BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,929,314	T→C	D97G (GAC→GGC)	viaA ←	protein ViaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,929,314	0	T	C	99.7%	1552.6 / ‑4.6	365	D97G (GAC→GGC)	viaA	protein ViaA
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (186/178); total (186/179)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 368 total aligned reads displayed.

GGAGAAAAAGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCG  >  NC_000913/3929216‑3929411

ggAGAAAAAGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGccc                                                                                                 <  1:7313897/101‑1 (MQ=255)

 gagaAAAAGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCa                                                                                                >  1:7314619/1‑101 (MQ=255)

 gagaAAAAGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCa                                                                                                >  2:7314618/1‑101 (MQ=255)

 gagaAAAAGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCa                                                                                                <  2:7314030/101‑1 (MQ=255)

  agaAAAAGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAg                                                                                               <  1:7314536/101‑1 (MQ=255)

       aaGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGTATCt                                                                                          <  2:7314098/101‑1 (MQ=255)

       aaGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCt                                                                                          >  1:7314625/1‑101 (MQ=255)

       aaGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCt                                                                                          >  2:7314628/1‑101 (MQ=255)

        aGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCtg                                                                                         <  2:7314149/101‑1 (MQ=255)

        aGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCtg                                                                                         >  1:7314630/1‑101 (MQ=255)

          cGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCtgtg                                                                                       <  2:7313922/101‑1 (MQ=255)

          cGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCtgtg                                                                                       >  2:7314632/1‑101 (MQ=255)

           gtgtgtAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTgg                                                                                      <  1:7314354/101‑1 (MQ=255)

                tAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCt                                                                                 <  1:7314092/101‑1 (MQ=255)

                tAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCt                                                                                 >  1:7314639/1‑101 (MQ=255)

                  aCGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGc                                                                               >  1:7314643/1‑101 (MQ=255)

                     cTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGcaca                                                                            <  2:7314088/101‑1 (MQ=255)

                     cTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGcaca                                                                            >  2:7314645/1‑101 (MQ=255)

                      tGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGcaca                                                                            <  1:7314313/100‑1 (MQ=255)

                       gAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAAt                                                                          >  1:7314648/1‑101 (MQ=255)

                        aaGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATa                                                                         <  2:7313959/101‑1 (MQ=255)

                        aaGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATa                                                                         <  2:7313770/101‑1 (MQ=255)

                         aGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATaa                                                                        <  1:7314237/101‑1 (MQ=255)

                          gTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATaaa                                                                       <  1:7314122/101‑1 (MQ=255)

                           tGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAAc                                                                      >  2:7314654/1‑101 (MQ=255)

                            gATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACt                                                                     >  1:7314655/1‑101 (MQ=255)

                            gATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACt                                                                     <  1:7313775/101‑1 (MQ=255)

                            gATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACt                                                                     <  2:7314262/101‑1 (MQ=255)

                            gATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACt                                                                     <  1:7314369/101‑1 (MQ=255)

                             aTCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACtg                                                                    >  2:7314660/1‑101 (MQ=255)

                              tCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACtgt                                                                   <  2:7314192/101‑1 (MQ=255)

                              tCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACtgt                                                                   <  1:7313969/101‑1 (MQ=255)

                              tCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACtgt                                                                   <  1:7314110/101‑1 (MQ=255)

                               cGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACtgtg                                                                  >  2:7314664/1‑101 (MQ=255)

                               cGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACtgtg                                                                  <  2:7314067/101‑1 (MQ=255)

                               cGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACtgtg                                                                  <  1:7314141/101‑1 (MQ=255)

                                gTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTgg                                                                 >  1:7314668/1‑101 (MQ=255)

                                gTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTgg                                                                 <  2:7314143/101‑1 (MQ=255)

                                 tGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGc                                                                <  2:7314168/101‑1 (MQ=255)

                                  gCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCg                                                               >  1:7314672/1‑101 (MQ=255)

                                  gCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCg                                                               >  2:7314673/1‑101 (MQ=255)

                                   cTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGt                                                              <  1:7314169/101‑1 (MQ=255)

                                    tGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTg                                                             >  2:7314678/1‑101 (MQ=255)

                                     gTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTgg                                                            >  1:7314680/1‑101 (MQ=255)

                                      ttAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGga                                                           <  1:7314396/101‑1 (MQ=255)

                                      ttAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGga                                                           >  1:7314685/1‑101 (MQ=255)

                                          cATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAgagg                                                       <  1:7314191/101‑1 (MQ=255)

                                           aTCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAgagga                                                      >  1:7314691/1‑101 (MQ=255)

                                           aTCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAgagga                                                      >  2:7314689/1‑101 (MQ=255)

                                            tCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAgaggag                                                     <  2:7314117/101‑1 (MQ=255)

                                              aaccaacTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCt                                                   >  2:7314699/1‑101 (MQ=255)

                                              aaccaacTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCt                                                   >  1:7314698/1‑101 (MQ=255)

                                               accaacTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTg                                                  <  2:7313901/101‑1 (MQ=255)

                                                 caacTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGc                                                <  1:7314029/101‑1 (MQ=255)

                                                 caacTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGc                                                >  1:7314705/1‑101 (MQ=255)

                                                   acTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAgagg                                                       >  2:7314710/1‑92 (MQ=255)

                                                   acTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCtt                                              <  1:7314146/101‑1 (MQ=255)

                                                   acTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCtt                                              >  1:7314709/1‑101 (MQ=255)

                                                      gTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGc                                           >  1:7314716/1‑101 (MQ=255)

                                                      gTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGT‑AGCCCAGGAt                                                                                            <  2:7314180/51‑1 (MQ=38)

                                                       tCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCt                                          <  1:7314265/101‑1 (MQ=255)

                                                       tCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCt                                          <  2:7314227/101‑1 (MQ=255)

                                                        cGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTg                                         >  2:7314722/1‑101 (MQ=255)

                                                         gggCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCttt                                             >  2:7314728/1‑96 (MQ=255)

                                                         gggCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGa                                        >  1:7314725/1‑101 (MQ=255)

                                                          ggCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTgg                                                                 >  1:7314730/1‑75 (MQ=255)

                                                            cTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAg                                     >  2:7314734/1‑101 (MQ=255)

                                                             ttGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGc                                    >  2:7314739/1‑101 (MQ=255)

                                                              tGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGca                                   >  2:7314744/1‑101 (MQ=255)

                                                               gTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGc                                    >  1:7314747/1‑99 (MQ=255)

                                                                 tCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTg                                                             >  2:7314751/1‑72 (MQ=255)

                                                                 tCTGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACAt                                <  2:7314006/101‑1 (MQ=255)

                                                                   tGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCa                              >  1:7314756/1‑101 (MQ=255)

                                                                   tGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCa                              <  1:7314054/101‑1 (MQ=255)

                                                                   tGCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCa                              >  2:7314755/1‑101 (MQ=255)

                                                                    gCCCATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCAc                             <  1:7314424/101‑1 (MQ=255)

                                                                      ccATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACct                           >  2:7314760/1‑101 (MQ=255)

                                                                       cATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATc                               <  1:7314082/96‑1 (MQ=255)

                                                                       cATGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACctc                          <  1:7314071/101‑1 (MQ=255)

                                                                         tGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCtt                        <  2:7314279/101‑1 (MQ=255)

                                                                         tGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCtt                        <  1:7314378/101‑1 (MQ=255)

                                                                         tGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCtt                        >  1:7314763/1‑101 (MQ=255)

                                                                         tGGAGAATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCtt                        <  1:7314017/101‑1 (MQ=255)

                                                                             gaATTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCgg                     >  2:7314769/1‑100 (MQ=255)

                                                                              aaTTCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTg                   <  2:7314246/101‑1 (MQ=255)

                                                                                 tCAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGt                >  1:7314772/1‑101 (MQ=255)

                                                                                  cAGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGtt               >  1:7314776/1‑101 (MQ=255)

                                                                                   aGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTc              >  1:7314780/1‑101 (MQ=255)

                                                                                   aGACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTc              <  1:7314162/101‑1 (MQ=255)

                                                                                    gACGGTGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTcc             >  2:7314786/1‑101 (MQ=255)

                                                                                    gACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTcc             <  2:7314208/101‑1 (MQ=255)

                                                                                    gACGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTcc             >  1:7314784/1‑101 (MQ=255)

                                                                                      cGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCcgg           >  1:7314790/1‑101 (MQ=255)

                                                                                      cGATGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCcgg           <  1:7314210/101‑1 (MQ=255)

                                                                                         tGCAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCcggcgg        <  2:7314344/101‑1 (MQ=255)

                                                                                           cAGTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGa      <  1:7314287/101‑1 (MQ=255)

                                                                                             gTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACt    <  2:7314392/101‑1 (MQ=255)

                                                                                             gTAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACt    >  2:7314795/1‑101 (MQ=255)

                                                                                              tAAGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTc   >  1:7314799/1‑101 (MQ=255)

                                                                                               aaGCCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCg  >  1:7314803/1‑101 (MQ=255)

GGAGAAAAAGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCG  >  NC_000913/3929216‑3929411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`