Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,643,313 T→C N324S (AAC→AGC)  rlmN ← 23S rRNA m(2)A2503 methyltransferase/tRNA m(2)A37 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,643,3130TC99.7% 1525.8 / ‑5.1 370N324S (AAC→AGC) rlmN23S rRNA m(2)A2503 methyltransferase/tRNA m(2)A37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (159/210);  minor base A (0/1);  total (159/211)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 372 total aligned reads displayed.

GACCACAGGCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGTTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTGGTGCGC  >  NC_000913/2643215‑2643410
                                                                                                  |                                                                                                 
gACCACAGGCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTc                                                                                                 <  2:4763396/101‑1 (MQ=255)
gACCACAGGCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTc                                                                                                 <  2:4763268/101‑1 (MQ=255)
gACCACAGGCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTc                                                                                                 <  2:4763363/101‑1 (MQ=255)
 aCCACAGGCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCg                                                                                                >  2:4763911/1‑101 (MQ=255)
 aCCACAGGCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCg                                                                                                <  2:4763682/101‑1 (MQ=255)
 aCCACAGGCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCg                                                                                                <  2:4763046/101‑1 (MQ=255)
     caGGCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCt                                                                                            <  1:4763474/101‑1 (MQ=255)
      aGGCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTg                                                                                           >  1:4763915/1‑101 (MQ=255)
       ggCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGc                                                                                          <  1:4763426/101‑1 (MQ=255)
         cagcaTCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGt                                                                                        >  2:4763919/1‑101 (MQ=255)
           gcaTCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTcc                                                                                      <  2:4763183/101‑1 (MQ=255)
           gcaTCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTcc                                                                                      <  1:4763379/101‑1 (MQ=255)
            caTCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCa                                                                                     >  1:4763927/1‑101 (MQ=255)
            caTCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCa                                                                                     >  2:4763926/1‑101 (MQ=255)
             aTCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCAt                                                                                    <  2:4763340/101‑1 (MQ=255)
              tCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATa                                                                                   >  1:4763935/1‑101 (MQ=255)
               cGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATa                                                                                   <  2:4763435/100‑1 (MQ=255)
                gATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACg                                                                                 <  2:4763825/101‑1 (MQ=255)
                gATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACg                                                                                 >  1:4763939/1‑101 (MQ=255)
                 atatCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACgg                                                                                >  2:4763941/1‑101 (MQ=255)
                  tatCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGgc                                                                               <  2:4763758/101‑1 (MQ=255)
                  tatCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGgc                                                                               >  1:4763944/1‑101 (MQ=255)
                      atcaCCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCAc                                                                           <  2:4763233/101‑1 (MQ=255)
                        caccacGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCAccc                                                                         >  2:4763950/1‑101 (MQ=255)
                        caccacGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCAccc                                                                         >  1:4763951/1‑101 (MQ=255)
                            acGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGa                                                                     >  2:4763955/1‑101 (MQ=255)
                            acGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGa                                                                     <  2:4763166/101‑1 (MQ=255)
                            acGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGa                                                                     <  2:4763216/101‑1 (MQ=255)
                               aGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACg                                                                  >  2:4763963/1‑101 (MQ=255)
                               aGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACg                                                                  >  1:4763962/1‑101 (MQ=255)
                                gTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACgg                                                                 <  2:4763300/101‑1 (MQ=255)
                                 ttttACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACggg                                                                >  2:4763970/1‑101 (MQ=255)
                                  tttACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGt                                                               >  1:4763972/1‑101 (MQ=255)
                                   ttACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGtt                                                              <  1:4763256/101‑1 (MQ=255)
                                   ttACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGtt                                                              <  1:4763175/101‑1 (MQ=255)
                                   ttACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTACTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGtt                                                              >  2:4763975/1‑101 (MQ=255)
                                    tACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTc                                                             >  1:4763977/1‑101 (MQ=255)
                                       gCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCAc                                                          <  1:4763073/101‑1 (MQ=255)
                                         acaATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACgg                                                        >  2:4763983/1‑101 (MQ=255)
                                          caATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACggg                                                       <  2:4763250/101‑1 (MQ=255)
                                           aaTAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACg                                                         >  2:4763989/1‑98 (MQ=255)
                                           aaTAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGa                                                      <  2:4763527/101‑1 (MQ=255)
                                            aTAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGAt                                                     <  1:4763234/101‑1 (MQ=255)
                                               gtggtgAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAg                                                  >  1:4763992/1‑101 (MQ=255)
                                                 ggtgAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTcc                                                            >  2:4763996/1‑89 (MQ=255)
                                                   tgAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTg                                              <  2:4763423/101‑1 (MQ=255)
                                                    gAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGa                                             <  1:4763540/101‑1 (MQ=255)
                                                    gAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGa                                             >  1:4764001/1‑101 (MQ=255)
                                                    gAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGa                                             <  2:4763120/101‑1 (MQ=255)
                                                     aaaCCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCGCCCGGGAACGGGTTCCACGGGATCAGGTTGAt                                            <  1:4763350/101‑1 (MQ=255)
                                                     aaaCCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGAt                                            >  2:4764004/1‑101 (MQ=255)
                                                     aaaCCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGAt                                            >  1:4764005/1‑101 (MQ=255)
                                                       aCCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCt                                          <  1:4763008/101‑1 (MQ=255)
                                                       aCCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCt                                          <  2:4763348/101‑1 (MQ=255)
                                                       aCCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGGTGATCt                                          >  2:4764012/1‑101 (MQ=255)
                                                         cGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTa                                        <  2:4763309/101‑1 (MQ=255)
                                                          gTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTg                                              >  2:4764018/1‑94 (MQ=255)
                                                           tAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTaca                                      >  2:4764021/1‑101 (MQ=255)
                                                            aGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTacac                                     >  1:4764025/1‑101 (MQ=255)
                                                              cTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACgg                                   <  2:4763472/101‑1 (MQ=255)
                                                              cTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACgg                                   <  2:4763614/101‑1 (MQ=255)
                                                              cTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACgg                                   <  1:4763271/101‑1 (MQ=255)
                                                              cTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACgg                                   >  1:4764029/1‑101 (MQ=255)
                                                                catcaGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCg                                 <  1:4763761/101‑1 (MQ=255)
                                                                catcaGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCg                                 <  1:4763826/101‑1 (MQ=255)
                                                                catcaGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCg                                 <  1:4763596/101‑1 (MQ=255)
                                                                   caGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTAt                              <  1:4763440/101‑1 (MQ=255)
                                                                    aGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATc                             >  1:4764035/1‑101 (MQ=255)
                                                                    aGCACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATc                             >  2:4764032/1‑101 (MQ=255)
                                                                      cACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCtt                           <  2:4763410/101‑1 (MQ=255)
                                                                      cACTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCtt                           >  1:4764039/1‑101 (MQ=255)
                                                                       aCTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCttt                          >  2:4764041/1‑101 (MQ=255)
                                                                        cTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTc                         <  1:4763667/101‑1 (MQ=255)
                                                                        cTTTTGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTc                         >  2:4764046/1‑101 (MQ=255)
                                                                           ttGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTcagc                      <  1:4763389/101‑1 (MQ=255)
                                                                            tGAGAAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTcagca                     >  1:4764051/1‑101 (MQ=255)
                                                                               gaAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCg                                 >  2:4764054/1‑86 (MQ=255)
                                                                               gaAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGtt                  <  2:4763739/101‑1 (MQ=255)
                                                                               gaAACGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGtt                  <  1:4763524/101‑1 (MQ=255)
                                                                                aaaCGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTc                 >  1:4764056/1‑101 (MQ=255)
                                                                                 aaCGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTcc                <  2:4763588/101‑1 (MQ=255)
                                                                                 aaCGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTcc                <  2:4763499/101‑1 (MQ=255)
                                                                                 aaCGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTcc                <  2:4763483/101‑1 (MQ=255)
                                                                                 aaCGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTcc                <  1:4763683/101‑1 (MQ=255)
                                                                                 aaCGGTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTcc                >  1:4764060/1‑101 (MQ=255)
                                                                                    ggTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGcc             <  1:4763319/101‑1 (MQ=255)
                                                                                    ggTCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGcc             >  2:4764064/1‑101 (MQ=255)
                                                                                    ggTCGATACGGCTGATCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGcc             <  1:4763488/101‑1 (MQ=255)
                                                                                      tCGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGtt                                               <  2:4763205/65‑1 (MQ=255)
                                                                                       cGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGt          >  1:4764067/1‑101 (MQ=255)
                                                                                       cGATACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGt          <  1:4763203/101‑1 (MQ=255)
                                                                                         aTACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTg        <  2:4763628/101‑1 (MQ=255)
                                                                                         aTACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTg        <  1:4763502/101‑1 (MQ=255)
                                                                                         aTACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTg        <  1:4763326/101‑1 (MQ=255)
                                                                                          tACGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTgg       <  2:4763404/101‑1 (MQ=255)
                                                                                           aCGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTGGt      <  1:4763705/101‑1 (MQ=255)
                                                                                            cGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTGGTg     <  2:4763453/101‑1 (MQ=255)
                                                                                            cGGCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTGGTg     <  2:4763332/101‑1 (MQ=255)
                                                                                             ggCTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTGGTgc    >  2:4764074/1‑101 (MQ=255)
                                                                                               cTGCTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTGGTgcgc  >  1:4764078/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GACCACAGGCAGCATCGATATCATCACCACGAGTTTTACGCACAATAGTGGTGAAACCGTAGCTCATCAGCACTTTTGAGAAACGGTCGATACGGCTGTTCGAGCTGCGTCCATACGGCGCACCCGGGAACGGGTTCCACGGGATCAGGTTGATCTTACACGGCGTATCTTTCAGCAGTTCCGCCAGTTGGTGCGC  >  NC_000913/2643215‑2643410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑