Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	128.5 / NA	35	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/11);  total (24/11)

AACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCT  >  NC_000913/4390692‑4390814                                                               |                                                             aaCTCACGCCCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                         <  1:1219493/68‑1 (MQ=255) aaCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                         <  1:1946740/68‑1 (MQ=255) aaCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                         <  1:2022569/68‑1 (MQ=255)    tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                      >  1:1722188/1‑68 (MQ=255)         cACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                 <  1:1236137/68‑1 (MQ=255)          aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGtgtg                                                >  1:829556/1‑68 (MQ=255)             ccTGGGGAGTCAATCCCATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTTTGCTg                                             <  1:2005425/68‑1 (MQ=255)                  ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTCTGCTGACCgc                                        >  1:2742528/1‑67 (MQ=255)                   gAGTAAACCTCATCCCCGCCGTGGGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                       <  1:1300016/68‑1 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                      >  1:2820398/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                      >  1:1916666/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                      >  1:3437330/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                      >  1:690539/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                      >  1:703876/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                      >  1:2947094/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                      >  1:1205831/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                      >  1:900840/1‑68 (MQ=255)                          ccacaccccCGCCGGGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAg                                <  1:2247017/67‑1 (MQ=255)                            acatcacCACCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAgct                              >  1:3280727/1‑66 (MQ=255)                              atcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGt                            >  1:1526557/1‑68 (MQ=255)                               tcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTc                           >  1:58067/1‑68 (MQ=255)                               tcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGCCGCGGGGTGCTGACCGAGCCCCGAGTTGTc                           >  1:1397010/1‑68 (MQ=255)                                  ccgccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCaga                        <  1:866608/68‑1 (MQ=255)                                  ccgacgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCaga                        <  1:3283195/68‑1 (MQ=255)                                   cgccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCagag                       <  1:1785493/68‑1 (MQ=255)                                   cgccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCCTGGTGTGCTGACTGAGTCCCGAGTTGTCagag                       <  1:3193646/68‑1 (MQ=255)                                    gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAgaga                      >  1:465677/1‑68 (MQ=255)                                    gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAgaga                      >  1:972348/1‑68 (MQ=255)                                    gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAgaga                      >  1:1729712/1‑68 (MQ=255)                                           gggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGtt               >  1:1330915/1‑68 (MQ=255)                                            ggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg              >  1:3109737/1‑68 (MQ=255)                                             gaggTGATGCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTgg             <  1:1030697/65‑1 (MQ=255)                                              aaGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGt            <  1:136002/68‑1 (MQ=255)                                              aaGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGt            <  1:1226795/68‑1 (MQ=255)                                               aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTc           >  1:582101/1‑68 (MQ=255)                                               aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTc           >  1:141848/1‑68 (MQ=255)                                                  gATACAGCCGAGCGTCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg        <  1:62240/68‑1 (MQ=255)                                                     aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc     >  1:2603959/1‑68 (MQ=255)                                                       aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc   >  1:1050714/1‑68 (MQ=255)                                                        gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATctct  >  1:1489335/1‑68 (MQ=255)                                                               |                                                             AACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCT  >  NC_000913/4390692‑4390814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑