Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,321,233 C→A L127F (TTG→TTT)  yhbX ← putative hydrolase, inner membrane

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,321,2330CA100.0% 1795.8 / NA 410L127F (TTG→TTT) yhbXputative hydrolase, inner membrane
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (249/161);  total (249/161)

Only 100 of 424 total aligned reads displayed.

TGACAAGCGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCCAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAGCACACTAA  >  NC_000913/3321135‑3321330
                                                                                                  |                                                                                                 
tGACAAGCGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCaaa                                                                                                 >  2:6172508/1‑101 (MQ=255)
tGACAAGCGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCaaa                                                                                                 <  2:6171867/101‑1 (MQ=255)
  aCAAGCGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAaaaa                                                                                               >  1:6172515/1‑101 (MQ=255)
  aCAAGCGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAaaaa                                                                                               <  1:6172258/101‑1 (MQ=255)
   cAAGCGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCaaaaaa                                                                                              <  2:6172417/101‑1 (MQ=255)
       cGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAaaga                                                                                          <  2:6171933/101‑1 (MQ=255)
       cGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAaaga                                                                                          <  2:6172016/101‑1 (MQ=255)
       cGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAaaga                                                                                          <  1:6172192/101‑1 (MQ=255)
       cGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCAC‑TTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAaagaa                                                                                         >  2:6172519/1‑101 (MQ=255)
        ggAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAaagaa                                                                                         >  1:6172521/1‑101 (MQ=255)
          aaaaTAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAgg                                                                                       <  2:6172062/101‑1 (MQ=255)
          aaaaTAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAgg                                                                                       >  2:6172525/1‑101 (MQ=255)
           aaaTAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAggg                                                                                      <  2:6171965/101‑1 (MQ=255)
            aaTAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGa                                                                                     >  2:6172530/1‑101 (MQ=255)
             aTAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAggg                                                                                      >  2:6172537/1‑99 (MQ=255)
             aTAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGAt                                                                                    <  1:6172202/101‑1 (MQ=255)
              tAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATa                                                                                   >  1:6172539/1‑101 (MQ=255)
               aaaCTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATaa                                                                                  <  2:6171914/101‑1 (MQ=255)
               aaaCTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATaa                                                                                  <  1:6172165/101‑1 (MQ=255)
               aaaCTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATaa                                                                                  >  1:6172544/1‑101 (MQ=255)
                aaCTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATaaa                                                                                 <  1:6172293/101‑1 (MQ=255)
                  cTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATaaaaa                                                                               >  2:6172547/1‑101 (MQ=255)
                   tGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATaaaaaa                                                                              <  1:6172081/101‑1 (MQ=255)
                    gCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAg                                                                             >  1:6172554/1‑101 (MQ=255)
                    gCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAg                                                                             <  2:6171895/101‑1 (MQ=255)
                    gCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAg                                                                             <  2:6172194/101‑1 (MQ=255)
                     cccGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGc                                                                            >  2:6172556/1‑101 (MQ=255)
                      ccGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCa                                                                           >  2:6172561/1‑101 (MQ=255)
                        gAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCaaa                                                                         >  2:6172566/1‑101 (MQ=255)
                        gAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCaaa                                                                         >  1:6172567/1‑101 (MQ=255)
                        gAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCaaa                                                                         <  1:6171948/101‑1 (MQ=255)
                         aaaTGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAg                                                                        >  2:6172573/1‑101 (MQ=255)
                          aaTGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAgg                                                                       >  2:6172579/1‑101 (MQ=255)
                          aaTGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAgg                                                                       >  1:6172580/1‑101 (MQ=255)
                             gACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGcac                                                                    >  2:6172588/1‑101 (MQ=255)
                              aCAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGcaca                                                                   <  2:6172249/101‑1 (MQ=255)
                              aCAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGcaca                                                                   >  1:6172590/1‑101 (MQ=255)
                                aaTCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATa                                                                 >  2:6172597/1‑101 (MQ=255)
                                aaTCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATa                                                                 >  1:6172596/1‑101 (MQ=255)
                                aaTCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATa                                                                 <  1:6172114/101‑1 (MQ=255)
                                    aGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATa                                                             <  1:6171618/101‑1 (MQ=255)
                                    aGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATa                                                             >  1:6172603/1‑101 (MQ=255)
                                    aGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGACACATAGATa                                                             <  1:6172044/101‑1 (MQ=255)
                                       aataatATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAgg                                                          >  2:6172607/1‑101 (MQ=255)
                                       aataatATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAgg                                                          <  1:6171985/101‑1 (MQ=255)
                                       aataatATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAgg                                                          >  1:6172610/1‑101 (MQ=255)
                                          aataTTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGat                                                       >  2:6172615/1‑101 (MQ=255)
                                          aataTTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGat                                                       >  1:6172620/1‑101 (MQ=255)
                                           atatTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGata                                                      >  1:6172628/1‑101 (MQ=255)
                                           atatTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGata                                                      >  2:6172625/1‑101 (MQ=255)
                                            tatTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGatat                                                     <  1:6172075/101‑1 (MQ=255)
                                             atTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGAtata                                                    >  2:6172633/1‑101 (MQ=255)
                                              ttCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATAc                                                   >  2:6172638/1‑101 (MQ=255)
                                               tCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACa                                                  >  1:6172639/1‑101 (MQ=255)
                                                ccTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACAt                                                 <  1:6171887/101‑1 (MQ=255)
                                                ccTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACAt                                                 <  2:6171827/101‑1 (MQ=255)
                                                 cTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATc                                                <  2:6172225/101‑1 (MQ=255)
                                                 cTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATc                                                >  2:6172644/1‑101 (MQ=255)
                                                     cACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCa                                            >  1:6172648/1‑101 (MQ=255)
                                                      aCTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAg                                           <  2:6172127/101‑1 (MQ=255)
                                                      aCTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAg                                           <  1:6171859/101‑1 (MQ=255)
                                                       cTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGc                                          >  2:6172653/1‑101 (MQ=255)
                                                       cTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGc                                          <  2:6172156/101‑1 (MQ=255)
                                                         ttttttGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCAt                                        >  1:6172658/1‑101 (MQ=255)
                                                          tttttGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCAtt                                       >  2:6172663/1‑101 (MQ=255)
                                                          tttttGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCAtt                                       >  1:6172668/1‑101 (MQ=255)
                                                           ttttGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCAttt                                      <  2:6171996/101‑1 (MQ=255)
                                                            tttGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCAtttt                                     >  2:6172670/1‑101 (MQ=255)
                                                             ttGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTg                                    >  1:6172675/1‑101 (MQ=255)
                                                              tGTCGGCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGa                                   >  2:6172677/1‑101 (MQ=255)
                                                                  ggCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAAc                               >  1:6172684/1‑101 (MQ=255)
                                                                   gCAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACt                              >  2:6172687/1‑101 (MQ=255)
                                                                    cAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGatat                                                     >  2:6172691/1‑77 (MQ=255)
                                                                    cAAGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACtt                             <  2:6172093/101‑1 (MQ=255)
                                                                      aGGAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCa                           >  1:6172698/1‑101 (MQ=255)
                                                                        gAAACATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATc                         <  1:6172005/101‑1 (MQ=255)
                                                                         aaaCATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATcc                        >  2:6172702/1‑101 (MQ=255)
                                                                         aaaCATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATcc                        >  1:6172704/1‑101 (MQ=255)
                                                                          aaCATCATATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCg                       >  2:6172707/1‑101 (MQ=255)
                                                                             atcatATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGAt                    >  1:6172713/1‑101 (MQ=255)
                                                                               catATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATATATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCa                  <  2:6172104/101‑1 (MQ=255)
                                                                               catATTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCa                  >  1:6172718/1‑101 (MQ=255)
                                                                                atatTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCAc                 >  2:6172723/1‑101 (MQ=255)
                                                                                atatTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCAc                 <  1:6172499/101‑1 (MQ=255)
                                                                                 tatTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCa                  <  2:6172035/99‑1 (MQ=255)
                                                                                 tatTTTATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCAct                >  1:6172725/1‑101 (MQ=255)
                                                                                   ttttATTACTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCActct              >  1:6172731/1‑101 (MQ=255)
                                                                                    tttATTATTACTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTg             <  1:6172133/101‑1 (MQ=255)
                                                                                     ttattattaCTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGa                                                        <  2:6171787/57‑1 (MQ=255)
                                                                                     ttattattaCTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTgg            >  1:6172737/1‑101 (MQ=255)
                                                                                     ttattattaCTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTgg            >  2:6172736/1‑101 (MQ=255)
                                                                                       attattaCTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAg          <  1:6171800/101‑1 (MQ=255)
                                                                                        ttattaCTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAGc         <  2:6171716/101‑1 (MQ=255)
                                                                                        ttattaCTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAGc         >  1:6172745/1‑101 (MQ=255)
                                                                                        ttattaCTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAGc         >  2:6172744/1‑101 (MQ=255)
                                                                                         tattaCTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAGca        <  1:6172026/101‑1 (MQ=255)
                                                                                          attaCTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAGcac       >  2:6172751/1‑101 (MQ=255)
                                                                                            taCTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAGCacac     >  1:6172757/1‑101 (MQ=255)
                                                                                              cTGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAGCACACTa   <  2:6172174/101‑1 (MQ=255)
                                                                                               tGCAAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAGCACACTaa  >  1:6172762/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TGACAAGCGGAAAATAAACTGCCCGAAATGACAATCAGCAATAATATTCCTGTCACTTTTTTTGTCGGCAAGGAAACATCATATTTTATTATTACTGCCAAAAAAAGAAGGGATAAAAAAGCAAAGGCACATAGATAAGGGATATACATCCCCAGCATTTTGACAACTTCATCCGGATCACTCTGGAGCACACTAA  >  NC_000913/3321135‑3321330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑