BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	366,805	C→T	G236S (GGC→AGC)	lacI ←	DNA‑binding transcriptional repressor LacI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,805	0	C	T	100.0%	1364.5 / NA	316	G236S (GGC→AGC)	lacI	DNA‑binding transcriptional repressor LacI
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (147/169); total (147/169)

Only 100 of 321 total aligned reads displayed.

GATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATA  >  NC_000913/366707‑366901

 atatCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCtctc                                                                                               >  1:680063/1‑101 (MQ=255)

  tatCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCa                                                                                              >  2:680065/1‑101 (MQ=255)

  tatCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCa                                                                                              <  1:679464/101‑1 (MQ=255)

   atCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCAt                                                                                             <  2:679473/101‑1 (MQ=255)

   atCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCAt                                                                                             <  2:679560/101‑1 (MQ=255)

    tCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCAtt                                                                                            <  1:679490/101‑1 (MQ=255)

    tCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCAtt                                                                                            <  1:679449/101‑1 (MQ=255)

       gCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAg                                                                                         <  1:679860/101‑1 (MQ=255)

        cACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGc                                                                                        <  1:679633/101‑1 (MQ=255)

        cACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGc                                                                                        >  1:680070/1‑101 (MQ=255)

         aCCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCa                                                                                       >  1:680073/1‑101 (MQ=255)

          ccAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCAt                                                                                      <  2:679587/101‑1 (MQ=255)

           cAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCAtt                                                                                     <  2:679954/101‑1 (MQ=255)

           cAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCAtt                                                                                     >  1:680078/1‑101 (MQ=255)

           cAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCAtt                                                                                     >  2:680074/1‑101 (MQ=255)

            aaCGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCAttt                                                                                    >  2:680082/1‑101 (MQ=255)

             aCGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTg                                                                                   <  1:679341/101‑1 (MQ=255)

             aCGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTg                                                                                   <  1:679536/101‑1 (MQ=255)

               gcgcAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCa                                                                                 <  2:679213/101‑1 (MQ=255)

               gcgcAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCa                                                                                 >  2:680085/1‑101 (MQ=255)

                cgcAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCAt                                                                                >  1:680087/1‑101 (MQ=255)

                 gcAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATg                                                                               <  1:679050/101‑1 (MQ=255)

                 gcAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATg                                                                               >  1:680090/1‑101 (MQ=255)

                  cAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATgg                                                                              <  1:679729/101‑1 (MQ=255)

                     cccGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGttt                                                                           >  2:680094/1‑101 (MQ=255)

                     cccGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGttt                                                                           <  2:679686/101‑1 (MQ=255)

                     cccGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGttt                                                                           <  1:679989/101‑1 (MQ=255)

                       cGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTttgt                                                                         >  1:680097/1‑101 (MQ=255)

                        ggACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTttgtt                                                                        <  2:679628/101‑1 (MQ=255)

                        ggACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTttgtt                                                                        <  1:679664/101‑1 (MQ=255)

                         gACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTttgttg                                                                       >  1:680102/1‑101 (MQ=255)

                           cTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGaa                                                                     <  2:679461/101‑1 (MQ=255)

                            tCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGaaa                                                                    <  2:679773/101‑1 (MQ=255)

                            tCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGaaa                                                                    >  2:680105/1‑101 (MQ=255)

                            tCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGaaa                                                                    <  2:679397/101‑1 (MQ=255)

                             cGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGaaaa                                                                   <  1:679545/101‑1 (MQ=255)

                             cGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGaaaa                                                                   >  1:680107/1‑101 (MQ=255)

                              ggTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAAc                                                                  <  2:679717/101‑1 (MQ=255)

                              ggTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAAc                                                                  >  2:680109/1‑101 (MQ=255)

                                 aaTGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCgg                                                               >  2:680114/1‑101 (MQ=255)

                                 aaTGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCgg                                                               <  1:679516/101‑1 (MQ=255)

                                    ggCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACa                                                            <  1:679660/101‑1 (MQ=255)

                                     gcgcgcATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACAt                                                           <  1:679596/101‑1 (MQ=255)

                                      cgcgcATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATg                                                          >  1:680116/1‑101 (MQ=255)

                                       gcgcATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATgg                                                         <  2:679805/101‑1 (MQ=255)

                                         gcATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCa                                                       >  1:680120/1‑101 (MQ=255)

                                          cATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCAc                                                      <  2:679638/101‑1 (MQ=255)

                                          cATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCAc                                                      >  2:680122/1‑101 (MQ=255)

                                            ttGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTc                                                    <  2:679613/101‑1 (MQ=255)

                                                gcCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGt                                                <  1:679784/101‑1 (MQ=255)

                                                gcCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGt                                                >  1:680126/1‑101 (MQ=255)

                                                 cccAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTc                                               <  2:679733/101‑1 (MQ=255)

                                                 cccAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTc                                               <  1:679569/101‑1 (MQ=255)

                                                  ccAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCg                                              >  2:680130/1‑101 (MQ=255)

                                                  ccAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCg                                              <  1:679746/101‑1 (MQ=255)

                                                  ccAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCg                                              >  1:680131/1‑101 (MQ=255)

                                                   cAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGaaa                                                                    <  2:679575/78‑1 (MQ=255)

                                                    aGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGcc                                            >  1:680134/1‑101 (MQ=255)

                                                      cgcCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCtt                                          <  1:679719/101‑1 (MQ=255)

                                                       gcCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTc                                         >  1:680142/1‑101 (MQ=255)

                                                        ccATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTcc                                        >  2:680143/1‑101 (MQ=255)

                                                         cATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTccc                                       <  2:679665/101‑1 (MQ=255)

                                                          aTCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCg                                      >  1:680148/1‑101 (MQ=255)

                                                          aTCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCg                                      >  2:680149/1‑101 (MQ=255)

                                                            cTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGtt                                    <  1:679824/101‑1 (MQ=255)

                                                             tGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTc                                   >  1:680153/1‑101 (MQ=255)

                                                             tGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTc                                   >  2:680156/1‑101 (MQ=255)

                                                              gATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTcc                                  <  2:679436/101‑1 (MQ=255)

                                                              gATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTcc                                  <  2:679743/101‑1 (MQ=255)

                                                              gATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTcc                                  >  1:680159/1‑101 (MQ=255)

                                                               aTCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCg                                 <  1:679912/101‑1 (MQ=255)

                                                               aTCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCg                                 <  1:679703/101‑1 (MQ=255)

                                                                tCGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGc                                >  1:680163/1‑101 (MQ=255)

                                                                 cGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCt                               >  2:680166/1‑101 (MQ=255)

                                                                 cGTTGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCt                               <  2:679797/101‑1 (MQ=255)

                                                                   ttGGCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTAt                             >  1:680168/1‑101 (MQ=255)

                                                                      gCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCgg                          <  2:679634/101‑1 (MQ=255)

                                                                      gCAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCgg                          <  2:679692/101‑1 (MQ=255)

                                                                       cAACCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGc                         >  2:680172/1‑101 (MQ=255)

                                                                        aaCCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCt                        <  2:679530/101‑1 (MQ=255)

                                                                        aaCCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCt                        <  1:679694/101‑1 (MQ=255)

                                                                         aCCAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTg                       >  1:680177/1‑101 (MQ=255)

                                                                          ccAGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGa                      <  1:679764/101‑1 (MQ=255)

                                                                            aGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGagt                    >  1:680180/1‑99 (MQ=255)

                                                                            aGCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAAt                    >  2:680181/1‑101 (MQ=255)

                                                                             gCATCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAAtt                   >  1:680185/1‑101 (MQ=255)

                                                                               aTCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTg                 >  2:680190/1‑101 (MQ=255)

                                                                                tCGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGa                >  1:680191/1‑101 (MQ=255)

                                                                                 cGCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGAt               >  1:680194/1‑101 (MQ=255)

                                                                                  gCAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGAtt              >  2:680198/1‑101 (MQ=255)

                                                                                   cAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTg             >  1:680200/1‑101 (MQ=255)

                                                                                   cAGTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTg             <  2:679759/101‑1 (MQ=255)

                                                                                     gTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCg           <  2:679653/101‑1 (MQ=255)

                                                                                     gTGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCg           >  1:680203/1‑101 (MQ=255)

                                                                                      tGGGAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGa          >  1:680207/1‑101 (MQ=255)

                                                                                         gAACGATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTg       <  1:679814/101‑1 (MQ=255)

                                                                                             gATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGat   <  2:679706/101‑1 (MQ=255)

                                                                                             gATGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGat   <  2:679678/101‑1 (MQ=255)

                                                                                              aTGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGata  <  2:679835/101‑1 (MQ=255)

                                                                                              aTGCTCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGata  >  2:680210/1‑101 (MQ=255)

GATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATA  >  NC_000913/366707‑366901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`