Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,151,400 T→C V244A (GTC→GCC)  fabG → 3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,151,4000TC99.7% 1384.7 / ‑4.7 332V244A (GTC→GCC) fabG3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (129/202);  total (129/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 332 total aligned reads displayed.

CGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGG  >  NC_000913/1151302‑1151497
                                                                                                  |                                                                                                 
 gcACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTg                                                                                                >  1:2115554/1‑101 (MQ=255)
  cacaGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTg                                                                                                >  2:2115560/1‑100 (MQ=255)
    caGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGAcc                                                                                             <  1:2115198/101‑1 (MQ=255)
    caGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGAcc                                                                                             <  1:2114894/101‑1 (MQ=255)
     aGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACcg                                                                                            <  2:2115219/101‑1 (MQ=255)
      ggAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACcgc                                                                                           <  2:2115307/101‑1 (MQ=255)
       gAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACcgcg                                                                                          >  1:2115563/1‑101 (MQ=255)
         aaTCGCGAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGAt                                                                                        <  1:2115238/101‑1 (MQ=255)
         aaTCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGAt                                                                                        <  1:2115075/101‑1 (MQ=255)
         aaTCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGAt                                                                                        <  2:2114970/101‑1 (MQ=255)
           tCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGAAGTACATGGCCTGACCGCGAttt                                                                                      <  2:2114880/101‑1 (MQ=255)
             gCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGc                                                                                    >  2:2115569/1‑101 (MQ=255)
             gCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGc                                                                                    >  1:2115570/1‑101 (MQ=255)
             gCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGc                                                                                    <  1:2114528/101‑1 (MQ=255)
               cAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGcac                                                                                  >  1:2115573/1‑101 (MQ=255)
                 aCGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACaa                                                                                <  1:2114771/101‑1 (MQ=255)
                  cgcgGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACaaa                                                                               <  1:2114976/101‑1 (MQ=255)
                    cgGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAAt                                                                             <  2:2114938/101‑1 (MQ=255)
                     ggTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATg                                                                            <  1:2115061/101‑1 (MQ=255)
                     ggTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATg                                                                            >  2:2115580/1‑101 (MQ=255)
                        tGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTc                                                                         >  1:2115583/1‑101 (MQ=255)
                        tGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTc                                                                         >  2:2115584/1‑101 (MQ=255)
                           aTTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATg                                                                      <  2:2114915/101‑1 (MQ=255)
                           aTTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATg                                                                      <  2:2114800/101‑1 (MQ=255)
                           aTTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATg                                                                      <  2:2115448/101‑1 (MQ=255)
                            ttCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGt                                                                     >  2:2115590/1‑101 (MQ=255)
                             tCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGtt                                                                    <  1:2115179/101‑1 (MQ=255)
                              ccTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTg                                                                   <  2:2115076/101‑1 (MQ=255)
                               cTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTgc                                                                  <  1:2115008/101‑1 (MQ=255)
                               cTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTgc                                                                  <  1:2115083/101‑1 (MQ=255)
                               cTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTgc                                                                  <  1:2115218/101‑1 (MQ=255)
                               cTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTgc                                                                  >  1:2115593/1‑101 (MQ=255)
                                tGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTgcg                                                                 >  2:2115596/1‑101 (MQ=255)
                                 ggCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTgcgc                                                                <  1:2114949/101‑1 (MQ=255)
                                 ggCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTgcgc                                                                <  1:2115128/101‑1 (MQ=255)
                                  gCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGAcc                                                                                             >  1:2115600/1‑71 (MQ=255)
                                  gCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTgcgcg                                                               <  1:2115102/101‑1 (MQ=255)
                                    aTCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCa                                                             >  2:2115603/1‑101 (MQ=255)
                                    aTCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCa                                                             >  1:2115605/1‑101 (MQ=255)
                                           gaAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCg                                                      >  1:2115613/1‑101 (MQ=255)
                                           gaAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCg                                                      >  2:2115611/1‑101 (MQ=255)
                                            aaGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGt                                                     >  2:2115616/1‑101 (MQ=255)
                                              gcagcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTgg                                                   <  2:2115148/101‑1 (MQ=255)
                                               cagcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGt                                                  >  1:2115622/1‑101 (MQ=255)
                                               cagcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGt                                                  <  2:2114954/101‑1 (MQ=255)
                                               cagcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGt                                                  >  2:2115620/1‑101 (MQ=255)
                                               cagcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGt                                                  <  1:2115114/101‑1 (MQ=255)
                                                agcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGtt                                                 <  2:2115321/101‑1 (MQ=255)
                                                 gcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTa                                                <  2:2115186/101‑1 (MQ=255)
                                                   ttACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                              <  2:2115253/101‑1 (MQ=255)
                                                   ttACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                              <  2:2115266/101‑1 (MQ=255)
                                                   ttACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                              <  1:2115277/101‑1 (MQ=255)
                                                   ttACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                              <  1:2114834/101‑1 (MQ=255)
                                                   ttACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                              >  1:2115628/1‑101 (MQ=255)
                                                    tACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGa                                             >  1:2115631/1‑101 (MQ=255)
                                                     aCATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAg                                            <  2:2115346/101‑1 (MQ=255)
                                                        tCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaa                                         >  2:2115634/1‑101 (MQ=255)
                                                        tCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaa                                         <  2:2114985/101‑1 (MQ=255)
                                                         cACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaat                                        >  2:2115637/1‑101 (MQ=255)
                                                          aCGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaata                                       <  2:2115093/101‑1 (MQ=255)
                                                          aCGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaata                                       >  1:2115640/1‑101 (MQ=255)
                                                           cGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaataa                                      <  2:2115287/101‑1 (MQ=255)
                                                            gggTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTaataat                                     <  1:2115157/101‑1 (MQ=255)
                                                            gggTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTaataat                                     <  2:2115116/101‑1 (MQ=255)
                                                            gggTGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTaataat                                     >  2:2115645/1‑101 (MQ=255)
                                                               tGAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAg                                  >  1:2115648/1‑101 (MQ=255)
                                                                gAAACTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGt                                 <  1:2115132/101‑1 (MQ=255)
                                                                 aaaCTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTg                                <  2:2115065/101‑1 (MQ=255)
                                                                  aaCTTTGCATGTGAACGGCGGGATGTACATGGCCTGACcgc                                                                                           >  2:2115650/1‑41 (MQ=255)
                                                                   aCTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCa                              <  2:2115050/101‑1 (MQ=255)
                                                                   aCTTTGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCa                              <  1:2115371/101‑1 (MQ=255)
                                                                     tttGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCaaa                            >  1:2115655/1‑101 (MQ=255)
                                                                     tttGCATGTGAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCaaa                            <  1:2115037/101‑1 (MQ=255)
                                                                           tgtgAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAtt                      >  2:2115658/1‑101 (MQ=255)
                                                                           tgtgAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAtt                      >  1:2115659/1‑101 (MQ=255)
                                                                            gtgAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAttt                     <  1:2115139/101‑1 (MQ=255)
                                                                            gtgAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAttt                     <  1:2115300/101‑1 (MQ=255)
                                                                            gtgAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAttt                     <  2:2114998/101‑1 (MQ=255)
                                                                            gtgAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAttt                     <  2:2115481/101‑1 (MQ=255)
                                                                             tgAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTg                    >  2:2115663/1‑101 (MQ=255)
                                                                              gAACGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGc                   <  1:2115267/101‑1 (MQ=255)
                                                                               aaCGGCGGGGTGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCg                  >  2:2115668/1‑101 (MQ=255)
                                                                               aaCGGCGGGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCg                  <  1:2115536/101‑1 (MQ=255)
                                                                                 cggcggGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGtt                <  2:2114847/101‑1 (MQ=255)
                                                                                  ggcggGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTa               >  1:2115672/1‑101 (MQ=255)
                                                                                  ggcggGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTa               <  2:2115207/101‑1 (MQ=255)
                                                                                   gcggGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTAt              >  2:2115675/1‑101 (MQ=255)
                                                                                    cggGATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTAtt             <  1:2115315/101‑1 (MQ=255)
                                                                                     gggATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTg            >  1:2115678/1‑101 (MQ=255)
                                                                                      ggATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgg           <  1:2115341/101‑1 (MQ=255)
                                                                                      ggATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgg           >  2:2115680/1‑101 (MQ=255)
                                                                                       gATGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTggg          <  2:2115105/101‑1 (MQ=255)
                                                                                        aTGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgggg         <  2:2115416/101‑1 (MQ=255)
                                                                                        aTGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgggg         <  1:2115145/101‑1 (MQ=255)
                                                                                        aTGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgggg         <  1:2115467/101‑1 (MQ=255)
                                                                                         tGTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTggggg        <  1:2115205/101‑1 (MQ=255)
                                                                                          gTACATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgggggg       >  1:2115682/1‑101 (MQ=255)
                                                                                             cATGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTaa    <  2:2115174/101‑1 (MQ=255)
                                                                                               tGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAgg  <  2:2115123/101‑1 (MQ=255)
                                                                                               tGGCCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAgg  <  2:2115018/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGG  >  NC_000913/1151302‑1151497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑