Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,954,256 T→C N583S (AAC→AGC)  recB ← exodeoxyribonuclease V subunit RecB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,954,2560TC100.0% 1587.4 / NA 372N583S (AAC→AGC) recBexodeoxyribonuclease V subunit RecB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (216/156);  total (216/156)

Only 100 of 372 total aligned reads displayed.

GCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGTTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGC  >  NC_000913/2954158‑2954353
                                                                                                  |                                                                                                 
  gcACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGa                                                                                               >  1:5477860/1‑101 (MQ=255)
    aCTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGaaa                                                                                             >  2:5477864/1‑101 (MQ=255)
     cTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAg                                                                                            >  1:5477868/1‑101 (MQ=255)
       aCGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGaaa                                                                                             >  2:5477871/1‑98 (MQ=255)
        cGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGaa                                                                                              <  2:5477174/96‑1 (MQ=255)
        cGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTa                                                                                         >  1:5477872/1‑101 (MQ=255)
         gCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTaa                                                                                        <  1:5476909/101‑1 (MQ=255)
         gCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTaa                                                                                        <  1:5477308/101‑1 (MQ=255)
         gCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTaa                                                                                        <  1:5477335/101‑1 (MQ=255)
          cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTaaa                                                                                       <  2:5477326/101‑1 (MQ=255)
          cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTaaa                                                                                       <  2:5477221/101‑1 (MQ=255)
          cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTaaa                                                                                       <  1:5477356/101‑1 (MQ=255)
          cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTaaa                                                                                       <  1:5477247/101‑1 (MQ=255)
           aGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAAc                                                                                      <  1:5477572/101‑1 (MQ=255)
           aGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAAc                                                                                      <  1:5477601/101‑1 (MQ=255)
            gggTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACg                                                                                     >  1:5477880/1‑101 (MQ=255)
              gtgtTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGa                                                                                   >  2:5477885/1‑101 (MQ=255)
               tgtTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGaa                                                                                              >  2:5477888/1‑89 (MQ=255)
               tgtTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGaa                                                                                  <  2:5477465/101‑1 (MQ=255)
                  tctcACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAggga                                                                                         >  1:5477892/1‑89 (MQ=255)
                   ctcACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGa                                                                              >  2:5477894/1‑101 (MQ=255)
                    tcACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGAt                                                                             >  1:5477897/1‑101 (MQ=255)
                     cACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGAtt                                                                            <  1:5477407/101‑1 (MQ=255)
                     cACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGAtt                                                                            >  1:5477902/1‑101 (MQ=255)
                      aCGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGAttt                                                                           <  1:5477530/101‑1 (MQ=255)
                        gTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTaaa                                                                                       >  2:5477909/1‑87 (MQ=255)
                        gTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTcc                                                                         <  1:5477438/101‑1 (MQ=255)
                        gTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTcc                                                                         >  1:5477907/1‑101 (MQ=255)
                         ttCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCa                                                                        <  2:5477499/101‑1 (MQ=255)
                         ttCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCa                                                                        <  2:5477589/101‑1 (MQ=255)
                          tCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAg                                                                       <  1:5477363/101‑1 (MQ=255)
                           cGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGc                                                                      <  2:5477382/101‑1 (MQ=255)
                            gggCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTc                                                                                                 >  2:5477916/1‑73 (MQ=255)
                            gggCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTcc                                                                         >  1:5477915/1‑97 (MQ=255)
                               cGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACg                                                                  <  1:5477627/101‑1 (MQ=255)
                               cGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACg                                                                  >  1:5477920/1‑101 (MQ=255)
                                 tcatcaCCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGtt                                                                <  2:5477561/101‑1 (MQ=255)
                                   atcaCCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTaa                                                              <  2:5477270/101‑1 (MQ=255)
                                    tcaCCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAg                                                             >  1:5477929/1‑101 (MQ=255)
                                    tcaCCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAg                                                             <  1:5477541/101‑1 (MQ=255)
                                    tcaCCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAg                                                             >  2:5477926/1‑101 (MQ=255)
                                     caCCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGCAAACgg                                                                                    >  2:5477933/1‑77 (MQ=255)
                                       ccGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCa                                                          >  1:5477936/1‑101 (MQ=255)
                                         gCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATc                                                        >  2:5477941/1‑101 (MQ=255)
                                          ccTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATcg                                                       >  2:5477944/1‑101 (MQ=255)
                                           cTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATcgc                                                      >  1:5477946/1‑101 (MQ=255)
                                             gCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCgcgc                                                    <  2:5477413/101‑1 (MQ=255)
                                             gCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCgcgc                                                    >  2:5477951/1‑101 (MQ=255)
                                              caacaaCCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCa                                                   <  1:5477385/101‑1 (MQ=255)
                                              caacaaCCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCa                                                   >  1:5477953/1‑101 (MQ=255)
                                               aacaacCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCAc                                                  <  2:5477353/101‑1 (MQ=255)
                                               aacaacCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCAc                                                  >  2:5477960/1‑101 (MQ=255)
                                                acaacCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAgg                                                                                           >  1:5477962/1‑59 (MQ=255)
                                                 caacCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCt                                                <  1:5477505/101‑1 (MQ=255)
                                                  aacCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATcg                                                       >  2:5477967/1‑93 (MQ=255)
                                                   acCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATcgc                                                      >  1:5477970/1‑93 (MQ=255)
                                                   acCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTgg                                              <  2:5477304/101‑1 (MQ=255)
                                                     cAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGggc                                            <  2:5477635/101‑1 (MQ=255)
                                                      aaaGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCAt                                                         >  1:5477976/1‑87 (MQ=255)
                                                      aaaGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCAcc                                                 >  2:5477975/1‑95 (MQ=255)
                                                        aGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCgcgc                                                    >  1:5477983/1‑90 (MQ=255)
                                                         gCATTTCCTGTGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGcc                                        <  1:5477401/101‑1 (MQ=255)
                                                         gCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGcc                                        >  2:5477984/1‑101 (MQ=255)
                                                            tttCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGcctcc                                     >  1:5477993/1‑101 (MQ=255)
                                                            tttCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGcctcc                                     >  2:5477990/1‑101 (MQ=255)
                                                             ttCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCGAGCAACGTTAAGGCATCGCGCACCTGGGCGGcctcct                                    <  1:5477482/101‑1 (MQ=255)
                                                             ttCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGGAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGcctcct                                    >  1:5477996/1‑101 (MQ=255)
                                                              tCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTg                                   <  2:5477461/101‑1 (MQ=255)
                                                              tCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTg                                   <  1:5477133/101‑1 (MQ=255)
                                                              tCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGGAAAAGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTggg                                             >  2:5477999/1‑91 (MQ=255)
                                                               ccTGCGCTTCCAGAGCTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAg                                                             >  1:5478002/1‑74 (MQ=255)
                                                                cTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTggc                                 <  1:5477425/101‑1 (MQ=255)
                                                                  gcgcTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTggcgg                               <  1:5477473/101‑1 (MQ=255)
                                                                   cgcTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTggcggc                              >  2:5478007/1‑101 (MQ=255)
                                                                    gcTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCt                             <  1:5477514/101‑1 (MQ=255)
                                                                    gcTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCt                             <  1:5477672/101‑1 (MQ=255)
                                                                     cTTCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTg                            >  2:5478013/1‑101 (MQ=255)
                                                                      ttCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTgc                           <  1:5477269/101‑1 (MQ=255)
                                                                      ttCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTgc                           >  1:5478014/1‑101 (MQ=255)
                                                                       tCCAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTgcg                          <  2:5477545/101‑1 (MQ=255)
                                                                         cAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTggc                                 >  2:5478023/1‑92 (MQ=255)
                                                                         cAGAGTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCa                        >  1:5478021/1‑101 (MQ=255)
                                                                          agagTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCAc                       >  1:5478026/1‑101 (MQ=255)
                                                                           gagTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGAttt                                                                           >  2:5478033/1‑48 (MQ=255)
                                                                           gagTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCAcc                      >  1:5478032/1‑101 (MQ=255)
                                                                            agTTTCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCa                     <  1:5477217/101‑1 (MQ=255)
                                                                                tCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGcctcc                                     >  2:5478042/1‑81 (MQ=255)
                                                                                tCAAAAACACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGcac                 >  1:5478039/1‑101 (MQ=255)
                                                                                  aaaaaCACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCt                                                >  1:5478052/1‑68 (MQ=255)
                                                                                  aaaaaCACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGcc                                        >  2:5478051/1‑76 (MQ=255)
                                                                                  aaaaaCACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCacac               >  1:5478047/1‑101 (MQ=255)
                                                                                    aaaCACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTggcgg                               >  2:5478058/1‑83 (MQ=255)
                                                                                     aaCACTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGa            >  1:5478059/1‑101 (MQ=255)
                                                                                      acacTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGAt           <  2:5477606/101‑1 (MQ=255)
                                                                                        acTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGt         <  2:5477520/101‑1 (MQ=255)
                                                                                         cTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTggcggc                              >  2:5478068/1‑79 (MQ=255)
                                                                                         cTGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTc        >  1:5478065/1‑101 (MQ=255)
                                                                                          tGTCGCGGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTcc       >  1:5478071/1‑101 (MQ=255)
                                                                                              gcgGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAg   >  2:5478076/1‑101 (MQ=255)
                                                                                               cgGCTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAgc  >  1:5478080/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAACACTGTCGCGGTTCGAAAGGTAAACGGAAGGGATTTCCAGCAACGTTAAGGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGC  >  NC_000913/2954158‑2954353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑