Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	123.1 / NA	34	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/12);  total (22/12)

CACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTC  >  NC_000913/1905696‑1905825                                                                  |                                                                 cACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATg                                                                <  1:2825506/68‑1 (MQ=255)  aCTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                               >  1:3182984/1‑68 (MQ=255)     gccaCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                            <  1:769796/65‑1 (MQ=255)     gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                            >  1:669911/1‑68 (MQ=255)     gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                            <  1:3005258/68‑1 (MQ=255)     gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                            >  1:2002480/1‑68 (MQ=255)        aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCt                                                         >  1:1088399/1‑68 (MQ=255)        aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCt                                                         >  1:73892/1‑68 (MQ=255)        aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCt                                                         >  1:2549595/1‑68 (MQ=255)         cTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCGTCAATCGGTAAAGATGCCACCCTc                                                        <  1:1444210/68‑1 (MQ=255)          tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                       >  1:883312/1‑68 (MQ=255)          tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                       <  1:3270581/68‑1 (MQ=255)          tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                       <  1:156456/68‑1 (MQ=255)          tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                       >  1:2298243/1‑68 (MQ=255)               ttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATaaa                                                  >  1:2490858/1‑68 (MQ=255)                        ttGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAttt                                         <  1:2074860/68‑1 (MQ=255)                            tATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTg                                     >  1:2410205/1‑68 (MQ=255)                            tATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTg                                     >  1:769725/1‑68 (MQ=255)                                tCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                                 <  1:3128361/68‑1 (MQ=255)                                 cGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGct                                >  1:2613823/1‑67 (MQ=255)                                  gatggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAt                               >  1:225850/1‑68 (MQ=255)                                      gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg                           >  1:1509341/1‑68 (MQ=255)                                      gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg                           >  1:640144/1‑68 (MQ=255)                                        tgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGaa                         >  1:3328491/1‑68 (MQ=255)                                        tgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGaa                         >  1:1030006/1‑68 (MQ=255)                                        tgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGaa                         <  1:743832/68‑1 (MQ=255)                                             ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                    >  1:3124392/1‑68 (MQ=255)                                             ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                    <  1:1896277/68‑1 (MQ=255)                                             ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                    <  1:245895/68‑1 (MQ=255)                                               gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACGGGCCt                  >  1:1209095/1‑68 (MQ=255)                                               gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCt                  >  1:2891442/1‑68 (MQ=255)                                                 tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTa                >  1:2726451/1‑68 (MQ=255)                                                  gcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt               <  1:1635739/68‑1 (MQ=255)                                                         tCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGt        >  1:1863854/1‑68 (MQ=255)                                                              aaaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGACCCGGCCTTATTTTTGGTGCCGt   <  1:634632/68‑1 (MQ=255)                                                               aaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTc  >  1:2909343/1‑68 (MQ=255)                                                                  |                                                                 CACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTC  >  NC_000913/1905696‑1905825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑