BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	148,360	C→T	A146T (GCC→ACC)	panC ←	pantothenate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	148,360	0	C	T	99.7%	1285.4 / NA	317	A146T (GCC→ACC)	panC	pantothenate synthetase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (173/143); minor base G (1/0); total (174/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 317 total aligned reads displayed.

CATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGG  >  NC_000913/148262‑148457

cATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGa                                                                                                 <  1:274110/101‑1 (MQ=255)

cATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGa                                                                                                 >  1:274780/1‑101 (MQ=255)

 aTAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGAt                                                                                                >  2:274786/1‑101 (MQ=255)

   aaTTGGCACACCGGCAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGt                                                                                              >  2:274792/1‑101 (MQ=255)

   aaTTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGt                                                                                              >  1:274790/1‑101 (MQ=255)

   aaTTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGt                                                                                              >  1:274794/1‑101 (MQ=255)

      tGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCg                                                                                           <  1:274272/101‑1 (MQ=255)

       ggCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCgg                                                                                          >  1:274799/1‑101 (MQ=255)

        gCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGc                                                                                         >  2:274801/1‑101 (MQ=255)

         cacaCCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCt                                                                                        <  2:273982/101‑1 (MQ=255)

         cacaCCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCt                                                                                        >  1:274803/1‑101 (MQ=255)

           cacCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTgg                                                                                      <  1:274182/101‑1 (MQ=255)

            acCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGa                                                                                     >  2:274808/1‑101 (MQ=255)

             ccGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGAc                                                                                    <  2:274139/101‑1 (MQ=255)

               gACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCa                                                                                  <  1:274476/101‑1 (MQ=255)

                aCAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAg                                                                                 <  1:274125/101‑1 (MQ=255)

                   aTCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGtt                                                                              <  1:273676/101‑1 (MQ=255)

                   aTCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGtt                                                                              >  2:274812/1‑101 (MQ=255)

                   aTCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGtt                                                                              <  2:274097/101‑1 (MQ=255)

                   aTCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGtt                                                                              <  1:273974/101‑1 (MQ=255)

                     ctcAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGa                                                                            <  2:274167/101‑1 (MQ=255)

                     ctcAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGa                                                                            <  1:274150/101‑1 (MQ=255)

                        aaTATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACa                                                                         >  1:274816/1‑101 (MQ=255)

                         atatCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAg                                                                        >  2:274818/1‑101 (MQ=255)

                         atatCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAg                                                                        >  1:274820/1‑101 (MQ=255)

                            tCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCtt                                                                     <  2:274448/101‑1 (MQ=255)

                            tCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCtt                                                                     >  1:274824/1‑101 (MQ=255)

                             cGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCtag                                                                    >  2:274830/1‑99 (MQ=255)

                             cGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTg                                                                    >  1:274828/1‑101 (MQ=255)

                             cGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTg                                                                    <  2:274663/101‑1 (MQ=255)

                                aGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTg                                                                 <  2:274403/101‑1 (MQ=255)

                                aGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTg                                                                 >  1:274832/1‑101 (MQ=255)

                                  cccATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGAc                                                               >  2:274836/1‑101 (MQ=255)

                                   ccATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACa                                                              >  1:274838/1‑101 (MQ=255)

                                      tatCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATa                                                           >  1:274842/1‑101 (MQ=255)

                                       atCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAg                                                          >  2:274845/1‑101 (MQ=255)

                                       atCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAg                                                          <  2:274349/101‑1 (MQ=255)

                                        tCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGt                                                         >  2:274850/1‑101 (MQ=255)

                                          ggCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCg                                                       >  1:274852/1‑101 (MQ=255)

                                           gCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGa                                                      >  1:274855/1‑101 (MQ=255)

                                            cAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGaa                                                     >  1:274858/1‑101 (MQ=255)

                                            cAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGaa                                                     <  2:274333/101‑1 (MQ=255)

                                             aaCCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTg                                                                    >  2:274860/1‑85 (MQ=255)

                                                cATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAAcgc                                                 >  1:274863/1‑101 (MQ=255)

                                                cATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAAcgc                                                 <  2:274397/101‑1 (MQ=255)

                                                 aTTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAAcgcc                                                <  1:274289/101‑1 (MQ=255)

                                                  ttttGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAAcgccg                                               <  2:274385/101‑1 (MQ=255)

                                                   tttGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAAcgccgc                                              <  2:274375/101‑1 (MQ=255)

                                                   tttGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAAcgccgc                                              <  1:274321/101‑1 (MQ=255)

                                                    ttGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCcgcg                                             <  1:274257/101‑1 (MQ=255)

                                                     tGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGTAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGa                                            >  1:274869/1‑101 (MQ=255)

                                                     tGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGa                                            >  2:274868/1‑101 (MQ=255)

                                                       cGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGaaa                                          <  1:274363/101‑1 (MQ=255)

                                                        ggATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGaaaa                                         >  2:274875/1‑101 (MQ=255)

                                                          aTCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATg                                       >  1:274877/1‑101 (MQ=255)

                                                           tCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGt                                      >  1:274881/1‑101 (MQ=255)

                                                           tCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGt                                      <  2:274239/101‑1 (MQ=255)

                                                           tCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGt                                      <  1:274546/101‑1 (MQ=255)

                                                            cAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTc                                     >  2:274884/1‑101 (MQ=255)

                                                             aGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTcc                                    >  1:274886/1‑101 (MQ=255)

                                                               cgcCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCg                                  <  1:274034/101‑1 (MQ=255)

                                                               cgcCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCg                                  >  2:274891/1‑101 (MQ=255)

                                                                gcCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCgg                                 >  1:274895/1‑101 (MQ=255)

                                                                gcCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCgg                                 >  2:274894/1‑101 (MQ=255)

                                                                  cAGTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGAc                               >  1:274899/1‑101 (MQ=255)

                                                                    gTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACgg                             <  2:274337/101‑1 (MQ=255)

                                                                    gTTGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACgg                             <  2:274428/101‑1 (MQ=255)

                                                                      tGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCt                           <  1:274489/101‑1 (MQ=255)

                                                                      tGCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCt                           <  2:274505/101‑1 (MQ=255)

                                                                       gCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTg                          <  1:274341/101‑1 (MQ=255)

                                                                       gCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTg                          >  1:274904/1‑101 (MQ=255)

                                                                       gCTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTg                          >  2:274903/1‑101 (MQ=255)

                                                                        cTGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTgg                         <  2:274462/101‑1 (MQ=255)

                                                                         tGAAAATCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGc                        >  2:274909/1‑101 (MQ=255)

                                                                           aaaaTCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCAc                      >  1:274913/1‑101 (MQ=255)

                                                                            aaaTCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCAcc                     >  1:274917/1‑101 (MQ=255)

                                                                             aaTCTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCt                    >  2:274919/1‑101 (MQ=255)

                                                                                cTTTTTCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTcc                 >  1:274922/1‑101 (MQ=255)

                                                                                 tttttCACCGAAACAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCgg                                 >  1:274925/1‑84 (MQ=255)

                                                                                   tttCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGc              >  1:274928/1‑101 (MQ=255)

                                                                                    ttCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCa             >  2:274931/1‑101 (MQ=255)

                                                                                     tCACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCAt            >  2:274934/1‑101 (MQ=255)

                                                                                      cACCGAAGGAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCa                       <  1:274393/89‑1 (MQ=255)

                                                                                      cACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGaaaa                                         >  2:274940/1‑71 (MQ=255)

                                                                                      cACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTg                          >  1:274939/1‑86 (MQ=255)

                                                                                      cACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATg           <  2:274359/101‑1 (MQ=255)

                                                                                      cACCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATg           <  2:274292/101‑1 (MQ=255)

                                                                                       aCCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATgg          <  1:274460/101‑1 (MQ=255)

                                                                                       aCCGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATgg          <  1:274226/101‑1 (MQ=255)

                                                                                        ccGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGt         <  1:274299/101‑1 (MQ=255)

                                                                                         cGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTc        >  1:274944/1‑101 (MQ=255)

                                                                                         cGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTc        <  1:274735/101‑1 (MQ=255)

                                                                                         cGAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTc        >  1:274947/1‑101 (MQ=255)

                                                                                          gAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCg       <  1:274603/101‑1 (MQ=255)

                                                                                          gAAGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGAGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCg       <  1:274569/101‑1 (MQ=255)

                                                                                           aaGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGa      <  1:274376/101‑1 (MQ=255)

                                                                                            aGCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGaa     >  2:274950/1‑101 (MQ=255)

                                                                                             gCAGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGaaa    <  1:274435/101‑1 (MQ=255)

                                                                                               aGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAgg  <  2:274514/101‑1 (MQ=255)

                                                                                               aGGTGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAgg  >  1:274954/1‑101 (MQ=255)

CATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGG  >  NC_000913/148262‑148457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`