BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	132,677	A→G	T355A (ACC→GCC)	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	132,677	0	A	G	100.0%	1154.8 / NA	279	T355A (ACC→GCC)	acnB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (130/149); total (130/149)

Only 100 of 287 total aligned reads displayed.

GCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCACCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCTGGCGCAAAA  >  NC_000913/132579‑132774

 cGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGAccgcca                                                                                                >  2:250460/1‑101 (MQ=255)

   aaCTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCaaa                                                                                              >  1:250464/1‑101 (MQ=255)

       gctgGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAgcgc                                                                                          >  1:250468/1‑101 (MQ=255)

        ctgGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGcgcg                                                                                         >  2:250469/1‑101 (MQ=255)

        ctgGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGcgcg                                                                                         <  1:250011/101‑1 (MQ=255)

         tgGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGt                                                                                        <  1:250024/101‑1 (MQ=255)

          ggCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTg                                                                                       <  2:250090/101‑1 (MQ=255)

           gCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGa                                                                                      >  1:250472/1‑101 (MQ=255)

                    gAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTgg                                                                             >  2:250479/1‑101 (MQ=255)

                     aaCTGAAAACCGACGTGCTGATTGATGAAGCGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGt                                                                            >  2:250482/1‑101 (MQ=255)

                      aCTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTc                                                                           >  1:250484/1‑101 (MQ=255)

                       cTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCt                                                                          <  1:250098/101‑1 (MQ=255)

                        tGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTg                                                                         <  2:250164/101‑1 (MQ=255)

                        tGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTg                                                                         >  1:250488/1‑101 (MQ=255)

                        tGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTg                                                                         >  2:250487/1‑101 (MQ=255)

                           aaaCCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCg                                                                      <  1:249999/101‑1 (MQ=255)

                           aaaCCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCg                                                                      >  1:250492/1‑101 (MQ=255)

                            aaCCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGc                                                                     <  1:249696/101‑1 (MQ=255)

                              ccGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGcac                                                                   <  1:250045/101‑1 (MQ=255)

                               cGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGcaca                                                                  >  1:250496/1‑101 (MQ=255)

                                gACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAg                                                                 >  1:250499/1‑101 (MQ=255)

                                gACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAg                                                                 <  1:249917/101‑1 (MQ=255)

                                 aCGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGt                                                                <  2:249880/101‑1 (MQ=255)

                                  cGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTg                                                               >  2:250500/1‑101 (MQ=255)

                                   gTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGca                                                                    <  2:250048/95‑1 (MQ=255)

                                   gTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGa                                                              >  1:250505/1‑101 (MQ=255)

                                    tGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCg                                                                      <  1:250071/92‑1 (MQ=255)

                                    tGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGAt                                                             <  1:249956/101‑1 (MQ=255)

                                       tgattgatGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGAtgtg                                                          >  1:250512/1‑101 (MQ=255)

                                       tgattgatGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGAtgtg                                                          <  1:250203/101‑1 (MQ=255)

                                       tgattgatGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGAtgtg                                                          >  2:250509/1‑101 (MQ=255)

                                       tgattgatGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGAtgtg                                                          >  2:250511/1‑101 (MQ=255)

                                       tgattgatGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGAtgtg                                                          <  2:250175/101‑1 (MQ=255)

                                         attgatGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGtt                                                        <  1:250085/101‑1 (MQ=255)

                                           tgatgaAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTcc                                                      <  2:250133/101‑1 (MQ=255)

                                           tgatgaAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTcc                                                      >  2:250516/1‑101 (MQ=255)

                                           tgatgaAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTcc                                                      <  1:249982/101‑1 (MQ=255)

                                             atgaAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGt                                                    >  1:250518/1‑101 (MQ=255)

                                             atgaAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGt                                                    <  2:250187/101‑1 (MQ=255)

                                             atgaAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGt                                                    <  2:250044/101‑1 (MQ=255)

                                               gaAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCa                                                  >  2:250523/1‑101 (MQ=255)

                                                aaGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAg                                                 >  1:250526/1‑101 (MQ=255)

                                                 aGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAgg                                                >  2:250528/1‑101 (MQ=255)

                                                  gtgcgtgcTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGc                                               <  1:249892/101‑1 (MQ=255)

                                                   tgcgtgcTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCg                                              <  2:250243/101‑1 (MQ=255)

                                                   tgcgtgcTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCg                                              <  2:250258/101‑1 (MQ=255)

                                                     cgtgcTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGaa                                            <  1:250160/101‑1 (MQ=255)

                                                     cgtgcTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGaa                                            <  2:250151/101‑1 (MQ=255)

                                                      gtgcTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGaaa                                           >  1:250534/1‑101 (MQ=255)

                                                      gtgcTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGaaa                                           >  1:250531/1‑101 (MQ=255)

                                                       tgcTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAg                                          >  2:250536/1‑101 (MQ=255)

                                                       tgcTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAg                                          <  1:250222/101‑1 (MQ=255)

                                                          tggtggCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATg                                       >  2:250539/1‑101 (MQ=255)

                                                            gtggCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTc                                     <  2:249955/101‑1 (MQ=255)

                                                             tggCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCg                                    >  2:250545/1‑101 (MQ=255)

                                                             tggCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCg                                    >  1:250543/1‑101 (MQ=255)

                                                              ggCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGc                                   <  2:249926/101‑1 (MQ=255)

                                                               gCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCt                                  >  1:250547/1‑101 (MQ=255)

                                                                  gTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTgag                               <  1:250259/101‑1 (MQ=255)

                                                                  gTATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTgag                               >  1:250551/1‑101 (MQ=255)

                                                                   tATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTgaga                              <  2:250284/101‑1 (MQ=255)

                                                                   tATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTgaga                              <  1:249831/101‑1 (MQ=255)

                                                                   tATTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTgaga                              >  1:250555/1‑101 (MQ=255)

                                                                    aTTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGagag                             <  1:250143/101‑1 (MQ=255)

                                                                    aTTCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGagag                             >  1:250558/1‑101 (MQ=255)

                                                                     ttCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGc                            <  2:250100/101‑1 (MQ=255)

                                                                     ttCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGc                            <  2:250075/101‑1 (MQ=255)

                                                                     ttCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGc                            <  2:250001/101‑1 (MQ=255)

                                                                     ttCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGc                            >  2:250559/1‑101 (MQ=255)

                                                                     ttCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGc                            <  1:250184/101‑1 (MQ=255)

                                                                     ttCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGc                            <  2:250224/101‑1 (MQ=255)

                                                                      tCCGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCg                           <  2:250105/101‑1 (MQ=255)

                                                                       ccGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGa                          <  1:250146/101‑1 (MQ=255)

                                                                        cGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCg                           <  2:250314/99‑1 (MQ=255)

                                                                        cGCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGAt                         >  2:250562/1‑101 (MQ=255)

                                                                         gCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATc                        <  1:250241/101‑1 (MQ=255)

                                                                         gCTGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATc                        <  2:250212/101‑1 (MQ=255)

                                                                           tGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGaaa                                           >  1:250571/1‑80 (MQ=255)

                                                                           tGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATcgc                      >  1:250566/1‑101 (MQ=255)

                                                                           tGATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATcgc                      >  2:250568/1‑101 (MQ=255)

                                                                            gATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATcgcg                     <  1:250169/101‑1 (MQ=255)

                                                                            gATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATcgcg                     <  2:250350/101‑1 (MQ=255)

                                                                            gATTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATcgcg                     <  1:250294/101‑1 (MQ=255)

                                                                             aTTATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCgg                    >  2:250572/1‑101 (MQ=255)

                                                                               tATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCt                  <  1:250137/101‑1 (MQ=255)

                                                                               tATCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCCGAGAGCGATCGCGGCt                  >  2:250576/1‑101 (MQ=255)

                                                                                aTCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCtt                 <  2:250391/101‑1 (MQ=255)

                                                                                aTCGGGCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCtt                 <  1:250110/101‑1 (MQ=255)

                                                                                   gggCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTtctc              <  2:250268/101‑1 (MQ=255)

                                                                                    ggCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATcgc                      >  2:250580/1‑92 (MQ=255)

                                                                                     gCGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGc            <  1:250355/101‑1 (MQ=255)

                                                                                      cGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCt           <  2:250201/101‑1 (MQ=255)

                                                                                      cGTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCt           <  1:250423/101‑1 (MQ=255)

                                                                                       gTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCTg          >  2:250584/1‑101 (MQ=255)

                                                                                       gTGGCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCTg          >  1:250583/1‑101 (MQ=255)

                                                                                         ggCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCTGgc        >  1:250588/1‑101 (MQ=255)

                                                                                          gCCTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCTGgcg       <  2:249937/101‑1 (MQ=255)

                                                                                           ccTGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATc                        >  2:250591/1‑83 (MQ=255)

                                                                                             tGACCGCCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCTGGCGCaa    >  1:250593/1‑101 (MQ=255)

                                                                                               accgccAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCTGGCGCaaaa  >  1:250598/1‑101 (MQ=255)

GCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCGCTGATTATCGGGCGTGGCCTGACCACCAAAGCGCGTGAAGCACTTGGTCTGCCGCACAGTGATGTGTTCCGTCAGGCGAAAGATGTCGCTGAGAGCGATCGCGGCTTCTCGCTGGCGCAAAA  >  NC_000913/132579‑132774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`