Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	108.1 / NA	30	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/8);  total (22/8)

AACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTT  >  NC_000913/4390692‑4390816                                                               |                                                               aaCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                           <  1:1508636/68‑1 (MQ=255)  aCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTc                                                          >  1:1870012/1‑68 (MQ=255)    tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                        <  1:3018728/68‑1 (MQ=255)         cACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                   <  1:834852/68‑1 (MQ=255)          aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGtgtg                                                  <  1:2950111/68‑1 (MQ=255)          aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGtgtg                                                  >  1:2490559/1‑68 (MQ=255)          aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGCGGtgtg                                                  >  1:1429502/1‑68 (MQ=255)              cgggggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGa                                              <  1:3193916/66‑1 (MQ=255)               tGGGGAGTCAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAc                                             <  1:477140/68‑1 (MQ=255)                  ggAGTCNATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGa                                          >  1:2103922/1‑68 (MQ=255)                  ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGa                                          >  1:1928325/1‑68 (MQ=255)                  ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGa                                          >  1:2682435/1‑68 (MQ=255)                  ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGa                                          >  1:1367632/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGTGGTCGTGGTGTGCTGACCGAGc                                        >  1:2403302/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                        >  1:813831/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                        >  1:2653631/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                        >  1:910149/1‑68 (MQ=255)                    aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGATGTGCTGACCGAGc                                        >  1:306590/1‑68 (MQ=255)                    aGTCAANCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                        >  1:2105056/1‑68 (MQ=255)                       cAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGCCGTGGTGTGCTGACCGAGcccc                                     >  1:1238833/1‑68 (MQ=255)                          tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAg                                  >  1:3207290/1‑68 (MQ=255)                                   cgccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCagag                         <  1:2988223/68‑1 (MQ=255)                                      cgTGGGGGAGGTGATCCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGata                      <  1:3086481/68‑1 (MQ=255)                                      cgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGata                      >  1:2291037/1‑68 (MQ=255)                                        tGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                    <  1:1339288/68‑1 (MQ=255)                                           gggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGtt                 >  1:2400535/1‑68 (MQ=255)                                           gggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGtt                 >  1:3452148/1‑68 (MQ=255)                                                gTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCa            <  1:3134236/68‑1 (MQ=255)                                                     aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc       >  1:255893/1‑68 (MQ=255)                                                       aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc     >  1:1564160/1‑68 (MQ=255)                                                       aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc     <  1:1155164/68‑1 (MQ=255)                                                        gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctcc    >  1:2838377/1‑67 (MQ=255)                                                        gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATctct    >  1:1411973/1‑68 (MQ=255)                                                        gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATctct    >  1:1319349/1‑68 (MQ=255)                                                          cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCttt  >  1:431203/1‑68 (MQ=255)                                                               |                                                               AACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTCTTT  >  NC_000913/4390692‑4390816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑