| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,414,906 | 0 | T | G | 37.5% | 24.6 / 19.7 | 24 | V211G (GTG→GGG) | aidB | putative acyl‑CoA dehydrogenase AidB |
| Reads supporting (aligned to +/- strand): ref base T (0/15); new base G (9/0); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGT > NC_000913/4414843‑4414973 | gTTGCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTgggg < 1:2618831/68‑1 (MQ=255) tCCGATGGTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑Ggggggggg > 1:1866201/1‑66 (MQ=255) ccGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCAt < 1:1540439/68‑1 (MQ=255) tATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGt < 1:1134268/68‑1 (MQ=255) gCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGGGGGGGGACAATTGGttttt > 1:3330425/1‑68 (MQ=255) gCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGGTAAATGGgttttt > 1:1795626/1‑67 (MQ=255) gCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGGTAAATGGgttttt > 1:1071703/1‑67 (MQ=255) accaccCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGt < 1:521355/68‑1 (MQ=255) accaccCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGt < 1:2538564/68‑1 (MQ=255) accaccCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGGTAAATGGTTTTTTTCggg > 1:2829622/1‑67 (MQ=255) ccGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGGGGGGGGTTAAAGGGTTTTTTTCGGTcccc > 1:430418/1‑67 (MQ=255) ccGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGGTAAAAGGATTTTTTCCGTTgcgc > 1:3482963/1‑68 (MQ=255) cAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGc < 1:3112681/68‑1 (MQ=255) cAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGc < 1:756054/68‑1 (MQ=255) agagCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGGTAAATGGGTTTTTTTGGTTTCGCACAGCg > 1:1969392/1‑68 (MQ=255) agagCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGGATAATGGGTGTTCTTCGTTCCGCGAACCg > 1:2502535/1‑68 (MQ=255) agCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGTTAAATGGGTTTTCTCGGTTCCCCACAGCgcg > 1:268182/1‑66 (MQ=255) agCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGGGAAATTGGTTTTTTTGGTTCCGCAAAACGag > 1:1674475/1‑67 (MQ=255) agCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGATAAAAGGGTTTTTTCTGGTCCCCCAAGCGaa > 1:2369963/1‑67 (MQ=255) cTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGCTGCGCa < 1:2055478/68‑1 (MQ=255) cTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCa < 1:32978/68‑1 (MQ=255) ggAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATc < 1:1738084/68‑1 (MQ=255) gAAGATGGCTCTTATCGGCT‑GGGGGGGCGGAAATGGTTTTTTTTGGTTCCCCCAAACCATGCGCTTCt > 1:2870496/1‑68 (MQ=255) gATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGt < 1:2587750/68‑1 (MQ=255) gATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGt < 1:604101/68‑1 (MQ=255) ctctTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTgg < 1:1157696/68‑1 (MQ=255) tCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGa < 1:3135938/68‑1 (MQ=255) tCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGa < 1:610059/68‑1 (MQ=255) t‑ggtggGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCGTCGGGTGCTGGCGCAGACcgcg < 1:2729350/68‑1 (MQ=255) tggGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGggt < 1:1974960/68‑1 (MQ=255) | GTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGT > NC_000913/4414843‑4414973 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |