| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,020,316 | 0 | A | C | 35.0% | 24.4 / 11.5 | 20 | T31P (ACC→CCC) | ubiB | ubiquinone biosynthesis protein UbiB |
| Reads supporting (aligned to +/- strand): ref base A (13/0); new base C (0/7); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTATATTTCATCATTCGCACTTTTTTAAGCTACGGACTTGATGAACTGATCCCCAAAATGCGTATCACCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGCATAAAGACAAACTTTT > NC_000913/4020249‑4020383 | ccTATATTTCATCATTCGCACTTTTTTAAGCTACGGACTTGATGAACTGATCCCCAAAATGCGTATCa < 1:1108603/68‑1 (MQ=255) atTTCATCATTCGCACTTTTTTAAGCTACGGACTTGATGAACTGATCCCCAAAATGCGTATCACCCTg > 1:1899275/1‑68 (MQ=255) ttCATCATTCGCACTTTTTTAAGCTACGGACTTGATGAACTGATCCCCAAAATGCGTATCACCCTGcc > 1:1500393/1‑68 (MQ=255) tcatcaCTCGCACTTTTTTAAGCTACGGACTTGATGAACTGATCCCCAAAATGCGTATCACCCTGCCg > 1:42823/1‑68 (MQ=255) tCGCATTTTTTTAAGCTACGGACTTGATGAACTGATCCCAAAAATGCGTATCCCCCTGCCGCTACGGc < 1:916301/68‑1 (MQ=255) tCGCACTTTTTTAAGCTACGGACTTGATGAACGGATCCCCAAAATGCGTATCCCCCTGCCGCTACGGc < 1:1175502/68‑1 (MQ=255) ccTTTTTTAAGCTACGGACTTGATGAACTGATCCCCAAAATGCGTATCCCCCTGCCGCTACGGCTATg < 1:1042784/67‑1 (MQ=255) taggCTACGGACTTGATGAACTGATCCCAAAAATGCGTATCCCCCTGCCGCTACGGCTATGGCGATAc < 1:802314/65‑1 (MQ=255) gCTACGGACTTGATGAACTGATCCCCAAAATGCGTATCACCCTGCCGCTACGGCTATGGCGATACTCa > 1:855944/1‑68 (MQ=255) gCTACGGACTTGATGAACTGATCCCCAAAATGCGTATCACCCTGCCGCTACGGCTAAGGCTATACTCa > 1:273725/1‑68 (MQ=255) gCTACGGACTTGATGAACTGATCCCCAAAATGCGTACCCCCCTGCCGCTACGGCTATGGCGATACTCa < 1:3444943/68‑1 (MQ=255) gCTACGGACTTGATGAACGGATCCCCAAAAGGCGTACCACCCTGCCGCTACGGCTATGGCGATACTCa < 1:2917915/68‑1 (MQ=255) gCTACGGACTTGATGAACGGACCCCCAAAATGCGTATCCCCCTGCCGCTACGGCTATGGCGATACTCa < 1:2565233/68‑1 (MQ=255) cGGACTTGATGAACTTACCCCCAAAATGCGTATCCCCCTGCCGCTACGGCTATGGCGATACTCattat < 1:94998/68‑1 (MQ=255) cGGACTTGATGAACTGATCCCAAAAATGCGTATCCCCCTGCCGCTACGGCTATGGCGATACTCattat < 1:1498595/68‑1 (MQ=255) aCTTGATGAACTGATCCCCAAAATGCGTATCACCCTGCCGCTACGGCTATGGCGATACTCATTATTCt > 1:1473070/1‑68 (MQ=255) cTTGATGAACTGATCCCCAAAATGCGTATCACCCTGCCGCTACGGCTATGGCGATACTCATTATTCTg > 1:2745105/1‑68 (MQ=255) tgatgaACTAACCCCCAAAATGCTTTCCCCCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGa < 1:2835724/68‑1 (MQ=255) tgaACTGATCCCCAAAATGCGTATCACCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGc > 1:800179/1‑68 (MQ=255) aaCTGATCCCCAAAATGCGTATCACCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCa > 1:3291415/1‑68 (MQ=255) aaCTGATCCCCAAAATGCGTATCACCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCa > 1:1447813/1‑68 (MQ=255) ccAAAATGCGTATCACCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGCAt > 1:1642493/1‑68 (MQ=255) aaaaTGCGTATCACCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGCATaa > 1:1892577/1‑68 (MQ=255) tATCCCCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGCATAAAGACGAAc < 1:582200/68‑1 (MQ=255) tATCCCCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGCATAAAGACAAAc < 1:1657081/68‑1 (MQ=255) tATCCCCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGCATAAAGACAAAc < 1:155428/68‑1 (MQ=255) tATCCCCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGCATAAAGACAAAc < 1:1487317/68‑1 (MQ=255) tATCCCCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGCATAAAGACAAAc < 1:695136/68‑1 (MQ=255) tCACCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGCTGCCAAATCGGCATAAAGACAACCtt > 1:2976107/1‑68 (MQ=255) aCCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGGCGAAAGACAAACtttt > 1:1310988/1‑68 (MQ=255) aCCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGCATAAAGACAAACtttt > 1:1738087/1‑68 (MQ=255) | CCTATATTTCATCATTCGCACTTTTTTAAGCTACGGACTTGATGAACTGATCCCCAAAATGCGTATCACCCTGCCGCTACGGCTATGGCGATACTCATTATTCTGGATGCCAAATCGGCATAAAGACAAACTTTT > NC_000913/4020249‑4020383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |