| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 514,159 | 0 | T | G | 60.0% | ‑0.6 / 13.9 | 15 | V56G (GTG→GGG) | cueR | DNA‑binding transcriptional dual regulator CueR |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (9/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGC > NC_000913/514094‑514223 | cGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGgggg > 1:2178652/1‑68 (MQ=255) gTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGc < 1:137511/68‑1 (MQ=255) cGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACTGCCCCAGGCACGGCAGGGGGGCTTTaa > 1:3448001/1‑68 (MQ=255) gCACCTACACGCCGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAAc < 1:2983510/68‑1 (MQ=255) cACCTACACGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGGGGGCTTTAAcc > 1:1074223/1‑68 (MQ=255) acGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGGGGGGTTTTACCTGGAaga > 1:2932645/1‑68 (MQ=255) acGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGGGGGGTTTAACCTGGAaga > 1:1118532/1‑68 (MQ=255) acGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGGGGGGTTTAAACTGGAagc > 1:2227577/1‑67 (MQ=255) gcagcagcaTCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGGGGGCTTTTAACTGGAagagg > 1:2285229/1‑67 (MQ=255) gcagcagcaTCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGGGGGCTTTAACCTGGAagaga > 1:453653/1‑68 (MQ=255) aTCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGGGGGGTTTAACCTGGGAGAGAGGGGCGAg > 1:3360614/1‑68 (MQ=255) aTCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGGGGGCTTTAACCTGGAAGAGAGCGGCGAg > 1:1036163/1‑68 (MQ=255) ctcAACGAACTGACCTTACTGCGCCAGGCACGGCAGGGGGGCTTTAACCTGGAAGCGAGCGGCGAGCt > 1:2984341/1‑68 (MQ=255) ctcAACGAACTGACCTTACTGCGCCAGGCACGGCAGGGGGGCTTTAACCTGGAAGAGAGCGGCGAGCt > 1:2432844/1‑68 (MQ=255) gAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGaa < 1:3246487/68‑1 (MQ=255) aCTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATc < 1:950663/68‑1 (MQ=255) gACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGt < 1:277926/68‑1 (MQ=255) ccTTACTGCGCCAGGCACGGCAGGTGGGGTTTAACCTGGAAGAGAGGGGCGAGGTGGGGAATCTGttt > 1:2293987/1‑68 (MQ=255) tACTGCGCCAGGCACGGCAGGGGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGGGAATCTGTTTAAc > 1:1974830/1‑68 (MQ=255) cAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCa < 1:189703/68‑1 (MQ=255) cAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCa < 1:596502/68‑1 (MQ=255) aCGGCAGGGGGGCTTGGACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGc < 1:707648/68‑1 (MQ=255) aGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAgc < 1:1111901/68‑1 (MQ=255) | CGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGC > NC_000913/514094‑514223 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |