| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 44.0% | 35.1 / 99.3 | 75 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base C (23/19); new base T (12/21); total (35/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC > NC_000913/4295994‑4296125 | cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg < 1:668997/68‑1 (MQ=35)cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg < 1:340800/68‑1 (MQ=35) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc > 1:668942/1‑68 (MQ=25) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc < 1:1536261/68‑1 (MQ=35) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc > 1:1683074/1‑68 (MQ=35) aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTg > 1:2303618/1‑68 (MQ=25) aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTg > 1:2191049/1‑68 (MQ=25) aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg > 1:2601803/1‑68 (MQ=35) aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg > 1:1884705/1‑68 (MQ=35) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa < 1:726366/68‑1 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATGAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt < 1:2286598/68‑1 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt > 1:3029701/1‑68 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt > 1:2676891/1‑68 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt > 1:2499649/1‑68 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt < 1:3399017/68‑1 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt > 1:1786457/1‑68 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt > 1:645599/1‑68 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt > 1:1342366/1‑68 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt > 1:1922456/1‑68 (MQ=35) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt > 1:3330386/1‑68 (MQ=35) aGCCTGATTTAGAGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt > 1:2128715/1‑68 (MQ=255) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc > 1:2487770/1‑68 (MQ=35) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCg > 1:1215689/1‑68 (MQ=35) tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGa < 1:3320500/68‑1 (MQ=25) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc < 1:1403667/68‑1 (MQ=35) aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACg < 1:3125562/68‑1 (MQ=25) aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg < 1:2184412/68‑1 (MQ=35) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCt < 1:2672109/68‑1 (MQ=25) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt > 1:435734/1‑68 (MQ=35) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt > 1:2852414/1‑68 (MQ=35) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt > 1:2429603/1‑68 (MQ=35) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt > 1:1525440/1‑68 (MQ=35) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCAGATGCGACGCt > 1:2694579/1‑68 (MQ=25) aGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg < 1:3082095/68‑1 (MQ=255) aGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTg < 1:3247712/68‑1 (MQ=37) cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc > 1:1591498/1‑68 (MQ=255) gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg < 1:661868/68‑1 (MQ=255) gATTTTGTAGGGCGGATAAGGCGTTTGCGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgcg < 1:652252/68‑1 (MQ=14) tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa < 1:1113843/68‑1 (MQ=255) aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa > 1:505078/1‑68 (MQ=255) gtgggATAGGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:1911071/65‑1 (MQ=14) gTCGTATAGGGCATTTCCGCCCCATAAGACGTAAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:2418971/68‑1 (MQ=9) gTCGGATAAGGCTTTTACGCCGCGTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:2473315/68‑1 (MQ=14) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:4209/68‑1 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:3274267/68‑1 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:408857/68‑1 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:434148/68‑1 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:635097/68‑1 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:2918239/68‑1 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:1883966/68‑1 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg > 1:3124689/1‑68 (MQ=255) acaggATAAGCCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:845125/65‑1 (MQ=255) aTAAGGGATTTCCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:1950087/68‑1 (MQ=14) aTAAGGCGTTTGCGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:629178/68‑2 (MQ=14) aTAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:1009583/68‑2 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:821034/68‑2 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:1174596/68‑2 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:1706529/68‑2 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:1963377/68‑2 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:2728361/68‑1 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:217136/68‑1 (MQ=255) aTAAGGCGATTACGCGGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:237028/68‑2 (MQ=14) aTAAGGCGATTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:2521195/68‑2 (MQ=14) tAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCNCGTCTTATCAGGCCTatc < 1:2572111/68‑3 (MQ=14) aaGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct > 1:1817892/1‑65 (MQ=255) ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa > 1:741836/1‑68 (MQ=255) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACTCCAGa > 1:144718/1‑68 (MQ=255) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa > 1:3272181/1‑68 (MQ=255) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa > 1:265992/1‑68 (MQ=255) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCGAccccggc > 1:726006/1‑65 (MQ=14) tttCCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa < 1:1770050/68‑1 (MQ=255) tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa < 1:2832746/68‑1 (MQ=255) tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGActgt > 1:3271271/1‑65 (MQ=255) gccgccgcATCCGACATAAACGCGTGATGCGACGATTGCCTCGTCTTTTCAGGCCTACGCCAGACAgc < 1:3412787/66‑1 (MQ=14) aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg > 1:701122/1‑68 (MQ=255) aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg > 1:1340680/1‑68 (MQ=255) aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg > 1:334803/1‑68 (MQ=255) cgccgcATCCGACATCGCCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc < 1:2942073/68‑1 (MQ=255) aTCGGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 1:1783423/68‑1 (MQ=255) aTCCGACATCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 1:1785392/68‑1 (MQ=255) aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 1:14270/68‑1 (MQ=40) aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 1:3379545/68‑1 (MQ=40) aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 1:3045000/68‑1 (MQ=40) aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 1:2215542/68‑1 (MQ=40) cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtt > 1:3143094/1‑68 (MQ=40) tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa > 1:1524285/1‑68 (MQ=39) aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc < 1:1921615/68‑1 (MQ=35) | CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGC > NC_000913/4295994‑4296125 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |