| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,302,928 | 0 | T | G | 35.5% | 47.0 / 13.4 | 31 | V147G (GTG→GGG) | yhbV | putative peptidase YhbV |
| Reads supporting (aligned to +/- strand): ref base T (0/20); new base G (11/0); total (11/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATATTGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGC > NC_000913/3302863‑3302992 | aaTATTGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGa < 1:1409337/68‑1 (MQ=255) ttGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCt > 1:1205481/1‑68 (MQ=255) tGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTg < 1:2867112/68‑1 (MQ=255) tGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTg < 1:1504250/68‑1 (MQ=255) tGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTg < 1:15177/68‑1 (MQ=255) tGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTg < 1:82384/68‑1 (MQ=255) tGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTg < 1:1836455/68‑1 (MQ=255) tGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTg < 1:2616056/68‑1 (MQ=255) tGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTg < 1:485232/68‑1 (MQ=255) cTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCt < 1:3339910/68‑1 (MQ=255) aCAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATc < 1:2292133/68‑1 (MQ=255) ggCATANTGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGCATGGGGCATCTGCTTAATCAggg > 1:2030616/1‑66 (MQ=255) atgCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGGCTGGCTGGTGAATCTGCTTAATCAGTGCGATGa < 1:2453084/68‑1 (MQ=255) atgCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTTCTTAATCAGTGCGATGa > 1:764113/1‑68 (MQ=255) atgCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGGTGa > 1:3003067/1‑68 (MQ=255) atgCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGATGa > 1:812075/1‑68 (MQ=255) atgCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAAACTTCTTAATCAGTGCGCTgg > 1:2643863/1‑67 (MQ=255) tgCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAg < 1:1129486/68‑1 (MQ=255) tgtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAAATTGCCTAATCAGTGGGATGAGCGGGGCAt > 1:3139837/1‑68 (MQ=255) gtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCAtt < 1:2218/68‑1 (MQ=255) gtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCAtt < 1:2755716/68‑1 (MQ=255) gtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGATGAGCCGGGCAtt > 1:770298/1‑68 (MQ=255) catccAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGTCATTCg < 1:2076478/65‑1 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGGGGATCTTCTTAATTAGTGGGATGAGCTGGGCATTTGTa > 1:2971831/1‑68 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATTTGCTTAATCAGTGCGATGAGGTGGGCGTTCGTa > 1:1097047/1‑68 (MQ=38) gCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTTATTATTGCGATGAGCTGGGGATTCgga > 1:966509/1‑66 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGATGAGCCGGGCATTCGTa > 1:401055/1‑68 (MQ=255) ccAGTGGAGCTTTCCCGCGCCTGGCTGGGGAATCTGCTTAATCAGTGCGCTGAGGCGGGGATTCGTaa > 1:1406821/1‑68 (MQ=255) ccAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGGTGAGCTGGGCATTCGTaa > 1:785702/1‑68 (MQ=255) ccAGTGGAGCTTTCCCGCGACTGGCTGGGGGATCTGCTTAATCCGTGCGATGAGCTGGGCATTTGTaa > 1:2759698/1‑68 (MQ=255) ccAGTGGAGCTTTCCCGCGACTGGCTGGGGGATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTaa > 1:2619919/1‑68 (MQ=255) ccAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGTGACGAGCTGGGCATTCGTaa > 1:344894/1‑68 (MQ=255) ccAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTaa > 1:35125/1‑68 (MQ=255) ccAGTGGAGCTATCCCGCNACTGGCTGGGGAATCTGCTTTATCAGTGCGATGAGCTGGGCATTCGTaa > 1:2573018/1‑68 (MQ=38) tGGAGCTTTCCCGCGACTGGCTGGTGAATTTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAg > 1:3383726/1‑68 (MQ=255) tGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAg > 1:1618425/1‑68 (MQ=255) tGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATAAGTGCGATGGGCTGGGCATTCGTAACCAg > 1:170668/1‑68 (MQ=255) ggAGCTTTCCCGCGACTGGCTGGTGAATCTGGTTAATCAGTGCGAAGGGCCGGGCATTCGTCACCAGt > 1:1733400/1‑68 (MQ=255) tttCCCGCGACTGGCTGGGGAATCTGCTTAATTAGTGCGGTGAGGTGGGGATTTGTAAACAGTTTGaa > 1:2219350/1‑68 (MQ=255) gACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGt < 1:3199954/68‑1 (MQ=255) gACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGt < 1:1651692/68‑1 (MQ=255) gACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGt < 1:1611313/68‑1 (MQ=255) gctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTgg < 1:2429506/68‑2 (MQ=255) gctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGa < 1:2587874/68‑1 (MQ=255) gctggTGAATCTGCTTAATCAGTGCGATGAGCCGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGa < 1:2655867/68‑1 (MQ=255) tggTGAATCTGCTTAATCAGTGCGATGAGCTGTGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGc < 1:2659220/68‑1 (MQ=255) | AATATTGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGC > NC_000913/3302863‑3302992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |