Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,682,027 T→C D240G (GAC→GGC)  yphG ← DUF5107 domain‑containing protein YphG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,682,0270TC99.7% 1398.1 / NA 328D240G (GAC→GGC) yphGDUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (155/172);  minor base A (1/0);  total (156/172)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 331 total aligned reads displayed.

CGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATC  >  NC_000913/2681929‑2682124
                                                                                                  |                                                                                                 
cGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGccc                                                                                                 >  1:4976854/1‑101 (MQ=255)
 gCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCa                                                                                                >  2:4976858/1‑101 (MQ=255)
  ccATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCAc                                                                                               >  1:4976860/1‑101 (MQ=255)
  ccATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCAc                                                                                               <  2:4976349/101‑1 (MQ=255)
    aTCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTc                                                                                             <  1:4976330/101‑1 (MQ=255)
     tCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTcc                                                                                            <  1:4976310/101‑1 (MQ=255)
     tCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTcc                                                                                            <  1:4976391/101‑1 (MQ=255)
      cTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCa                                                                                           <  1:4976351/101‑1 (MQ=255)
        tCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGc                                                                                         >  2:4976864/1‑101 (MQ=255)
         cATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCg                                                                                        <  2:4976650/101‑1 (MQ=255)
          aTCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCgg                                                                                       >  1:4976868/1‑101 (MQ=255)
           tCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGa                                                                                      <  1:4976585/101‑1 (MQ=255)
            cGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGag                                                                                     <  1:4976190/101‑1 (MQ=255)
                acacCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGagtagt                                                                                 >  2:4976873/1‑101 (MQ=255)
                 caccacGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTc                                                                                >  1:4976874/1‑101 (MQ=255)
                  accacGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTcc                                                                               <  1:4976383/101‑1 (MQ=255)
                   ccacGCGCCGTCAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCa                                                                              <  1:4976482/101‑1 (MQ=255)
                     acGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACt                                                                            <  1:4976273/101‑1 (MQ=255)
                       gcgcCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACttt                                                                          >  2:4976877/1‑101 (MQ=255)
                           cGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTgtag                                                                      >  2:4976881/1‑101 (MQ=255)
                           cGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTgtag                                                                      <  1:4976005/101‑1 (MQ=255)
                              cAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTaa                                                                   >  1:4976886/1‑101 (MQ=255)
                              cAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTaa                                                                   <  1:4976569/101‑1 (MQ=255)
                                aaaTCGTACTGCGATTTTTCGTCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt                                                                 <  2:4976540/101‑1 (MQ=255)
                                aaaTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt                                                                 <  1:4976427/101‑1 (MQ=255)
                                 aaTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTg                                                                >  1:4976890/1‑101 (MQ=255)
                                  aTCGTACTGCGATTTTTCGTCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGc                                                               <  1:4976248/101‑1 (MQ=255)
                                   tCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGcc                                                              <  1:4976371/101‑1 (MQ=255)
                                     gTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCgg                                                            >  2:4976893/1‑101 (MQ=255)
                                      tACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGt                                                           >  1:4976897/1‑101 (MQ=255)
                                       aCTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTa                                                          >  2:4976898/1‑101 (MQ=255)
                                       aCTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTa                                                          <  2:4976525/101‑1 (MQ=255)
                                       aCTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTa                                                          <  2:4976432/101‑1 (MQ=255)
                                        cTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAg                                                         <  2:4976474/101‑1 (MQ=255)
                                           cGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGa                                                      <  1:4976080/101‑1 (MQ=255)
                                           cGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGa                                                      <  1:4976473/101‑1 (MQ=255)
                                            gATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGAt                                                     <  2:4976382/101‑1 (MQ=255)
                                            gATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGAt                                                     >  2:4976901/1‑101 (MQ=255)
                                             aTTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTgtag                                                                      >  2:4976907/1‑83 (MQ=255)
                                              tttttCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATgg                                                   >  1:4976910/1‑101 (MQ=255)
                                               ttttCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATggg                                                  <  2:4976294/101‑1 (MQ=255)
                                               ttttCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATggg                                                  >  1:4976913/1‑101 (MQ=255)
                                                tttCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATgggg                                                 >  2:4976917/1‑101 (MQ=255)
                                                tttCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATgggg                                                 <  2:4976160/101‑1 (MQ=255)
                                                 ttCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGa                                                >  1:4976918/1‑101 (MQ=255)
                                                     gCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGc                                            <  2:4976253/101‑1 (MQ=255)
                                                      ccATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGAt                                                     <  2:4976218/91‑1 (MQ=255)
                                                      ccATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGaa                                               <  2:4976399/97‑1 (MQ=255)
                                                      ccATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCg                                           >  1:4976923/1‑101 (MQ=255)
                                                       cATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAg                                             <  1:4976494/98‑1 (MQ=255)
                                                       cATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCgg                                          >  2:4976929/1‑101 (MQ=255)
                                                       cATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCgg                                          >  1:4976926/1‑101 (MQ=255)
                                                        atatatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGga                                         <  1:4976440/101‑1 (MQ=255)
                                                         tatatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGaa                                               <  1:4976214/94‑1 (MQ=255)
                                                          atatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGgaga                                       >  2:4976934/1‑101 (MQ=255)
                                                          atatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGACCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGgaga                                       >  1:4976933/1‑101 (MQ=255)
                                                           tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGAc                                      <  2:4976553/101‑1 (MQ=255)
                                                           tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGAc                                      <  2:4976445/101‑1 (MQ=255)
                                                           tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGAc                                      <  1:4976458/101‑1 (MQ=255)
                                                           tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGAc                                      <  2:4976419/101‑1 (MQ=255)
                                                            atGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACg                                     <  1:4976628/101‑1 (MQ=255)
                                                            atGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACg                                     <  2:4976501/101‑1 (MQ=255)
                                                            atGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACg                                     <  2:4976324/101‑1 (MQ=255)
                                                             tGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACgg                                    >  1:4976940/1‑101 (MQ=255)
                                                              gAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGc                                   >  2:4976942/1‑101 (MQ=255)
                                                               aGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGcc                                  >  2:4976947/1‑101 (MQ=255)
                                                               aGGTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGcc                                  >  1:4976949/1‑101 (MQ=255)
                                                                ggTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGccc                                 >  1:4976952/1‑101 (MQ=255)
                                                                 gTTGGAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCg                                >  2:4976954/1‑101 (MQ=255)
                                                                    ggAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGttt                             <  1:4976292/101‑1 (MQ=255)
                                                                    ggAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGttt                             >  1:4976961/1‑101 (MQ=255)
                                                                    ggAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGttt                             >  2:4976962/1‑101 (MQ=255)
                                                                    ggAACAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGttt                             <  2:4976486/101‑1 (MQ=255)
                                                                      aaCAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACgg                                    >  1:4976966/1‑92 (MQ=255)
                                                                      aaCAGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGc                           <  2:4976087/101‑1 (MQ=255)
                                                                         aGGCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGt                        >  2:4976968/1‑101 (MQ=255)
                                                                           gCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCg                                                       >  2:4976971/1‑68 (MQ=255)
                                                                           gCACATTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGa                      <  1:4976524/101‑1 (MQ=255)
                                                                            cacaTTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGAt                     <  2:4976441/101‑1 (MQ=255)
                                                                              caTTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGc                                   >  1:4976976/1‑85 (MQ=255)
                                                                               aTTTTTATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCaa                  <  2:4976612/101‑1 (MQ=255)
                                                                                tttttATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTg                       >  2:4976978/1‑95 (MQ=255)
                                                                                 ttttATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAac                <  1:4976511/101‑1 (MQ=255)
                                                                                  tttATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAaca               >  1:4976983/1‑101 (MQ=255)
                                                                                  tttATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAaca               <  2:4976358/101‑1 (MQ=255)
                                                                                  tttATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAaca               <  1:4976465/101‑1 (MQ=255)
                                                                                   ttATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAacac              >  1:4976987/1‑101 (MQ=255)
                                                                                   ttATAGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAacac              <  2:4976720/101‑1 (MQ=255)
                                                                                    tataGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAAGAcc             <  2:4976413/101‑1 (MQ=255)
                                                                                    tataGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACAcc             <  2:4976572/101‑1 (MQ=255)
                                                                                       aGCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACAccgcc          >  1:4976990/1‑101 (MQ=255)
                                                                                        gCGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACAccgccg         >  1:4976993/1‑101 (MQ=255)
                                                                                         cGAGAAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGt        >  2:4976994/1‑101 (MQ=255)
                                                                                          gagaAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGtt       >  1:4977001/1‑101 (MQ=255)
                                                                                          gagaAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGtt       >  1:4976998/1‑101 (MQ=255)
                                                                                           agaAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTa      <  2:4976622/101‑1 (MQ=255)
                                                                                            gaAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTAc     >  1:4977008/1‑101 (MQ=255)
                                                                                            gaAATGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTAc     >  2:4977006/1‑101 (MQ=255)
                                                                                              aaTGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACAt   >  1:4977013/1‑101 (MQ=255)
                                                                                               aTGCCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATc  <  1:4976672/101‑1 (MQ=255)
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CGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATC  >  NC_000913/2681929‑2682124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑