| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 310,934 | 0 | A | C | 29.3% | 80.3 / 10.7 | 42 | F135V (TTC→GTC) | ecpR | DNA‑binding transcriptional dual regulator MatA |
| Reads supporting (aligned to +/- strand): ref base A (20/9); new base C (0/12); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.32e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCC > NC_000913/310867‑311001 | gCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGaa > 1:3247112/1‑68 (MQ=255)gCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGaa > 1:251150/1‑68 (MQ=255)gCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGaa > 1:3232857/1‑68 (MQ=255)gCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGaa > 1:747513/1‑68 (MQ=255) ccGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTNAGGAAc < 1:2036981/68‑1 (MQ=255) ccGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAAc > 1:395726/1‑68 (MQ=255) ccGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAAc > 1:635555/1‑68 (MQ=255) ggATAATTTGCATTTCCCGGTCCGTTTTTTTATTTTTCTTAATATCTTTTCTCAGGACCCTGCCATTa < 1:115134/68‑1 (MQ=255) ggATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTa > 1:2822276/1‑68 (MQ=255) ggATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTa > 1:2921662/1‑68 (MQ=255) aTAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAAt < 1:2968038/68‑1 (MQ=255) ttttccatttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCACAGAACCCTGCCATTAATcac < 1:2967182/67‑1 (MQ=255) atttccatttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAGCCTGCCATTAATcac < 1:213090/68‑1 (MQ=255) atttccatttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATcac < 1:1394867/68‑1 (MQ=255) atttccattcccCGGTCCGTGTTTTTATCTTTCTTAATATCTTTTCTGAGGAACCTGCCATTAATcac < 1:3308702/68‑1 (MQ=255) atttccattcccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATcac < 1:73988/68‑1 (MQ=255) tccatttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATa > 1:2442634/1‑68 (MQ=255) catttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGa < 1:1100179/68‑1 (MQ=255) catttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGa < 1:2628506/68‑1 (MQ=255) catttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGa < 1:3277982/68‑1 (MQ=255) catttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGa < 1:2600805/68‑1 (MQ=255) catttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGa < 1:1303434/68‑1 (MQ=255) catttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCCCAGGAACCTGCCATTAATCACATAGa < 1:3255848/68‑1 (MQ=255) cattcccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGa < 1:1131319/68‑1 (MQ=255) cattcccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGa < 1:2732803/68‑1 (MQ=255) atttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGAc > 1:1568541/1‑68 (MQ=255) tttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGAcc > 1:544945/1‑68 (MQ=255) cccGGTCCGTGGTTTTTTTTTTTTTAATTTTTTTTCCCAGGAACCTGCCATTAATCACATAGACCAGt < 1:414444/68‑1 (MQ=255) cccGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGt < 1:839919/68‑1 (MQ=255) cccGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGt < 1:1540296/68‑1 (MQ=255) ccGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGtt > 1:3240178/1‑68 (MQ=255) aTTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTCTGTAat > 1:637419/1‑68 (MQ=255) ttttATCTTTCTTAATAGCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAata < 1:1966711/68‑1 (MQ=255) tttATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAatat > 1:2726359/1‑68 (MQ=255) ttATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATc < 1:3451893/68‑1 (MQ=255) aTCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCAc > 1:372760/1‑68 (MQ=255) aTCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCAc > 1:2511698/1‑68 (MQ=255) tctttcttAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACg < 1:562958/68‑1 (MQ=255) tctttcttAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACg > 1:415289/1‑68 (MQ=255) ttttttAATATCTTTCCCAAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGAc < 1:3151944/68‑1 (MQ=255) tttAATTTCTTTCCCAAGAACCCTGCTATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCa < 1:2722629/67‑1 (MQ=255) cttAATATTTTTTCTCAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCa < 1:718122/68‑1 (MQ=255) tAATTTCTTTTCTCAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:2795896/68‑1 (MQ=255) tAATATTTTTTCTAAGAAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:1621973/68‑1 (MQ=255) tAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:2272449/68‑1 (MQ=255) tAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:1903088/68‑1 (MQ=255) tAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:443749/68‑1 (MQ=255) tAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:2195209/68‑1 (MQ=255) tAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:2965193/68‑1 (MQ=255) tAATATCTTTTCTCAGCAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:321438/68‑1 (MQ=255) aaTATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTc > 1:1251122/1‑68 (MQ=255) tttttttCACAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAg < 1:1833877/65‑1 (MQ=255) ttCTTTTGTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAg < 1:247638/67‑1 (MQ=255) atCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAg > 1:171583/1‑68 (MQ=255) atCTTTTCTCAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAg < 1:1932396/68‑1 (MQ=255) ttttttCTCGGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:1433800/66‑1 (MQ=255) ttttttCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTTAGTCCAGc < 1:919826/66‑1 (MQ=255) ttttttCTCAGGAACCTGCCATTAATCACATATACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:2668298/66‑1 (MQ=255) ttttttCCCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:2067950/66‑1 (MQ=255) tCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:1564821/68‑1 (MQ=255) tCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc > 1:2805995/1‑68 (MQ=255) tCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc > 1:2642181/1‑68 (MQ=255) tCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc > 1:1787494/1‑68 (MQ=255) tCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc > 1:1357106/1‑68 (MQ=255) tCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc > 1:1215143/1‑68 (MQ=255) tCTTTTCTCAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:3008478/68‑1 (MQ=255) tCTTTTCCCAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:1539402/68‑1 (MQ=255) tCTTTCCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:1979935/68‑1 (MQ=255) tCTTTCCTAAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:1120002/68‑1 (MQ=255) ttttCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGt < 1:3248939/68‑1 (MQ=255) ttttCCCAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGt < 1:419009/68‑1 (MQ=255) ggAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACg > 1:2632218/1‑68 (MQ=255) aCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGccc > 1:225860/1‑68 (MQ=255) | GCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCC > NC_000913/310867‑311001 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |