| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,563,481 | 0 | T | G | 30.6% | 51.4 / 27.2 | 36 | V490G (GTG→GGG) | glpD | aerobic glycerol 3‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/25); new base G (11/0); total (11/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.66e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGAT > NC_000913/3563416‑3563544 | gCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggtgg < 1:2854153/68‑1 (MQ=255)gCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggtgg < 1:2259013/68‑1 (MQ=255) cGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGa < 1:3275149/68‑1 (MQ=255) gTCGCANAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggggg > 1:2029796/1‑66 (MQ=255) gTCGCACAAGACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 1:2961642/68‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggggg > 1:870940/1‑66 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggggg > 1:2947206/1‑66 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 1:996744/68‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 1:2335659/68‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 1:2052370/68‑1 (MQ=255) gCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat < 1:1185670/68‑1 (MQ=255) aaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCGGTGGCTGGTGGAGTAT‑ACgca < 1:2477479/68‑1 (MQ=255) aaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACgca < 1:2549853/68‑1 (MQ=255) gacaGGGATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACgcagc < 1:508567/65‑1 (MQ=255) gacaGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACgcagc < 1:1689504/65‑1 (MQ=255) aCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTAAGTCAGTGGCTGGGGGAGTAT‑ACgccac > 1:2960392/1‑65 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAgg < 1:1280864/68‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAgg < 1:3178202/68‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGAT‑AAGCAGCAGAgg > 1:431989/1‑68 (MQ=255) gCAGGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAgg < 1:2773606/68‑1 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGt < 1:2563754/68‑1 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGAT‑AAGCGGCAGAggg > 1:3058669/1‑67 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGAT‑AAGCCGCAGAggg > 1:2590025/1‑67 (MQ=255) tgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATc < 1:687383/68‑1 (MQ=255) gCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGAT‑ACGCAGCCGAGGGGATCGc > 1:1762201/1‑68 (MQ=255) aaTGCGGATCAACAATCTCGCGTGAGTCAGTGGCTGGGGGGGTAA‑AACCAGCACCAGGTATAGCTGGc > 1:494792/1‑68 (MQ=255) tGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGAT‑ACGCAGCAGAGGGTATTGCTggggg > 1:812488/1‑65 (MQ=255) gCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATAAGGCGGCGAAGGTTTTCCCTGGcgt > 1:3134344/1‑68 (MQ=255) gCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGAT‑ACGCCGCAGAGGGTATTGCTGGcggg > 1:2243861/1‑66 (MQ=255) gCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGAT‑AAGCAGCAGAGGGTATTGCCGGggtg > 1:1786786/1‑67 (MQ=255) gATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGAT‑ACGCAGCAGAGGGTATCGCTGgcggcgga > 1:147546/1‑66 (MQ=255) caaTCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTaa < 1:2427182/68‑1 (MQ=255) caaTCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTaa < 1:2336989/68‑1 (MQ=255) aaTCTCGTGTGAGTCAGTGGCTGGGGGGGGAT‑ACGCAGCAGAGGGTATCGCTGGCGGCGGTAATTaaa > 1:1319059/1‑67 (MQ=255) ctcGTGTGAGTCAGTGGCTGGGGGGGGAT‑ACGCAGCAGAGGGTATCGCTGGGGGCGTAAAATAAAGTa > 1:1727269/1‑68 (MQ=255) tcGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTaa < 1:2603260/68‑1 (MQ=255) tcGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTaa < 1:3232363/68‑1 (MQ=255) cGTGTGAGTCAGTGGCTGGGGGGGGAT‑AAGCAGCAGAGGGTATCGCTTGGGGCGGAAATTaaagaaag > 1:3305001/1‑68 (MQ=255) cGTGTGAGTCAGTGGCTGGGGGGGGAT‑AAGCAGCAGAGGGTATCGCTGGGGGCGTAAATTAAAGTAAg > 1:1471683/1‑68 (MQ=255) gtgtGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAgg < 1:2594010/68‑1 (MQ=255) tgtgAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGt < 1:1081104/68‑1 (MQ=255) tgtgAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGAGTCGTAAATTAACGTAAGGt < 1:1965496/68‑1 (MQ=255) cAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAGTTAACGTAAGGTGATCAgg < 1:54610/68‑1 (MQ=255) cAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAgg < 1:596891/68‑1 (MQ=255) gCTGGTGGAGTAT‑GCGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGa < 1:157422/68‑1 (MQ=255) gCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGa < 1:3161723/68‑1 (MQ=255) gCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGa < 1:815666/68‑1 (MQ=255) cTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTGAGAt < 1:1728453/68‑1 (MQ=255) cTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGAt < 1:409638/68‑1 (MQ=255) | GCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTAT‑ACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGAT > NC_000913/3563416‑3563544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |