BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,457,030	T→C	G218G (GGT→GGC)	gspD →	Type II secretion system protein GspD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,457,030	0	T	C	100.0%	1400.0 / NA	326	G218G (GGT→GGC)	gspD	Type II secretion system protein GspD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (169/157); total (169/157)

Only 100 of 326 total aligned reads displayed.

TCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCAGCGCA  >  NC_000913/3456933‑3457127

tCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCaaa                                                                                                <  2:6420060/101‑1 (MQ=255)

tCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCaaa                                                                                                <  1:6419721/101‑1 (MQ=255)

tCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCaaa                                                                                                >  1:6420342/1‑101 (MQ=255)

 cATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCaaaa                                                                                               >  1:6420346/1‑101 (MQ=255)

  aTCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCaaaa                                                                                               >  1:6420351/1‑100 (MQ=255)

   tCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCCAAAGCCACGGCAAAAGc                                                                                             <  2:6419891/101‑1 (MQ=255)

    cGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGcc                                                                                            <  2:6420167/101‑1 (MQ=255)

     ggCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCa                                                                                           >  2:6420355/1‑101 (MQ=255)

        acaGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGAt                                                                                        <  2:6419946/101‑1 (MQ=255)

         caGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATg                                                                                       >  1:6420358/1‑101 (MQ=255)

            agaAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCa                                                                                    <  2:6419680/101‑1 (MQ=255)

            agaAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCa                                                                                    <  2:6419845/101‑1 (MQ=255)

              aaGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGc                                                                                  <  2:6419977/101‑1 (MQ=255)

              aaGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGc                                                                                  <  1:6420039/101‑1 (MQ=255)

              aaGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGc                                                                                  >  1:6420362/1‑101 (MQ=255)

               agcagcAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGcc                                                                                 <  2:6419980/101‑1 (MQ=255)

               agcagcAAATTATTCATCTGGAATATGCCTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGcc                                                                                 >  2:6420366/1‑101 (MQ=255)

                 cagcaAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCcct                                                                               >  1:6420368/1‑101 (MQ=255)

                  agcaAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCcctc                                                                              >  2:6420373/1‑101 (MQ=255)

                   gcaAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCcctcc                                                                             >  1:6420375/1‑101 (MQ=255)

                    caAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCcctcct                                                                            <  1:6419740/101‑1 (MQ=255)

                     aaaTTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCctcctc                                                                           >  1:6420379/1‑101 (MQ=255)

                     aaaTTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCctcctc                                                                           <  2:6419901/101‑1 (MQ=255)

                       attattCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCtctc                                                                         >  1:6420384/1‑101 (MQ=255)

                        ttattCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTcc                                                                        >  2:6420385/1‑101 (MQ=255)

                         tattCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCcg                                                                       <  1:6419527/101‑1 (MQ=255)

                         tattCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCcg                                                                       <  2:6419951/101‑1 (MQ=255)

                          attCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCcgc                                                                      <  2:6419995/101‑1 (MQ=255)

                          attCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCcgc                                                                      <  1:6419874/101‑1 (MQ=255)

                             cATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGaa                                                                   <  1:6419914/101‑1 (MQ=255)

                             cATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGaa                                                                   <  2:6419859/101‑1 (MQ=255)

                              aTCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAg                                                                  <  1:6419575/101‑1 (MQ=255)

                              aTCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAg                                                                  >  1:6420392/1‑101 (MQ=255)

                              aTCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAg                                                                  >  2:6420391/1‑101 (MQ=255)

                                   gAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATtgt                                                             >  1:6420401/1‑101 (MQ=255)

                                   gAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATtgt                                                             >  1:6420398/1‑101 (MQ=255)

                                   gAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATtgt                                                             <  2:6420035/101‑1 (MQ=255)

                                    aaTATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGATAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATtgtg                                                            >  2:6420402/1‑101 (MQ=255)

                                    aaTATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATtgtg                                                            <  1:6419895/101‑1 (MQ=255)

                                      tatGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGc                                                          >  1:6420405/1‑101 (MQ=255)

                                        tGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCgg                                                        <  2:6419870/101‑1 (MQ=255)

                                         gCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGa                                                       >  1:6420410/1‑101 (MQ=255)

                                           gTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATa                                                     >  2:6420412/1‑101 (MQ=255)

                                           gTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATa                                                     <  1:6420019/101‑1 (MQ=255)

                                            tCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATaa                                                    <  2:6419490/101‑1 (MQ=255)

                                            tCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATaa                                                    <  2:6419714/101‑1 (MQ=255)

                                            tCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATaa                                                    <  1:6419974/101‑1 (MQ=255)

                                            tCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATaa                                                    >  1:6420415/1‑101 (MQ=255)

                                              aGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGc                                                  <  1:6419921/101‑1 (MQ=255)

                                               gCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCg                                                 >  1:6420421/1‑101 (MQ=255)

                                                cGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGa                                                <  2:6419963/101‑1 (MQ=255)

                                                    aGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGGTa                                                     >  2:6420425/1‑92 (MQ=255)

                                                     gATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCaa                                           >  1:6420426/1‑101 (MQ=255)

                                                     gATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCaa                                           <  1:6419648/101‑1 (MQ=255)

                                                      aTCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAAc                                          >  2:6420430/1‑101 (MQ=255)

                                                       tctcGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAAct                                         <  2:6419724/101‑1 (MQ=255)

                                                       tctcGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAAct                                         >  1:6420431/1‑101 (MQ=255)

                                                        ctcGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAActc                                        >  2:6420435/1‑101 (MQ=255)

                                                          cGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCgg                                                        <  1:6419689/83‑1 (MQ=255)

                                                          cGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAActctc                                      <  1:6420074/101‑1 (MQ=255)

                                                          cGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAActctc                                      >  1:6420439/1‑101 (MQ=255)

                                                           gCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAActctct                                     >  2:6420442/1‑101 (MQ=255)

                                                           gCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAActctct                                     >  1:6420445/1‑101 (MQ=255)

                                                            ccGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCtt                                    <  1:6419840/101‑1 (MQ=255)

                                                            ccGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCtt                                    >  1:6420449/1‑101 (MQ=255)

                                                              gagaTTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTat                                  >  2:6420455/1‑101 (MQ=255)

                                                              gagaTTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTat                                  >  1:6420453/1‑101 (MQ=255)

                                                                gaTTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTatca                                >  1:6420460/1‑101 (MQ=255)

                                                                 aTTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTatcat                               >  2:6420461/1‑101 (MQ=255)

                                                                 aTTCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTatcat                               <  2:6420108/101‑1 (MQ=255)

                                                                  ttCTTAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTatcatc                              <  2:6420272/101‑1 (MQ=255)

                                                                     ttAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGt                           >  1:6420466/1‑101 (MQ=255)

                                                                     ttAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAAATCTCTTATCATCAGt                           >  2:6420467/1‑101 (MQ=255)

                                                                      tAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTg                          >  1:6420471/1‑101 (MQ=255)

                                                                      tAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTg                          <  2:6420362/101‑1 (MQ=255)

                                                                      tAATCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTg                          <  2:6419938/101‑1 (MQ=255)

                                                                        aTCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGa                        >  1:6420476/1‑101 (MQ=255)

                                                                        aTCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGa                        <  1:6420264/101‑1 (MQ=255)

                                                                        aTCAATTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGa                        >  2:6420475/1‑101 (MQ=255)

                                                                           aaTTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTAtcatca                             <  2:6419815/93‑1 (MQ=255)

                                                                           aaTTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCg                     >  1:6420482/1‑101 (MQ=255)

                                                                           aaTTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCg                     <  2:6419913/101‑1 (MQ=255)

                                                                           aaTTAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCg                     <  1:6419992/101‑1 (MQ=255)

                                                                              tAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGaa                  <  1:6419799/101‑1 (MQ=255)

                                                                              tAATCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGaa                  >  2:6420486/1‑101 (MQ=255)

                                                                                aTCAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGaaaa                >  1:6420487/1‑101 (MQ=255)

                                                                                  cAGCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAg              >  1:6420491/1‑101 (MQ=255)

                                                                                    gCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTgg                         <  1:6419765/88‑1 (MQ=255)

                                                                                    gCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCa            <  2:6420026/101‑1 (MQ=255)

                                                                                    gCGAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCa            <  2:6419824/101‑1 (MQ=255)

                                                                                      gAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACg          >  1:6420496/1‑101 (MQ=255)

                                                                                      gAAAGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACg          <  2:6420048/101‑1 (MQ=255)

                                                                                       aaaGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGAtt                                                               >  2:6420500/1‑47 (MQ=255)

                                                                                       aaaGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGc         >  1:6420499/1‑101 (MQ=255)

                                                                                         aGCCACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCa       <  1:6419954/101‑1 (MQ=255)

                                                                                           ccACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTg                          >  2:6420505/1‑80 (MQ=255)

                                                                                            cACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCAgcg    <  2:6419785/101‑1 (MQ=255)

                                                                                            cACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCAgcg    >  1:6420506/1‑101 (MQ=255)

                                                                                            cACGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCAgcg    <  1:6419617/101‑1 (MQ=255)

                                                                                             aCGGCAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCAgcgc   >  1:6420509/1‑101 (MQ=255)

TCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCAGCGCA  >  NC_000913/3456933‑3457127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`