| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 821,440 | 0 | C | T | 31.6% | 53.0 / 17.3 | 38 | F216F (TTC→TTT) | ybhM | Bax1‑I family protein YbhM |
| Reads supporting (aligned to +/- strand): ref base C (4/22); new base T (12/0); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGT > NC_000913/821374‑821494 | gTTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGcttct < 1:2080734/68‑1 (MQ=255) tgtgGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCttt < 1:1643921/68‑1 (MQ=255) tgGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTgg < 1:2644188/68‑1 (MQ=255) ggAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTggg < 1:2528534/68‑1 (MQ=255) ggAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCCTTTTTtgg > 1:1417198/1‑68 (MQ=255) aGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTTTTTtgggt > 1:3354305/1‑66 (MQ=255) aGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTTTTTTGGAt > 1:1412825/1‑68 (MQ=255) aGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTTTTTGGGAt > 1:352100/1‑68 (MQ=255) aGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTtgggt > 1:1389733/1‑66 (MQ=255) aGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATAGGCTTTTTTtgggt > 1:3017448/1‑66 (MQ=255) ccGGTTCGTTGCGCTTTTACAGGTGCATTAAGACTCTATTACTATTTTATCGGCTTTTTTTGGGTACt > 1:1935788/1‑68 (MQ=255) ccGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTTTTTTTGATACt > 1:2945681/1‑68 (MQ=255) ccGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACt < 1:2616021/68‑1 (MQ=255) ccGGTTCCTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACt < 1:2952101/68‑1 (MQ=255) ggTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCAGCTTCTTTGGGATACTTg < 1:2175259/68‑1 (MQ=255) gTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCcgcg < 1:1492341/68‑1 (MQ=255) gcTTTTACAGGTGCATTAACGCTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGc < 1:1747217/68‑1 (MQ=255) gcTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGc < 1:715747/68‑1 (MQ=255) gcTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGc < 1:1417730/68‑1 (MQ=255) cTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCt < 1:266521/68‑1 (MQ=255) cTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCt < 1:89457/68‑1 (MQ=255) cTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCt < 1:2353378/68‑1 (MQ=255) aCAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAAc < 1:834362/68‑1 (MQ=255) cAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATGCTTGCCGCGATAGCTATAACg < 1:1477132/68‑1 (MQ=255) cAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACg < 1:3192759/68‑1 (MQ=255) aGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTTGGGTACTTGCCCCGCTCGCTATAACGc > 1:2210028/1‑68 (MQ=255) aTTAACACTCTATTACTATTTTATCGGCTTCCTTGGGATAACTGTCGCGATTGCTATAAAGCTTGGCt > 1:1650907/1‑68 (MQ=255) tAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTgg < 1:1979191/68‑1 (MQ=255) cacTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCaa < 1:1339084/68‑1 (MQ=255) acTCTATTACTATTTTATCGGCTTTTTTTGGGTACTTTCCGCGGTAGCTATAACGCTTGTCTGGGaaa > 1:1896485/1‑68 (MQ=255) acTCTATTACTATTTTATCGGCTTTTTTTGGATACTTTCCGCGAGAGCTATAACGCTTGTCTGGCaaa > 1:2867892/1‑68 (MQ=255) acTCTATTACTATTTTATCGGCTTCTTTTGGGTTCTTGTCGCGGTAGCTATAACGCTTGTCTGGGaaa > 1:688561/1‑68 (MQ=255) acTCTANTACTATTTTATCGGCTTTTTTTGGATACTTGCCGCGATAGCTATAAAGCTTGTCTGGCaaa > 1:2029583/1‑68 (MQ=255) tctATTACTATTTTATCGGCTTTTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAgg > 1:268590/1‑68 (MQ=255) tctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAgg < 1:401674/68‑1 (MQ=255) ctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGc < 1:2799809/68‑1 (MQ=255) ctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGc < 1:3059496/68‑1 (MQ=255) ctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGc < 1:88615/68‑1 (MQ=255) ctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGc < 1:1185557/68‑1 (MQ=255) aTTACTATTTTATCGGCTTCTTTTGGATAATTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCAt > 1:2840591/1‑68 (MQ=255) tATTTTATCGGCTTTTTTTGGGTACTTTCCCCGACAGGTATAACACTTTTCTGGCAAAAGGAAAcgcg > 1:2017991/1‑68 (MQ=255) tATTTTATCGGCTTTTTTTGGGTACTTGCCCCGATAGGTATAAACCTTGTCTGGCCAAGGCAAAagcg > 1:2749405/1‑68 (MQ=255) tATTTTATCGGCTTTTTTTGGGTACTTGCCCCGATAGCTATAACGCTTGTCTGGCAAAGGCATAcgcg > 1:1809670/1‑68 (MQ=255) caTTTTATCGGCTTCTTTTGGATACTTTCCGCGATAGCTATAACCCTTGTCTGGCAAAGGCATAcgcg > 1:2736264/2‑68 (MQ=255) aTTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGt < 1:991922/68‑1 (MQ=255) | GTTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGT > NC_000913/821374‑821494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |