BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,219	(C)_6→7	coding (200/672 nt)	queE ←	putative 7‑carboxy‑7‑deazaguanine synthase QueE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,213	1	.	C	100.0%	1141.0 / NA	270	A69G (GCT→GGT)	queE	putative 7‑carboxy‑7‑deazaguanine synthase QueE
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (156/114); total (156/114)

Only 100 of 312 total aligned reads displayed.

CAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCAC  >  NC_000913/2905113‑2905308

 agcaAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑c                                                                                                >  1:5386773/1‑101 (MQ=255)

   caAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccc                                                                                              <  1:5386119/101‑1 (MQ=255)

    aaaTCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑cccc                                                                                             <  1:5386346/101‑1 (MQ=255)

    aaaTCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑cccc                                                                                             <  1:5386435/101‑1 (MQ=255)

     aaTCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccccc                                                                                            >  1:5386777/1‑101 (MQ=255)

       tCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccccccc                                                                                          >  2:5386781/1‑100 (MQ=255)

        cATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccccccca                                                                                         >  2:5386786/1‑99 (MQ=255)

        cATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑ccccccca                                                                                         >  1:5386785/1‑99 (MQ=255)

         aTGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑cccccccac                                                                                        >  1:5386790/1‑98 (MQ=255)

          tGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑cccccccact                                                                                       <  2:5386513/101‑5 (MQ=255)

          tGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑cccccccact                                                                                       <  2:5386038/101‑5 (MQ=255)

          tGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑cccccccact                                                                                       >  1:5386794/1‑97 (MQ=255)

           gAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑cccccccactt                                                                                      <  2:5386261/101‑6 (MQ=255)

           gAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑cccccccactt                                                                                      <  1:5386293/101‑6 (MQ=255)

             aTGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTAt                                                                                     >  2:5386799/1‑101 (MQ=255)

              tGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATc                                                                                    >  1:5386802/1‑101 (MQ=255)

                cAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAc                                                                                  >  1:5386805/1‑101 (MQ=255)

                 aaGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAct                                                                                 >  2:5386808/1‑101 (MQ=255)

                 aaGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCAct                                                                                 <  2:5386317/101‑1 (MQ=255)

                    gCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCActctc                                                                              <  1:5386136/101‑1 (MQ=255)

                    gCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCActctc                                                                              >  1:5386813/1‑101 (MQ=255)

                    gCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCActctc                                                                              <  2:5386189/101‑1 (MQ=255)

                     cTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCActctct                                                                             >  2:5386814/1‑101 (MQ=255)

                       caccacCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCttt                                                                           >  2:5386819/1‑101 (MQ=255)

                        accaccCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTg                                                                          >  2:5386822/1‑101 (MQ=255)

                          caccCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGt                                                                        >  2:5386825/1‑101 (MQ=255)

                          caccCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGt                                                                        >  1:5386826/1‑101 (MQ=255)

                           accCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTc                                                                       >  1:5386830/1‑101 (MQ=255)

                            cccGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTc                                                                       <  1:5386809/100‑1 (MQ=255)

                             ccGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCtt                                                                     <  2:5386359/101‑1 (MQ=255)

                             ccGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCtt                                                                     <  1:5386330/101‑1 (MQ=255)

                             ccGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCtt                                                                     <  1:5386443/101‑1 (MQ=255)

                              cGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTc                                                                    >  2:5386833/1‑101 (MQ=255)

                               gTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCg                                                                   >  1:5386836/1‑101 (MQ=255)

                                 aaTCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGcc                                                                 >  2:5386839/1‑101 (MQ=255)

                                  aTCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCa                                                                >  1:5386841/1‑101 (MQ=255)

                                   tCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAg                                                               <  2:5386243/101‑1 (MQ=255)

                                     accacATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGaa                                                             >  2:5386845/1‑101 (MQ=255)

                                      ccacATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAAt                                                            <  1:5386514/101‑1 (MQ=255)

                                       cacaTGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATg                                                           >  2:5386849/1‑101 (MQ=255)

                                        acaTGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGc                                                          >  1:5386851/1‑101 (MQ=255)

                                          aTGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTg                                                        >  1:5386856/1‑101 (MQ=255)

                                            gCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGaa                                                      <  2:5386403/101‑1 (MQ=255)

                                            gCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGaa                                                      >  2:5386858/1‑101 (MQ=255)

                                            gCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGaa                                                      <  2:5386434/101‑1 (MQ=255)

                                            gCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGaa                                                      <  1:5386478/101‑1 (MQ=255)

                                            gCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGaa                                                      <  2:5386167/101‑1 (MQ=255)

                                              cgcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGaaaa                                                    >  1:5386861/1‑101 (MQ=255)

                                              cgcgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACCCTCTTTGGTCTTCGCCAGaa                                                             <  2:5386354/92‑1 (MQ=255)

                                                cgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAgg                                                  <  2:5386366/101‑1 (MQ=255)

                                                cgcgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAgg                                                  >  2:5386866/1‑101 (MQ=255)

                                                  cgGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGga                                                >  1:5386868/1‑101 (MQ=255)

                                                       tATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCtt                                                                     <  2:5386389/75‑1 (MQ=255)

                                                       tATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACtt                                           <  1:5386376/101‑1 (MQ=255)

                                                        aTCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTc                                          >  1:5386872/1‑101 (MQ=255)

                                                          cccTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTccc                                        >  2:5386877/1‑101 (MQ=255)

                                                            cTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGa                                      <  2:5386494/101‑1 (MQ=255)

                                                            cTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGa                                      >  2:5386880/1‑101 (MQ=255)

                                                             tGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGAt                                     <  1:5386025/101‑1 (MQ=255)

                                                              ggCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATc                                    >  1:5386882/1‑101 (MQ=255)

                                                              ggCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATc                                    <  2:5386472/101‑1 (MQ=255)

                                                                 gACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTc                                 >  1:5386886/1‑101 (MQ=255)

                                                                 gACCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTc                                 >  2:5386887/1‑101 (MQ=255)

                                                                  aCCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCa                                >  1:5386891/1‑101 (MQ=255)

                                                                  aCCAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCa                                <  1:5386554/101‑1 (MQ=255)

                                                                    cAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAg                              >  2:5386895/1‑101 (MQ=255)

                                                                    cAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAg                              >  1:5386896/1‑101 (MQ=255)

                                                                    cAATGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAg                              <  1:5386358/101‑1 (MQ=255)

                                                                      aTGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCt                            >  2:5386902/1‑101 (MQ=255)

                                                                      aTGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCt                            <  1:5386408/101‑1 (MQ=255)

                                                                       tGACAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCtt                           >  1:5386904/1‑101 (MQ=255)

                                                                         aCAGCCAGCAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCtttt                         <  1:5386066/101‑1 (MQ=255)

                                                                          cagccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTc                        <  2:5386579/101‑1 (MQ=255)

                                                                          cagccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTc                        <  2:5386132/101‑1 (MQ=255)

                                                                          cagccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTc                        >  2:5386911/1‑101 (MQ=255)

                                                                          cagccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTc                        >  1:5386908/1‑101 (MQ=255)

                                                                            gccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTccc                      >  2:5386916/1‑101 (MQ=255)

                                                                            gccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTccc                      >  1:5386915/1‑101 (MQ=255)

                                                                             ccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCa                     <  1:5386396/101‑1 (MQ=255)

                                                                             ccagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCa                     <  2:5386874/101‑1 (MQ=255)

                                                                              cagcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAg                    >  1:5386921/1‑101 (MQ=255)

                                                                               agcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAgg                   <  1:5386169/101‑1 (MQ=255)

                                                                                gcAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgt                  <  2:5386297/101‑1 (MQ=255)

                                                                                 cAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAGTGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtg                 >  1:5386924/1‑101 (MQ=255)

                                                                                 cAAATCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtg                 <  2:5386451/101‑1 (MQ=255)

                                                                                  aaaTCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtgt                <  1:5386265/101‑1 (MQ=255)

                                                                                  aaaTCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtgt                >  2:5386925/1‑101 (MQ=255)

                                                                                  aaaTCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtgt                >  1:5386930/1‑101 (MQ=255)

                                                                                   aaTCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGgtgtg               >  2:5386931/1‑101 (MQ=255)

                                                                                     tCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGtt             >  2:5386937/1‑101 (MQ=255)

                                                                                     tCTTCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGtt             >  1:5386936/1‑101 (MQ=255)

                                                                                        tCACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTg           >  1:5386942/1‑100 (MQ=255)

                                                                                         cACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgt         <  2:5386209/101‑1 (MQ=255)

                                                                                         cACTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgt         <  1:5386278/101‑1 (MQ=255)

                                                                                          aCTGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGgtg        >  2:5386944/1‑101 (MQ=255)

                                                                                           cTGCTCGCAGCCCCCCCACTTATCCCTCTCTCTGGTCTTCGcc                                                                 <  2:5386307/43‑1 (MQ=255)

                                                                                            tGCTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTc      <  1:5386222/101‑1 (MQ=255)

                                                                                              cTCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGc    >  2:5386948/1‑101 (MQ=255)

                                                                                               tCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCa   <  2:5386653/101‑1 (MQ=255)

                                                                                               tCGCAGCCCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCa   >  1:5386951/1‑101 (MQ=255)

CAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCTGGCGACCAATGACAGCCAGCAAATCTTCACTGCTCGCAG‑CCCCCCACTTATCACTCTCTTTGGTCTTCGCCAGAATGCTGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCAC  >  NC_000913/2905113‑2905308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`