Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,353,837 T→C I228V (ATT→GTT)  sapC ← putrescine ABC exporter membrane protein SapC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,353,8370TC90.6% 1000.0 / 75.5 297I228V (ATT→GTT) sapCputrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand):  ref base T (15/13);  new base C (111/158);  total (126/171)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 297 total aligned reads displayed.

ATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCA  >  NC_000913/1353739‑1353935
                                                                                                  |                                                                                                  
aTAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTg                                                                                                  <  1:2478896/101‑1 (MQ=255)
 tAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGc                                                                                                 <  2:2478778/101‑1 (MQ=255)
  aaaTCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGcc                                                                                                >  1:2479172/1‑101 (MQ=255)
   aaTCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCa                                                                                               >  2:2479173/1‑101 (MQ=255)
     tCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATc                                                                                             <  2:2478644/101‑1 (MQ=255)
     tCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATc                                                                                             <  1:2478763/101‑1 (MQ=255)
       aGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCg                                                                                            >  1:2479179/1‑100 (MQ=255)
       aGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGa                                                                                           >  2:2479178/1‑101 (MQ=255)
       aGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGa                                                                                           <  1:2478505/101‑1 (MQ=255)
         ttCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACa                                                                                         <  2:2478537/101‑1 (MQ=255)
          tCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAg                                                                                        <  1:2478619/101‑1 (MQ=255)
          tCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAg                                                                                        >  1:2479185/1‑101 (MQ=255)
            cAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTg                                                                                      >  2:2479187/1‑101 (MQ=255)
              gcgcATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTgcg                                                                                    <  1:2478596/101‑1 (MQ=255)
               cgcATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTgcgc                                                                                   >  2:2479193/1‑101 (MQ=255)
                  aTCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCggg                                                                                <  1:2478349/101‑1 (MQ=255)
                  aTCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCggg                                                                                <  1:2478733/101‑1 (MQ=255)
                  aTCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCggg                                                                                >  1:2479196/1‑101 (MQ=255)
                    cACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTg                                                                              <  2:2478769/101‑1 (MQ=255)
                     aCCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGa                                                                             >  2:2479199/1‑101 (MQ=255)
                      ccGAGCATCGCTCCCCATTCAGGCGAGGTGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGAt                                                                            <  1:2478722/101‑1 (MQ=255)
                      ccGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGAt                                                                            >  1:2479203/1‑101 (MQ=255)
                      ccGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGAt                                                                            >  2:2479202/1‑101 (MQ=255)
                        gAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGAtct                                                                          >  2:2479206/1‑101 (MQ=255)
                         aGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGAtctc                                                                         <  1:2478522/101‑1 (MQ=255)
                          gCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCg                                                                        <  1:2478682/101‑1 (MQ=255)
                          gCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCg                                                                        >  2:2479209/1‑101 (MQ=255)
                           cATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCgg                                                                       >  1:2479213/1‑101 (MQ=255)
                             tCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTg                                                                     >  2:2479215/1‑101 (MQ=255)
                              cGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGa                                                                    <  1:2478719/101‑1 (MQ=255)
                               gCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGAc                                                                   <  2:2478665/101‑1 (MQ=255)
                               gCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGAc                                                                   >  1:2479219/1‑101 (MQ=255)
                                 tCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCa                                                                 <  2:2478886/101‑1 (MQ=255)
                                  ccccATTCAGGCGAGGGGCGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTgcg                                                                                    >  2:2479225/1‑81 (MQ=255)
                                  ccccATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAg                                                                >  1:2479224/1‑101 (MQ=255)
                                  ccccATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAg                                                                <  1:2478746/101‑1 (MQ=255)
                                   cccATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGc                                                               <  2:2478479/101‑1 (MQ=255)
                                     cATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGccc                                                             <  1:2478690/101‑1 (MQ=255)
                                     cATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGccc                                                             <  2:2478587/101‑1 (MQ=255)
                                     cATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGcc                                                              <  1:2478629/100‑1 (MQ=255)
                                      aTTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCcg                                                            <  1:2478779/101‑1 (MQ=255)
                                       ttCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCcgg                                                           <  1:2478843/101‑1 (MQ=255)
                                        tCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCcg                                                            >  2:2479230/1‑99 (MQ=255)
                                         cgggCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCcggcg                                                         >  1:2479232/3‑101 (MQ=255)
                                         cAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCcggcg                                                         <  1:2478982/101‑1 (MQ=255)
                                          aGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCcggcgg                                                        >  2:2479234/1‑101 (MQ=255)
                                             cGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGa                                                     <  1:2478327/101‑1 (MQ=255)
                                              gAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGAt                                                    >  2:2479239/1‑101 (MQ=255)
                                              gAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGAt                                                    >  1:2479240/1‑101 (MQ=255)
                                                ggggAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGt                                                  <  2:2478850/101‑1 (MQ=255)
                                                   gAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTg                                               >  1:2479245/1‑101 (MQ=255)
                                                    aGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCGGCCCGGCGGTg                                                      >  2:2479247/1‑93 (MQ=255)
                                                    aGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGccc                                                             <  2:2478716/86‑1 (MQ=255)
                                                     gTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGc                                             <  1:2478613/101‑1 (MQ=255)
                                                        gtgCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATc                                          <  1:2478449/101‑1 (MQ=255)
                                                        gtgCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGACGGTGATGTTTGGCATc                                          >  1:2479250/1‑101 (MQ=255)
                                                         tgCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCg                                                                                            <  2:2478204/50‑1 (MQ=255)
                                                          gcgcCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATcac                                        <  2:2479169/101‑1 (MQ=255)
                                                             ccGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGc                                     <  2:2478842/101‑1 (MQ=255)
                                                              cGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCa                                    <  2:2478604/101‑1 (MQ=255)
                                                              cGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCa                                    >  1:2479255/1‑101 (MQ=255)
                                                              cGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCa                                    >  2:2479254/1‑101 (MQ=255)
                                                                agaTCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCaaa                                  <  2:2478758/101‑1 (MQ=255)
                                                                agaTCGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCaaa                                  <  1:2478706/101‑1 (MQ=255)
                                                                    cGAGAAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCag                              >  2:2479260/1‑101 (MQ=255)
                                                                     gagaAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCaga                             <  1:2479006/101‑1 (MQ=255)
                                                                      agaAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCagag                            <  1:2478857/101‑1 (MQ=255)
                                                                      agaAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCagag                            >  1:2479262/1‑101 (MQ=255)
                                                                       gaAAGCCCAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAgaga                           <  2:2478559/101‑1 (MQ=255)
                                                                            cccAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTg                                                      <  1:2478535/69‑1 (MQ=255)
                                                                            cccAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATAtt                      >  1:2479267/1‑101 (MQ=255)
                                                                            cccAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATAtt                      >  2:2479266/1‑101 (MQ=255)
                                                                              cAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCa                    <  2:2478813/101‑1 (MQ=255)
                                                                              cAGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCa                    <  2:2478656/101‑1 (MQ=255)
                                                                               aGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAg                   <  2:2478950/101‑1 (MQ=255)
                                                                               aGCGCGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAg                   <  1:2478923/101‑1 (MQ=255)
                                                                                gcgcGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGc                  <  2:2478784/101‑1 (MQ=255)
                                                                                gcgcGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGc                  <  1:2478651/101‑1 (MQ=255)
                                                                                gcgcGGCGATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGc                  >  2:2479271/1‑101 (MQ=255)
                                                                                  gcggcgATATCGAGAACTGCCATCGACAGTGCGCGGGTg                                                                              <  1:2478877/39‑1 (MQ=255)
                                                                                  gcggcgATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCg                 <  1:2478816/100‑1 (MQ=255)
                                                                                  gcggcgATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGt                <  2:2478873/101‑1 (MQ=255)
                                                                                  gcggcgATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGt                <  2:2478701/101‑1 (MQ=255)
                                                                                   cggcgATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAg                   >  1:2479277/1‑97 (MQ=255)
                                                                                   cggcgATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt               <  2:2478800/101‑1 (MQ=255)
                                                                                   cggcgATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt               <  2:2478738/101‑1 (MQ=255)
                                                                                   cggcgATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGtt               >  2:2479275/1‑101 (MQ=255)
                                                                                    ggcgATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTg              >  2:2479279/1‑101 (MQ=255)
                                                                                      cgATATCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGAt            >  2:2479282/1‑101 (MQ=255)
                                                                                        atatCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATgc          <  1:2478787/101‑1 (MQ=255)
                                                                                         tatCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATgcg         >  1:2479288/1‑101 (MQ=255)
                                                                                         tatCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATgcg         >  2:2479287/1‑101 (MQ=255)
                                                                                         tatCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATgcg         <  1:2478579/101‑1 (MQ=255)
                                                                                          atCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATgcgc        <  2:2478673/101‑1 (MQ=255)
                                                                                          atCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATgcgc        <  1:2479087/101‑1 (MQ=255)
                                                                                           tCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGcc       >  1:2479292/1‑101 (MQ=255)
                                                                                           tCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGcc       <  2:2479031/101‑1 (MQ=255)
                                                                                           tCGAGAACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGcc       <  1:2478756/101‑1 (MQ=255)
                                                                                             gagaACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCAt     <  1:2478727/101‑1 (MQ=255)
                                                                                               gaACTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATcc   >  2:2479295/1‑101 (MQ=255)
                                                                                                  |                                                                                                  
ATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCA  >  NC_000913/1353739‑1353935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑