BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,218,064	C→T	S319L (TCG→TTG)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,218,064	0	C	T	100.0%	1522.1 / NA	352	S319L (TCG→TTG)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (186/166); total (186/166)

Only 100 of 352 total aligned reads displayed.

CTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTCGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGTTCATCACC  >  NC_000913/4217966‑4218161

  ggtGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCcg                                                                                               <  1:7829110/101‑1 (MQ=255)

   gtgtGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCcgt                                                                                              <  2:7829459/101‑1 (MQ=255)

   gtgtGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCcgt                                                                                              <  1:7828905/101‑1 (MQ=255)

    tgtgAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCcgtc                                                                                             <  2:7829274/101‑1 (MQ=255)

    tgtgAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCcgtc                                                                                             >  1:7829806/1‑101 (MQ=255)

     gtgAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCcgtcg                                                                                            <  1:7829422/101‑1 (MQ=255)

      tgAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCcgtcgt                                                                                           >  2:7829811/1‑101 (MQ=255)

         aaCCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCa                                                                                        <  1:7829153/101‑1 (MQ=255)

         aaCCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCa                                                                                        >  1:7829814/1‑101 (MQ=255)

          aCCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTcga                                                                                       >  2:7829815/1‑99 (MQ=255)

          aCCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCaa                                                                                       <  2:7829019/101‑1 (MQ=255)

             tCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTg                                                                                    <  2:7829216/101‑1 (MQ=255)

             tCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTg                                                                                    <  2:7829377/101‑1 (MQ=255)

             tCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTg                                                                                    <  1:7829310/101‑1 (MQ=255)

               cACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGc                                                                                  >  1:7829822/1‑101 (MQ=255)

                  gCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGa                                                                               >  2:7829825/1‑101 (MQ=255)

                  gCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGa                                                                               >  1:7829826/1‑101 (MQ=255)

                    cGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGaaa                                                                             <  1:7829244/101‑1 (MQ=255)

                    cGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGaaa                                                                             >  2:7829830/1‑101 (MQ=255)

                     ggATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGaaaa                                                                            <  1:7829289/101‑1 (MQ=255)

                      gATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGaaaaa                                                                           >  1:7829835/1‑101 (MQ=255)

                      gATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGaaaaa                                                                           <  1:7829172/101‑1 (MQ=255)

                       aTCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAAc                                                                          >  2:7829839/1‑101 (MQ=255)

                       aTCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAAc                                                                          <  2:7829145/101‑1 (MQ=255)

                           ggAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTcg                                                                      >  2:7829844/1‑101 (MQ=255)

                           ggAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTcg                                                                      <  2:7829191/101‑1 (MQ=255)

                              aCTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTcgacg                                                                   >  2:7829848/1‑101 (MQ=255)

                              aCTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTcgacg                                                                   <  1:7829397/101‑1 (MQ=255)

                                tGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCgacgac                                                                 <  1:7829363/101‑1 (MQ=255)

                                  gcgcGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACaa                                                               >  2:7829852/1‑101 (MQ=255)

                                    gcgTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACaaaa                                                             >  1:7829854/1‑101 (MQ=255)

                                      gTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACt                                                           <  1:7829592/101‑1 (MQ=255)

                                       tCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTa                                                          <  2:7829424/101‑1 (MQ=255)

                                       tCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTa                                                          >  1:7829858/1‑101 (MQ=255)

                                          cTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACaaa                                                              >  1:7829864/1‑94 (MQ=255)

                                           tttGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGc                                                      <  1:7829571/101‑1 (MQ=255)

                                           tttGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGc                                                      >  2:7829866/1‑101 (MQ=255)

                                            ttGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGc                                                      <  2:7829322/100‑1 (MQ=255)

                                              gCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTAtt                                                        >  1:7829874/1‑96 (MQ=255)

                                              gCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAg                                                   >  1:7829870/1‑101 (MQ=255)

                                               cacaAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCcgtcg                                                                                            <  1:7829340/59‑1 (MQ=255)

                                               cacaAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGc                                                  <  1:7829453/101‑1 (MQ=255)

                                               cacaAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGc                                                  >  2:7829876/1‑101 (MQ=255)

                                                acaAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCt                                                 <  1:7829351/101‑1 (MQ=255)

                                                 caAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCtt                                                <  2:7829755/101‑1 (MQ=255)

                                                 caAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCtt                                                >  1:7829882/1‑101 (MQ=255)

                                                 caAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCtt                                                >  2:7829881/1‑101 (MQ=255)

                                                    gCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCca                                             <  1:7829500/101‑1 (MQ=255)

                                                    gCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCca                                             <  1:7829215/101‑1 (MQ=255)

                                                     cTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcag                                            <  2:7829173/101‑1 (MQ=255)

                                                     cTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATAGCCAGCTTCcag                                            >  1:7829888/1‑101 (MQ=255)

                                                      tATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagc                                           >  2:7829889/1‑101 (MQ=255)

                                                      tATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagc                                           <  1:7829440/101‑1 (MQ=255)

                                                      tATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagc                                           <  2:7829518/101‑1 (MQ=255)

                                                      tATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagc                                           <  1:7829511/101‑1 (MQ=255)

                                                       aTCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagca                                          >  1:7829894/1‑101 (MQ=255)

                                                       aTCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagca                                          <  1:7829315/101‑1 (MQ=255)

                                                         ccACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagcagc                                        <  1:7829832/101‑1 (MQ=255)

                                                          cACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagcagca                                       >  1:7829900/1‑101 (MQ=255)

                                                          cACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagcagca                                       <  2:7829446/101‑1 (MQ=255)

                                                          cACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagcagca                                       >  2:7829898/1‑101 (MQ=255)

                                                           aCGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagcagcag                                      >  1:7829903/1‑101 (MQ=255)

                                                           aCGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCcagcagcag                                      <  2:7829292/101‑1 (MQ=255)

                                                            cgcgAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCagcagcagc                                     >  2:7829907/1‑101 (MQ=255)

                                                              cgAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTg                                   >  2:7829912/1‑101 (MQ=255)

                                                               gAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGt                                  >  2:7829917/1‑101 (MQ=255)

                                                               gAAATATCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGt                                  >  1:7829916/1‑101 (MQ=255)

                                                                  atatCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCgg                               >  1:7829923/1‑101 (MQ=255)

                                                                   tatCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGa                              >  2:7829926/1‑101 (MQ=255)

                                                                    atCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGat                             <  2:7829229/101‑1 (MQ=255)

                                                                     tCCGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGata                            >  1:7829929/1‑101 (MQ=255)

                                                                       cGGGCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATg                          >  2:7829932/1‑101 (MQ=255)

                                                                         ggCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCgg                               <  2:7829306/94‑1 (MQ=255)

                                                                         ggCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATggg                        >  1:7829934/1‑101 (MQ=255)

                                                                          gCAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCt                                    <  2:7829251/88‑1 (MQ=255)

                                                                           cAAACTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCt                      <  1:7829530/101‑1 (MQ=255)

                                                                             aaCTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTAc                    >  2:7829940/1‑101 (MQ=255)

                                                                             aaCTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTAc                    <  1:7829272/101‑1 (MQ=255)

                                                                              aCTGCTGGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACa                   >  1:7829943/1‑101 (MQ=255)

                                                                                tgctgGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAg                 >  2:7829947/1‑101 (MQ=255)

                                                                                tgctgGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAg                 >  1:7829948/1‑101 (MQ=255)

                                                                                 gctgGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGt                >  1:7829954/1‑101 (MQ=255)

                                                                                  ctgGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGtt               >  2:7829958/1‑101 (MQ=255)

                                                                                  ctgGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGtt               <  1:7829471/101‑1 (MQ=255)

                                                                                  ctgGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGtt               >  1:7829959/1‑101 (MQ=255)

                                                                                   tgGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTc              >  2:7829963/1‑101 (MQ=255)

                                                                                   tgGCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTc              <  2:7829404/101‑1 (MQ=255)

                                                                                     gCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAGCCTCGACGACAAAACTAt                                                         <  1:7829710/56‑1 (MQ=255)

                                                                                     gCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCa            >  1:7829970/1‑101 (MQ=255)

                                                                                     gCTTATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCa            >  2:7829969/1‑101 (MQ=255)

                                                                                       ttATAACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGt          <  1:7829545/101‑1 (MQ=255)

                                                                                        tataACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGtt         >  2:7829976/1‑101 (MQ=255)

                                                                                        tataACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGtt         <  2:7829496/101‑1 (MQ=255)

                                                                                         ataACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGTtc        >  1:7829979/1‑101 (MQ=255)

                                                                                         ataACTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGTtc        <  2:7829528/101‑1 (MQ=255)

                                                                                           aaCTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGTtcat      >  1:7829983/1‑101 (MQ=255)

                                                                                            aCTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGTtcatc     <  2:7829553/101‑1 (MQ=255)

                                                                                             cTGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGTtcatca    >  2:7829986/1‑101 (MQ=255)

                                                                                              tGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGTTCATCAc   <  2:7829346/101‑1 (MQ=255)

                                                                                              tGCTTGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGTTCATCAc   >  1:7829989/1‑101 (MQ=255)

CTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCACAAGCTATCCACGCGAAATATCCGGGCAAACTGCTGGCTTATAACTGCTCGCCGTCGTTCAACTGGCAGAAAAACCTCGACGACAAAACTATTGCCAGCTTCCAGCAGCAGCTGTCGGATATGGGCTACAAGTTCCAGTTCATCACC  >  NC_000913/4217966‑4218161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`