| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,223,410 | 0 | T | G | 38.5% | 32.8 / 13.0 | 26 | V409G (GTG→GGG) | dld | D‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (3/13); new base G (10/0); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTT > NC_000913/2223346‑2223476 | tACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtgggggg > 1:1869821/1‑67 (MQ=255)tACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtgggggg > 1:3203132/1‑67 (MQ=255)tACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtgggggg > 1:1373479/1‑67 (MQ=255)tACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAAACGTGGCtgggggg > 1:1790188/1‑67 (MQ=255)tACGAGCATCATCTGCTGTTAAAAACGGCGGGCGAGGGCGTGGGCGACGCCCAATCGTGGCtgggggg > 1:2658091/1‑67 (MQ=255) cGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGgtt > 1:401059/1‑68 (MQ=255) atcatcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTTTTTc > 1:1248444/1‑68 (MQ=255) catcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCaa < 1:1350094/68‑1 (MQ=255) tcTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTTAAAc > 1:1694022/1‑68 (MQ=255) tgTTAAAAATGGCGGGCGGGGGTGTGGGGGAAGCCGAAAAGGGGGTGGGGGGTTATTTCAAACAggac > 1:1649003/1‑66 (MQ=255) aaaaGGGCGGCGCATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAgg < 1:2142439/68‑1 (MQ=255) aTGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTTCAACAGGCCGAAggggg > 1:810349/1‑65 (MQ=255) gcgggcgATGGCTTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGAttt < 1:3072998/68‑1 (MQ=255) gcgggcgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGAttt < 1:2762837/68‑1 (MQ=255) cgATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTg < 1:965474/68‑1 (MQ=255) tGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCt < 1:1455909/68‑1 (MQ=255) tGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCt < 1:25365/68‑1 (MQ=255) tGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCt < 1:2118746/68‑1 (MQ=255) ggCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTg > 1:550630/1‑68 (MQ=255) gCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGt < 1:1259371/68‑1 (MQ=255) cGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTa > 1:218910/1‑68 (MQ=255) cGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTa > 1:10995/1‑68 (MQ=255) gCGAAGCCAAATCGTGGCTGGTGGATTATTTCCAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCg > 1:1446888/1‑68 (MQ=255) gCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGGTTTCTTTGTCTGTACGCCg > 1:1174819/1‑68 (MQ=255) gCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGCAGGCGACTTCTTTGTCTGTACGCCg > 1:2309083/1‑68 (MQ=255) gCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGGGATTTCTTTGTCTGTACGCCg > 1:3445730/1‑68 (MQ=255) gCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCg > 1:2530152/1‑68 (MQ=255) gCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAAAAGGCCGAAGGCGATTTCTTTGTCTTTACGCCg > 1:1107233/1‑68 (MQ=255) gCGAAGCCAAATCGTGGCTGGAGGATTATTTCAAACAGGCCGGAGGCGATTTCTTTTTCTGTATGCCg > 1:1512214/1‑68 (MQ=255) ccAAATCGTGGCTGGTGGGTTTTTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCggaggct > 1:1342980/1‑66 (MQ=255) ccAAATCGTGGCTGGTGGGTTATTTTAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGaa > 1:1007523/1‑68 (MQ=255) ccAAATCGTGGCTGGGGGATTATTTCAAAAAGGCCGAAGGCGATTTCTTTGTCTGTACGCCggaggga > 1:2432592/1‑66 (MQ=255) cAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAg < 1:770747/68‑1 (MQ=255) cAAATCGTGGCTGGTGGAGTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAg < 1:3270361/68‑1 (MQ=255) aTCGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGGGGTTTCTTTGTCTGTACGCCGGGGGAAGgca > 1:1084353/1‑68 (MQ=255) tCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGgcag < 1:2429006/68‑1 (MQ=255) cGTGGCTGGGGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGGAGgcagc > 1:2358232/1‑68 (MQ=255) ggCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCaaa < 1:740035/68‑1 (MQ=255) gtggATTATTTCAACCAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGtt < 1:2025566/68‑1 (MQ=255) gtggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGtt < 1:1016484/68‑1 (MQ=255) gtggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGtt < 1:3133024/68‑1 (MQ=255) gtggATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGtt < 1:227776/68‑1 (MQ=255) gtggATTATTGCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGtt < 1:2956022/68‑1 (MQ=255) | TACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTCTGTACGCCGGAGGAAGGCAGCAAAGCGTT > NC_000913/2223346‑2223476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |