BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,144,401	T→C	V136A (GTA→GCA)	pflD →	putative formate acetyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,144,401	0	T	C	100.0%	1414.6 / NA	332	V136A (GTA→GCA)	pflD	putative formate acetyltransferase 2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (155/177); total (155/177)

Only 100 of 332 total aligned reads displayed.

CAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGG  >  NC_000913/4144303‑4144497

cAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCaa                                                                                                >  1:7700902/1‑101 (MQ=255)

 aGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCaaa                                                                                               >  2:7700904/1‑101 (MQ=255)

  gCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCaaaa                                                                                              <  1:7700284/101‑1 (MQ=255)

    gaagaaGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGc                                                                                            <  1:7700190/101‑1 (MQ=255)

    gaagaaGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGc                                                                                            >  2:7700908/1‑101 (MQ=255)

     aagaagATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGcc                                                                                           <  1:7700264/101‑1 (MQ=255)

      agaagaTAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCaaaa                                                                                              <  1:7700343/97‑1 (MQ=255)

      agaagaTAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCcg                                                                                          >  1:7700910/1‑101 (MQ=255)

       gaagaTAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCcgc                                                                                         <  1:7700458/101‑1 (MQ=255)

          gaTAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGAc                                                                                      >  1:7700915/1‑101 (MQ=255)

           aTAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGAcc                                                                                     <  2:7700240/101‑1 (MQ=255)

           aTAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGAcc                                                                                     <  2:7700732/101‑1 (MQ=255)

           aTAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGAcc                                                                                     >  2:7700917/1‑101 (MQ=255)

              aaCGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAac                                                                                  >  2:7700922/1‑101 (MQ=255)

               aCGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAaca                                                                                 <  1:7700411/101‑1 (MQ=255)

                cGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCcg                                                                                          >  2:7700925/1‑91 (MQ=255)

                 gTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACg                                                                               >  1:7700927/1‑101 (MQ=255)

                  tATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGc                                                                              >  1:7700932/1‑101 (MQ=255)

                   aTCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCa                                                                             <  1:7700499/101‑1 (MQ=255)

                    tCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAg                                                                            <  2:7700334/101‑1 (MQ=255)

                    tCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAg                                                                            <  2:7700464/101‑1 (MQ=255)

                       aCCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGAtt                                                                         >  1:7700937/1‑101 (MQ=255)

                        ccGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGAttt                                                                        >  2:7700939/1‑101 (MQ=255)

                         cGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGAtttt                                                                       <  1:7700324/101‑1 (MQ=255)

                          gTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTc                                                                      <  1:7700490/101‑1 (MQ=255)

                          gTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTc                                                                      >  1:7700946/1‑101 (MQ=255)

                          gTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTc                                                                      >  2:7700944/1‑101 (MQ=255)

                          gTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTc                                                                      <  2:7700401/101‑1 (MQ=255)

                           tGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCa                                                                     >  1:7700949/1‑101 (MQ=255)

                             aaGAGTGGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGc                                                                   <  1:7700553/101‑1 (MQ=255)

                                agTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATc                                                                <  2:7700586/101‑1 (MQ=255)

                                agTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATc                                                                <  2:7700636/101‑1 (MQ=255)

                                 gttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCa                                                               >  2:7700954/1‑101 (MQ=255)

                                 gttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCa                                                               <  2:7700505/101‑1 (MQ=255)

                                 gttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCa                                                               <  2:7700573/101‑1 (MQ=255)

                                 gttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCa                                                               <  2:7700685/101‑1 (MQ=255)

                                  ttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCaa                                                              <  2:7700548/101‑1 (MQ=255)

                                   tgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAAc                                                             <  2:7700476/101‑1 (MQ=255)

                                    gttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAAcc                                                            <  2:7700152/101‑1 (MQ=255)

                                     ttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCa                                                           >  2:7700960/1‑101 (MQ=255)

                                      tCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAg                                                          <  1:7700392/101‑1 (MQ=255)

                                        ccGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGAc                                                        <  1:7700547/101‑1 (MQ=255)

                                        ccGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGAc                                                        >  1:7700963/1‑101 (MQ=255)

                                          gTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACgg                                                      <  2:7700313/101‑1 (MQ=255)

                                          gTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACgg                                                      >  2:7700968/1‑101 (MQ=255)

                                          gTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACgg                                                      >  1:7700967/1‑101 (MQ=255)

                                           tACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCa                                                                  <  1:7700370/88‑1 (MQ=255)

                                            aCTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGc                                                                                            >  1:7700975/1‑61 (MQ=255)

                                            aCTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGAt                                                    <  2:7700419/101‑1 (MQ=255)

                                            aCTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGAt                                                    <  1:7700361/101‑1 (MQ=255)

                                             cTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATa                                                   >  2:7700976/1‑101 (MQ=255)

                                              tGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATaa                                                  >  1:7700981/1‑101 (MQ=255)

                                               gggAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATaaa                                                 >  1:7700985/1‑101 (MQ=255)

                                                 gAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAgg                                               >  1:7700989/1‑101 (MQ=255)

                                                  aaaaaCGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGc                                              >  2:7700990/1‑101 (MQ=255)

                                                   aaaaCGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGcc                                             <  2:7700655/101‑1 (MQ=255)

                                                    aaaCGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCa                                            <  1:7700513/101‑1 (MQ=255)

                                                    aaaCGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCa                                            <  2:7700430/101‑1 (MQ=255)

                                                     aaCGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAg                                           <  2:7700564/101‑1 (MQ=255)

                                                     aaCGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAg                                           <  1:7700522/101‑1 (MQ=255)

                                                      aCGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAgg                                          >  2:7700995/1‑101 (MQ=255)

                                                       cGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAggg                                         <  1:7700436/101‑1 (MQ=255)

                                                        gTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAgggg                                        >  1:7700998/1‑101 (MQ=255)

                                                        gTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAgggg                                        <  1:7700222/101‑1 (MQ=255)

                                                         ttCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGc                                       <  2:7700379/101‑1 (MQ=255)

                                                          tCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATaaa                                                 <  2:7700078/90‑1 (MQ=255)

                                                          tCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGca                                      <  1:7700579/101‑1 (MQ=255)

                                                           cGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGcac                                     <  2:7700690/101‑1 (MQ=255)

                                                            gATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCa                                                                             <  2:7700414/60‑1 (MQ=255)

                                                              tGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACatt                                  <  1:7700204/101‑1 (MQ=255)

                                                              tGAAAGATTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACatt                                  >  1:7701002/1‑101 (MQ=255)

                                                              tGAAAGATTCCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACatt                                  >  2:7701003/1‑101 (MQ=255)

                                                                    aTTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACattattatt                            <  1:7700543/101‑1 (MQ=255)

                                                                    aTTTCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACattattatt                            >  1:7701009/1‑101 (MQ=255)

                                                                     tttCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTg                           >  2:7701010/1‑101 (MQ=255)

                                                                     tttCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTg                           <  1:7700534/101‑1 (MQ=255)

                                                                      ttCATCAACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGa                          >  2:7701016/1‑101 (MQ=255)

                                                                       tcatcaACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGAt                         <  1:7700164/101‑1 (MQ=255)

                                                                       tcatcaACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGAt                         >  1:7701017/1‑101 (MQ=255)

                                                                         atcaACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTa                       <  2:7700529/101‑1 (MQ=255)

                                                                         atcaACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTa                       >  1:7701022/1‑101 (MQ=255)

                                                                          tcaACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTAc                      >  2:7701023/1‑101 (MQ=255)

                                                                           caACGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTAcc                     <  1:7700765/101‑1 (MQ=255)

                                                                            aaCGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTAccc                    >  1:7701029/1‑101 (MQ=255)

                                                                              cGGGCAGATGACAGATGAAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCAc                  >  2:7701032/1‑101 (MQ=255)

                                                                                  cagatgacagatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCt              <  2:7700598/101‑1 (MQ=255)

                                                                                  cagatgacagatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCt              <  2:7700442/101‑1 (MQ=255)

                                                                                   agatgacagatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAgg                                          >  2:7701038/1‑72 (MQ=255)

                                                                                   agatgacagatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTg             >  1:7701036/1‑101 (MQ=255)

                                                                                      tgacagatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCa                   >  2:7701043/1‑92 (MQ=255)

                                                                                      tgacagatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTg          >  1:7701042/1‑101 (MQ=255)

                                                                                          agatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATc      <  1:7700691/101‑1 (MQ=255)

                                                                                          agatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATc      >  2:7701048/1‑101 (MQ=255)

                                                                                          agatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATc      >  2:7701052/1‑101 (MQ=255)

                                                                                           gatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCa     <  2:7700517/101‑1 (MQ=255)

                                                                                           gatgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCa     >  1:7701054/1‑101 (MQ=255)

                                                                                            atgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCAc    <  1:7700612/101‑1 (MQ=255)

                                                                                            atgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCAc    >  2:7701056/1‑101 (MQ=255)

                                                                                             tgaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACg   <  2:7700789/101‑1 (MQ=255)

                                                                                              gaAGCAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACgg  <  1:7700955/101‑1 (MQ=255)

CAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGG  >  NC_000913/4144303‑4144497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`