1:3120762/1‑101 (MQ=255)

                                                        tAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGccc                                                  >  2:3120763/1‑93 (MQ=255)

                                                          aaCGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACgg                                        >  2:3120767/1‑101 (MQ=255)

                                                             gcgGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTcc                                     <  1:3120254/101‑1 (MQ=255)

                                                             gcgGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTcc                                     >  2:3120771/1‑101 (MQ=255)

                                                              cgGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCg                                    >  2:3120773/1‑101 (MQ=255)

                                                                 cATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGAtt                                 >  2:3120776/1‑101 (MQ=255)

                                                                 cATTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGAtt                                 >  1:3120777/1‑101 (MQ=255)

                                                                  aTTGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTa                                >  1:3120780/1‑101 (MQ=255)

                                                                   ttGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTAt                               >  2:3120782/1‑101 (MQ=255)

                                                                   ttGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTAt                               <  2:3120376/101‑1 (MQ=255)

                                                                    tGCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATc                              <  1:3120277/101‑1 (MQ=255)

                                                                     gCTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCg                             >  2:3120785/1‑101 (MQ=255)

                                                                      cTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCgg                            <  2:3120531/101‑1 (MQ=255)

                                                                      cTTATCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCgg                            >  1:3120787/1‑101 (MQ=255)

                                                                         aTCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcgc                         >  1:3120791/1‑101 (MQ=255)

                                                                         aTCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcgc                         <  1:3120348/101‑1 (MQ=255)

                                                                          tCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                        >  1:3120794/1‑101 (MQ=255)

                                                                          tCTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                        <  1:3120481/101‑1 (MQ=255)

                                                                           cTGCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAg                       <  2:3120193/101‑1 (MQ=255)

                                                                             gCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGt                     >  2:3120797/1‑101 (MQ=255)

                                                                             gCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGt                     <  1:3120579/101‑1 (MQ=255)

                                                                             gCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGt                     <  2:3120395/101‑1 (MQ=255)

                                                                             gCATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGt                     >  1:3120799/1‑101 (MQ=255)

                                                                              cATATGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTg                    >  2:3120801/1‑101 (MQ=255)

                                                                               atatGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa                                                         <  1:3120314/64‑1 (MQ=255)

                                                                                tatGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCa                  >  1:3120804/1‑101 (MQ=255)

                                                                                 atGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                        >  2:3120807/1‑94 (MQ=255)

                                                                                 atGTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCAt                 <  1:3120423/101‑1 (MQ=255)

                                                                                   gTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATac               <  1:3120147/101‑1 (MQ=255)

                                                                                   gTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATac               >  1:3120808/1‑101 (MQ=255)

                                                                                   gTCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATac               <  1:3120412/101‑1 (MQ=255)

                                                                                    tCAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATaca              <  2:3120661/101‑1 (MQ=255)

                                                                                     cAGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac             >  1:3120811/1‑101 (MQ=255)

                                                                                      aGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTg                                                                             <  1:3120239/37‑1 (MQ=255)

                                                                                      aGAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATAcaca            >  2:3120815/1‑101 (MQ=255)

                                                                                       gAACCAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAg           >  1:3120816/1‑101 (MQ=255)

                                                                                           cAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGa       <  2:3120347/101‑1 (MQ=255)

                                                                                           cAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGa       >  1:3120821/1‑101 (MQ=255)

                                                                                           cAGATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGa       >  2:3120820/1‑101 (MQ=255)

                                                                                             gATTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGaaa     <  1:3120440/101‑1 (MQ=255)

                                                                                              aTTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGaaaa    >  1:3120825/1‑101 (MQ=255)

                                                                                              aTTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGaaaa    <  1:3120396/101‑1 (MQ=255)

                                                                                              aTTGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGaaaa    >  2:3120828/1‑101 (MQ=255)

                                                                                                tGGGCG‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGTGCAGGTGCATACACAGTAGAAAAAg  <  2:3120361/101‑1 (MQ=255)

ACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAG  >  NC_000913/1727388‑1727583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`