BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,471,154	A→G	S98S (AGT→AGC)	pyrI ←	aspartate carbamoyltransferase, PyrI subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,471,154	0	A	G	100.0%	1365.1 / NA	331	S98S (AGT→AGC)	pyrI	aspartate carbamoyltransferase, PyrI subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (142/189); total (142/189)

Only 100 of 331 total aligned reads displayed.

CGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCA  >  NC_000913/4471057‑4471251

 gcgTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTg                                                                                               <  1:8254113/101‑1 (MQ=255)

  cgTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTgg                                                                                              >  2:8254929/1‑101 (MQ=255)

   gTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTggg                                                                                             >  1:8254931/1‑101 (MQ=255)

    ttttCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGgc                                                                                            <  2:8254500/101‑1 (MQ=255)

     tttCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGgcg                                                                                           >  2:8254934/1‑101 (MQ=255)

      ttCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGgcgc                                                                                          <  2:8254727/101‑1 (MQ=255)

        cGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGa                                                                                        >  1:8254939/1‑101 (MQ=255)

        cGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGa                                                                                        >  2:8254940/1‑101 (MQ=255)

          cACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGAtt                                                                                      <  1:8254760/101‑1 (MQ=255)

             ggCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTAc                                                                                   >  1:8254947/1‑101 (MQ=255)

               cAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTAccc                                                                                 <  1:8254614/101‑1 (MQ=255)

                aaaGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACcca                                                                                <  2:8254659/101‑1 (MQ=255)

                aaaGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACcca                                                                                >  2:8254951/1‑101 (MQ=255)

                aaaGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACcca                                                                                >  1:8254950/1‑101 (MQ=255)

                  aGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACccacc                                                                              <  1:8254236/101‑1 (MQ=255)

                  aGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACccacc                                                                              <  1:8254174/101‑1 (MQ=255)

                   gCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACccacca                                                                             <  1:8254371/101‑1 (MQ=255)

                     tGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACt                                                                           >  1:8254954/1‑101 (MQ=255)

                      ggATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACtt                                                                          <  1:8254505/101‑1 (MQ=255)

                       gATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTc                                                                         >  1:8254959/1‑101 (MQ=255)

                        aTGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCa                                                                        >  2:8254961/1‑101 (MQ=255)

                        aTGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCa                                                                        >  1:8254964/1‑101 (MQ=255)

                          gAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATa                                                                      >  1:8254967/1‑101 (MQ=255)

                          gAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATa                                                                      <  1:8254252/101‑1 (MQ=255)

                           aaaCCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAg                                                                     <  1:8254587/101‑1 (MQ=255)

                           aaaCCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAg                                                                     <  1:8253972/101‑1 (MQ=255)

                            aaCCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGt                                                                    <  2:8254391/101‑1 (MQ=255)

                             aCCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGtt                                                                   <  1:8254415/101‑1 (MQ=255)

                               cGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGt                                                                 >  2:8254976/1‑101 (MQ=255)

                                ggTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTc                                                                <  2:8253423/101‑1 (MQ=255)

                                 gTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCg                                                               >  1:8254977/1‑101 (MQ=255)

                                  ttCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGa                                                              >  1:8254981/1‑101 (MQ=255)

                                   tCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGAt                                                             <  2:8254324/101‑1 (MQ=255)

                                   tCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGAt                                                             <  2:8254465/101‑1 (MQ=255)

                                    cGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATa                                                            >  1:8254985/1‑101 (MQ=255)

                                      gCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGgcgc                                                                                          >  2:8254988/1‑69 (MQ=255)

                                      gCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACg                                                          <  2:8254445/101‑1 (MQ=255)

                                      gCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACg                                                          <  1:8254310/101‑1 (MQ=255)

                                      gCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACg                                                          <  2:8254624/101‑1 (MQ=255)

                                        aTGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGt                                                        <  2:8254264/101‑1 (MQ=255)

                                         tgacTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGtt                                                       >  1:8254994/4‑101 (MQ=255)

                                         tGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGtt                                                       <  2:8254165/101‑1 (MQ=255)

                                         tGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGtt                                                       <  1:8254409/101‑1 (MQ=255)

                                         tGGCTGATACAGTTGCTGTTCAGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTg                                                                  >  2:8254997/1‑90 (MQ=255)

                                           gCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCg                                                               >  1:8254998/1‑91 (MQ=255)

                                           gCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTaa                                                     <  2:8254315/101‑1 (MQ=255)

                                             tGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAAcc                                                   >  1:8255002/1‑101 (MQ=255)

                                              gATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCg                                                  <  2:8254553/101‑1 (MQ=255)

                                               aTACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGt                                                 >  2:8255004/1‑101 (MQ=255)

                                               aTACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGt                                                 <  2:8254703/101‑1 (MQ=255)

                                                tACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTg                                                <  1:8254381/101‑1 (MQ=255)

                                                  cAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGc                                              >  1:8255009/1‑101 (MQ=255)

                                                    gTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCtt                                            >  2:8255013/1‑101 (MQ=255)

                                                    gTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCtt                                            <  1:8254388/101‑1 (MQ=255)

                                                     ttGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTg                                           >  1:8255014/1‑101 (MQ=255)

                                                      tGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgc                                          <  2:8254290/101‑1 (MQ=255)

                                                      tGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgc                                          <  2:8254608/101‑1 (MQ=255)

                                                      tGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgc                                          >  1:8255017/1‑101 (MQ=255)

                                                      tGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTg                                           <  2:8254335/100‑1 (MQ=255)

                                                       gCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgcg                                         <  2:8254190/101‑1 (MQ=255)

                                                        cTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgcgg                                        <  2:8254588/101‑1 (MQ=255)

                                                         tGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgcggc                                       <  1:8254660/101‑1 (MQ=255)

                                                            tCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCa                                    <  1:8254456/101‑1 (MQ=255)

                                                            tCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCa                                    >  2:8255023/1‑101 (MQ=255)

                                                            tCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCa                                    >  1:8255024/1‑101 (MQ=255)

                                                             cGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCAt                                   <  2:8254493/101‑1 (MQ=255)

                                                                gCAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACa                                <  1:8254203/101‑1 (MQ=255)

                                                                 cAGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACaa                               >  2:8255029/1‑101 (MQ=255)

                                                                  aGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgcg                                         >  1:8255031/1‑90 (MQ=255)

                                                                  aGACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAAt                              <  2:8254305/101‑1 (MQ=255)

                                                                   gACCAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgc                                          >  2:8255034/1‑88 (MQ=255)

                                                                      cAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACtt                                                                          >  2:8255041/1‑53 (MQ=255)

                                                                      cAGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCa                          >  1:8255037/1‑101 (MQ=255)

                                                                       aGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAg                         <  2:8254403/101‑1 (MQ=255)

                                                                       aGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAg                         <  2:8254758/101‑1 (MQ=255)

                                                                       aGCACATTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAg                         <  1:8254538/101‑1 (MQ=255)

                                                                          acaTTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTg                      >  1:8255046/1‑101 (MQ=255)

                                                                           caTTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGgcgc                                     >  2:8255049/1‑85 (MQ=255)

                                                                           caTTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGa                     <  1:8254425/101‑1 (MQ=255)

                                                                           caTTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGa                     <  1:8254280/101‑1 (MQ=255)

                                                                            aTTGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGAt                    <  1:8254321/101‑1 (MQ=255)

                                                                             ttGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAg                         >  2:8255053/1‑95 (MQ=255)

                                                                             ttGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATc                   <  2:8254359/101‑1 (MQ=255)

                                                                             ttGTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATc                   <  1:8254898/101‑1 (MQ=255)

                                                                               gTCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTa                 <  2:8254514/101‑1 (MQ=255)

                                                                                tCGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTAc                >  1:8255057/1‑101 (MQ=255)

                                                                                 cGATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACt               >  2:8255059/1‑101 (MQ=255)

                                                                                  gATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgcggcg                                      >  1:8255061/1‑77 (MQ=255)

                                                                                  gATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACtt              <  2:8254542/101‑1 (MQ=255)

                                                                                  gATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACtt              <  2:8254529/101‑1 (MQ=255)

                                                                                  gATGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACtt              <  2:8254397/101‑1 (MQ=255)

                                                                                   aTGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTg             <  2:8254429/101‑1 (MQ=255)

                                                                                   aTGCGCTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTg             <  1:8254484/101‑1 (MQ=255)

                                                                                        cTCCGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCtt        >  2:8255064/1‑101 (MQ=255)

                                                                                          ccGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgcg                                         >  2:8255067/1‑66 (MQ=255)

                                                                                          ccGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCa      <  1:8254574/101‑1 (MQ=255)

                                                                                           cGGCAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCAc     <  1:8254430/101‑1 (MQ=255)

                                                                                              cAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCa  <  2:8254370/101‑1 (MQ=255)

                                                                                              cAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCa  <  1:8254496/101‑1 (MQ=255)

                                                                                              cAGGCTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCa  <  1:8254342/101‑1 (MQ=255)

CGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCA  >  NC_000913/4471057‑4471251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`